HPRT Antibodies
We offer HPRT Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each HPRT Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HPRT Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the HPRT Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HPRT polyclonal antibodies and browse our HPRT monoclonal antibody catalog.
Alternate Names for HPRT Antibodies
anti-HPRT antibody, anti-HPRT1 antibody, anti-EC 2.4.2.8 antibody, anti-HGPRTHPRTHGPRTase antibody, anti-hypoxanthine phosphoribosyltransferase 1 antibody, anti-hypoxanthine-guanine phosphoribosyltransferase antibody
13 Results for "hprt" in Primary Antibodies
![Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0015.jpg "Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33527-img0002.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Rat colon. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0013.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGEHPRT antibody dilution: 1:500](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0009.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HPRT Antibody [NBP1-33527] - Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-33527-img0014.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Human endometrial cancer. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0005.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Colon cancer. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0006.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Rat brain. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0007.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Mouse colon. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0012.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.HPRT stained by HPRT antibody (NBP1-33527) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33527_rabbit-polyclonal-hprt-antibody-910202420115025.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - HPRT antibody detects HPRT protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.HPRT stained by HPRT antibody (NBP1-33527) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33527_rabbit-polyclonal-hprt-antibody-910202420104479.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: HPRT Antibody [NBP1-33527] - Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (10 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33527_rabbit-polyclonal-hprt-antibody-1610202419414546.jpg)
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0007.jpg "Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Western blot analysis of HPRT on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: 293 cell lysate Lane 2: Hela cell lysate Lane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0004.jpg)
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Hela cell using anti-HPRT antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: A431Lane 3: MCF-7 Lane 4: 293Lane 5: Rat kidney Lane 6: Rat brainLane 7: Mouse testis Lane 8: Mouse colon](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0005.jpg)
![Western Blot: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Western-Blot-NBP2-75528-img0006.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0001.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0002.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody (JU03-26) [NBP2-75528] - Analysis of paraffin-embedded human kidney tissue using anti-HPRT antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HPRT-Antibody-JU03-26-Immunohistochemistry-Paraffin-NBP2-75528-img0003.jpg)
![Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Western-Blot-NBP2-37245-img0004.jpg "Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.")
![Immunohistochemistry-Paraffin: HPRT Antibody (5F11A7) [NBP2-37245] - Analysis of kidney tissues using HPRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Immunohistochemistry-NBP2-37245-img0006.jpg)
![Flow (Intracellular): HPRT Antibody (5F11A7) [NBP2-37245] - An intracellular stain was performed on HeLa cells with HPRT Antibody (5F11A7) NBP2-37245APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Flow-Intracellular-NBP2-37245-img0007.jpg)
![Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against human HPRT1 (AA: FULL(1-218)) recombinant protein. (Expected MW is 50.5 kDa)](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Western-Blot-NBP2-37245-img0002.jpg)
![Western Blot: HPRT Antibody (5F11A7) [NBP2-37245] - Western blot analysis using HPRT1 mAb against HEK293 (1) and HPRT1 (AA: FULL(1-218))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Western-Blot-NBP2-37245-img0003.jpg)
![Flow Cytometry: HPRT Antibody (5F11A7) [NBP2-37245] - Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-Flow-Cytometry-NBP2-37245-img0005.jpg)
![ELISA: HPRT Antibody (5F11A7) [NBP2-37245] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/HPRT-Antibody-5F11A7-ELISA-NBP2-37245-img0001.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Analysis in human testis and skeletal muscle tissues. Corresponding HPRT RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Analysis in human testis and skeletal muscle tissues. Corresponding HPRT RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HPRT Antibody [NBP1-92006] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-92006-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-92006] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92006-img0008.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human testis shows very strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0019.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0016.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human kidney shows strong cytoplasmic positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0017.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-92006] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-92006-img0018.jpg)
![Western Blot: HPRT Antibody [NBP3-03361] - Analysis of extracts of various cell lines, using HPRT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP3-03361-img0003.jpg "Western Blot: HPRT Antibody [NBP3-03361] - Analysis of extracts of various cell lines, using HPRT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![<b>Genetic Strategies Validation. </b>Knockout Validated: HPRT Antibody [NBP3-03361] - Analysis of extracts from normal (control) and HPRT1 knockout (KO) 293T cells, using HPRT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/HPRT-Antibody-Knockout-Validated-NBP3-03361-img0002.jpg)
![Western Blot: HPRT Antibody [NBP1-52903] - Hela cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-52903-img0002.jpg "Western Blot: HPRT Antibody [NBP1-52903] - Hela cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of extracts of various cell lines, using HPRT1 Rabbit mAb (NBP3-16702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HPRT-Antibody-4N2Q6-Western-Blot-NBP3-16702-img0001.jpg "Western Blot: HPRT Antibody (4N2Q6) [NBP3-16702] - Western blot analysis of extracts of various cell lines, using HPRT1 Rabbit mAb (NBP3-16702) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01] - HPRT1 monoclonal antibody (M01), clone 4C3-G8 Analysis of HPRT1 expression in MCF-7.](http://images.novusbio.com/fullsize/HPRT-Antibody-4C3-G8-Western-Blot-H00003251-M01-img0002.jpg "Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01] - HPRT1 monoclonal antibody (M01), clone 4C3-G8 Analysis of HPRT1 expression in MCF-7.")
![Western Blot: HPRT Antibody [NBP1-98359] - Mouse Thymus Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/HPRT-Antibody-Western-Blot-NBP1-98359-img0002.jpg "Western Blot: HPRT Antibody [NBP1-98359] - Mouse Thymus Lysate 1.0ug/ml, Gel Concentration: 12%")
![Western Blot: HPRT Antibody (2G8) [NBP1-74045] - The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/HPRT-Antibody-2G8-Western-Blot-NBP1-74045-img0001.jpg "Western Blot: HPRT Antibody (2G8) [NBP1-74045] - The extracts of 293T(35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HPRT (1:500~1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")