HISPPD2A Antibodies
We offer HISPPD2A Antibodies for use in common research applications: Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry. Each HISPPD2A Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our HISPPD2A Antibodies can be used in a variety of model species: Human. Use the list below to choose the HISPPD2A Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our HISPPD2A polyclonal antibodies.
Alternate Names for HISPPD2A Antibodies
anti-HISPPD2A antibody, anti-PPIP5K1 antibody, anti-diphosphoinositol pentakisphosphate kinase 1 antibody, anti-histidine acid phosphatase domain-containing protein 2A antibody, anti-inositol pyrophosphate synthase 1 antibody, anti-VIP1 antibody
6 Results for "hisppd2a" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - Validation of anti-PPIP5K2 and anti-PPIP5K1 antibodies specific in vitro. (A) Anti-PPIP5K2 antibody immunofluorescence signal coincides with the signal produced by GFP-tagged WT PPIP5K2, and p.Arg837His variant harboring PPIP5K2 expressed in COS7 cells. (B) The anti-PPIP5K1 antibody immunofluorescence signal coincides with the GFP-tagged PPIP5K1 signal, but not with fluorescently tagged PPIP5K2 expressed in COS7 cells. (C) Western blot tested indicated the specific bands of sizes that correspond to full-length PPIP5K2 around 140 kDa. (D) Western blot on whole protein lysates from mice tissues with specific variants. Citation: Yousaf R, Gu C, Ahmed ZM, Khan SN, Friedman TB, Riazuddin S, et al. (2018) Mutations in Diphosphoinositol-Pentakisphosphate Kinase PPIP5K2 are associated with hearing loss in human and mouse. PLoS Genet 14(3): e1007297](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89693-img0006.jpg "Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - Validation of anti-PPIP5K2 and anti-PPIP5K1 antibodies specific in vitro. (A) Anti-PPIP5K2 antibody immunofluorescence signal coincides with the signal produced by GFP-tagged WT PPIP5K2, and p.Arg837His variant harboring PPIP5K2 expressed in COS7 cells. (B) The anti-PPIP5K1 antibody immunofluorescence signal coincides with the GFP-tagged PPIP5K1 signal, but not with fluorescently tagged PPIP5K2 expressed in COS7 cells. (C) Western blot tested indicated the specific bands of sizes that correspond to full-length PPIP5K2 around 140 kDa. (D) Western blot on whole protein lysates from mice tissues with specific variants. Citation: Yousaf R, Gu C, Ahmed ZM, Khan SN, Friedman TB, Riazuddin S, et al. (2018) Mutations in Diphosphoinositol-Pentakisphosphate Kinase PPIP5K2 are associated with hearing loss in human and mouse. PLoS Genet 14(3): e1007297")
![Immunohistochemistry-Paraffin: HISPPD2A Antibody [NBP1-89693] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Immunohistochemistry-Paraffin-NBP1-89693-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89693-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HISPPD2A Antibody [NBP1-89693] - HISPPD2A/PPIP5K1 antibody was used at 1:200 dilution for localizing PPIP5K2 protein in sensory and non-sensory cells of mouse inner ear using confocal microscopy analysis. Figure A shows the real-time PCR expression of Ppip5k2 in adult (P150) mice, normalized against Gapdh (Delta CT) and Ppip5k1 expression (Delta CT). In Figure B, a cross-section through one of the coils of inner ear shows diffuse cytoplasmic immunolabeling of PPIP5K2 throughout the cochlear duct, including spiral ganglion neurons (SG), organ of Corti (OC), and stria vascularis (SV). Figure C shows the expression of PPIP5K2 at the ages tested in WT mice, from early postnatal day P16, up to 9 months of age. Scale bars: 100um (panel A), and 20um (panel C).](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89693-img0005.jpg)
![Western Blot: HISPPD2A Antibody [NBP2-44293] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.) NBP2-44293 was used for IP at 6 ug per reaction. PPIP5K1 was also immunoprecipitated by NBP2-44292.](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Western-Blot-NBP2-44293-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: HISPPD2A Antibody [NBP2-44293] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer.) NBP2-44293 was used for IP at 6 ug per reaction. PPIP5K1 was also immunoprecipitated by NBP2-44292.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: HISPPD2A Antibody [NBP2-44293] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer.](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Western-Blot-NBP2-44293-img0001.jpg)
![Immunoprecipitation: HISPPD2A Antibody [NBP2-44292] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. HISPPD2A was also immunoprecipitated by NBP2-44293.](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Immunoprecipitation-NBP2-44292-img0001.jpg "<b>Independent Antibodies Validation. </b>Immunoprecipitation: HISPPD2A Antibody [NBP2-44292] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. HISPPD2A was also immunoprecipitated by NBP2-44293.")
![Western Blot: HISPPD2A Antibody [NBP3-09416] - Western blot analysis of HISPPD2A in 786-0 Whole Cell lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Western-Blot-NBP3-09416-img0001.jpg "Western Blot: HISPPD2A Antibody [NBP3-09416] - Western blot analysis of HISPPD2A in 786-0 Whole Cell lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: HISPPD2A Antibody [NBP3-09937] - Western blot analysis of HISPPD2A in 721_B Whole Cell lysates. Antibody dilution at 1.0ug/ml](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Western-Blot-NBP3-09937-img0001.jpg "Western Blot: HISPPD2A Antibody [NBP3-09937] - Western blot analysis of HISPPD2A in 721_B Whole Cell lysates. Antibody dilution at 1.0ug/ml")
![Western Blot: HISPPD2A Antibody [H00009677-B01P] - Analysis of HISPPD2A expression in transfected 293T cell line by HISPPD2A polyclonal antibody. Lane1:HISPPD2A transfected lysate(157.63 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/HISPPD2A-Antibody-Western-Blot-H00009677-B01P-img0002.jpg "Western Blot: HISPPD2A Antibody [H00009677-B01P] - Analysis of HISPPD2A expression in transfected 293T cell line by HISPPD2A polyclonal antibody. Lane1:HISPPD2A transfected lysate(157.63 KDa). Lane2:Non-transfected lysate.")