FXR1 Antibodies
We offer FXR1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Sandwich ELISA, Western Blot. Each FXR1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our FXR1 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the FXR1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our FXR1 polyclonal antibodies and browse our FXR1 monoclonal antibody catalog.
Alternate Names for FXR1 Antibodies
anti-FXR1 antibody, anti-fragile X mental retardation syndrome-related protein 1 antibody, anti-fragile X mental retardation, autosomal homolog 1 antibody, anti-FXR1P antibody, anti-hFXR1p antibody
21 Results for "fxr1" in Primary Antibodies
![Western Blot: FXR1 Antibody [NBP1-89546] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP1-89546-img0010.jpg "Western Blot: FXR1 Antibody [NBP1-89546] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89546-img0011.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0015.jpg)
![Western Blot: FXR1 Antibody [NBP1-89546] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP1-89546-img0009.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0012.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0013.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0014.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Simple Western lane view shows a specific band for FXR1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0006.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Electropherogram image(s) of corresponding Simple Western lane view. FXR1 antibody was used at 1:100 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0007.jpg)
![Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0005.jpg "Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from mouse NIH3T3 (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-FXR1 antibody used for WB at 0.4 ug/ml. Detection: chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: FXR1 Antibody [NBP2-22246] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22246 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-22246-img0001.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22246 used for IP at 6 ug/mg lysate. FXR1 was also immunoprecipitated by rabbit anti-FXR1 antibody. For blotting immunoprecipitated FXR1, NBP2-22246 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0003.jpg)
![Immunoprecipitation: FXR1 Antibody [NBP2-22246] - Adult mouse heart. A: 30 ug whole cell lysate. B: IgG-IP. C: Fxr1-antibody-IP. 10% SDS-PAGE, antibody diluted 1:2000 in WB assay and 3ug/mg in IP assay. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunoprecipitation-NBP2-22246-img0004.jpg)
![Western Blot: FXR1 Antibody [NBP1-33719] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP1-33719-img0005.jpg "Western Blot: FXR1 Antibody [NBP1-33719] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: FXR1 Antibody [NBP1-33719] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP1-33719-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunocytochemistry-immunofluorescence-8112023132213..jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Analysis of paraffin-embedded sections of zebrafish kidney tube, using FXR1 antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132323..jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Mouse kidney.FXR1 stained by FXR1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132436..jpg)
![Western Blot: FXR1 Antibody [NBP1-33719] - Sample (30 ug of whole cell lysate) A: zebrafish brain 7.5% SDS PAGE NBP1-33719 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-33719_rabbit-polyclonal-fxr1-antibody-910202420134079.jpg)
![Western Blot: FXR1 Antibody (PSH01-67) [NBP3-32356] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse skeletal muscle tissue lysate Lane 11: Rat skeletal muscle tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-278202415212450.jpg "Western Blot: FXR1 Antibody (PSH01-67) [NBP3-32356] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lane 10: Mouse skeletal muscle tissue lysate Lane 11: Rat skeletal muscle tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/10,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-67) [NBP3-32356] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32356) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-298202414333619.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-67) [NBP3-32356] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32356) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-29202410484842.jpg)
![Flow Cytometry: FXR1 Antibody (PSH01-67) [NBP3-32356] - Flow cytometric analysis of NIH/3T3 cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32356, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32356_rabbit-fxr1-mab-psh01-67-392024884678.jpg)
![Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-278202416331261.jpg "Western Blot: FXR1 Antibody (JE40-57) [NBP3-32354] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: A549 cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: HeLa cell lysate Lane 7: C2C12 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 1 minute 55 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32354) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29820241492613.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (JE40-57) [NBP3-32354] - Immunocytochemistry analysis of HeLa cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32354) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-29202410334066.jpg)
![Flow Cytometry: FXR1 Antibody (JE40-57) [NBP3-32354] - Flow cytometric analysis of HeLa cells labeling FXR1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32354, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32354_rabbit-fxr1-mab-je40-57-392024884674.jpg)
![Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-278202415295525.jpg "Western Blot: FXR1 Antibody (PSH01-35) [NBP3-32355] - Western blot analysis of FXR1 on different lysates with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: HepG2 cell lysate Lane 4: A549 cell lysate Lane 5: K-562 cell lysate Lane 6: COS-1 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C2C12 cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 70 kDa Observed band size: 70/75 kDa Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32355) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32355) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-29820241435234.jpg)
![Immunocytochemistry/ Immunofluorescence: FXR1 Antibody (PSH01-35) [NBP3-32355] - Immunocytochemistry analysis of PC-12 cells labeling FXR1 with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FXR1 antibody (NBP3-32355) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32355_rabbit-fxr1-mab-psh01-35-2920241192383.jpg)
![Western Blot: FXR1 Antibody (2S2B1) [NBP3-16681] - Western blot analysis of extracts of various cell lines, using FXR1 Rabbit mAb (NBP3-16681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/FXR1-Antibody-2S2B1-Western-Blot-NBP3-16681-img0004.jpg "Western Blot: FXR1 Antibody (2S2B1) [NBP3-16681] - Western blot analysis of extracts of various cell lines, using FXR1 Rabbit mAb (NBP3-16681) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody (2S2B1) [NBP3-16681] - Immunofluorescence analysis of U-2 OS cells using FXR1 Rabbit mAb (NBP3-16681) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FXR1-Antibody-2S2B1-Immunocytochemistry-Immunofluorescence-NBP3-16681-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody (2S2B1) [NBP3-16681] - Immunofluorescence analysis of C6 cells using FXR1 Rabbit mAb (NBP3-16681) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FXR1-Antibody-2S2B1-Immunocytochemistry-Immunofluorescence-NBP3-16681-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody (2S2B1) [NBP3-16681] - Immunofluorescence analysis of NIH-3T3 cells using FXR1 Rabbit mAb (NBP3-16681) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FXR1-Antibody-2S2B1-Immunocytochemistry-Immunofluorescence-NBP3-16681-img0002.jpg)
![Western Blot: FXR1 Antibody [NB100-41388] - 1ug/ml staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NB100-41388-img0004.jpg "Western Blot: FXR1 Antibody [NB100-41388] - 1ug/ml staining of NIH-3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NB100-41388] - Paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41388-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NB100-41388] - Paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41388-img0005.jpg)
![Western Blot: FXR1 Antibody (2G11) [H00008087-M01] - Analysis of FXR1 expression in transfected 293T cell line by FXR1 monoclonal antibody (M01), clone 2G11.Lane 1: FXR1 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FXR1-Antibody-2G11-Western-Blot-H00008087-M01-img0007.jpg "Western Blot: FXR1 Antibody (2G11) [H00008087-M01] - Analysis of FXR1 expression in transfected 293T cell line by FXR1 monoclonal antibody (M01), clone 2G11.Lane 1: FXR1 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: FXR1 Antibody (2G11) [H00008087-M01] - Analysis of monoclonal antibody to FXR1 on formalin-fixed paraffin-embedded human colon adenocarcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/FXR1-Antibody-2G11-Immunohistochemistry-Paraffin-H00008087-M01-img0005.jpg)
![Sandwich ELISA: FXR1 Antibody (2G11) [H00008087-M01] - Detection limit for recombinant GST tagged FXR1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FXR1-Antibody-2G11-Sandwich-ELISA-H00008087-M01-img0006.jpg)
![Western Blot: FXR1 Antibody [NBP2-99343] - Anti-FXR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 72 kDa](http://images.novusbio.com/fullsize/FXR1-Antibody-Western-Blot-NBP2-99343-img0004.jpg "Western Blot: FXR1 Antibody [NBP2-99343] - Anti-FXR1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 72 kDa")
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP2-99343] - Immunofluorescence staining of FXR1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FXR1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99343-img0001.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP2-99343] - Immunochemical staining of human FXR1 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP2-99343-img0003.jpg)
![Western Blot: FXR1 Antibody (SR2140) [NBP3-22299] - Western blot analysis using NBP3-22299 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22299_rabbit-fxr1-mab-sr2140-57202315521840.jpg "Western Blot: FXR1 Antibody (SR2140) [NBP3-22299] - Western blot analysis using NBP3-22299 in MCF7 cell lysate.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP2-98798] - Immunochemical staining FXR1 in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP2-98798-img0002.jpg "Immunohistochemistry-Paraffin: FXR1 Antibody [NBP2-98798] - Immunochemical staining FXR1 in mouse skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP2-98798] - Immunochemical staining FXR1 in human skin with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP2-98798-img0001.jpg)