eIF4G1 Antibodies
We offer eIF4G1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each eIF4G1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our eIF4G1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the eIF4G1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our eIF4G1 polyclonal antibodies and browse our eIF4G1 monoclonal antibody catalog.
Alternate Names for eIF4G1 Antibodies
anti-eIF4G1 antibody, anti-DKFZp686A1451 antibody, anti-eIF-4-gamma 1 antibody, anti-eIF-4G 1 antibody, anti-EIF-4G1 antibody, anti-EIF4-gamma antibody, anti-EIF4F antibody, anti-EIF4GI antibody, anti-EIF4Gp220 antibody, anti-eucaryotic translation initiation factor 4G antibody, anti-eukaryotic translation initiation factor 4 gamma 1 antibody, anti-eukaryotic translation initiation factor 4 gamma, 1 antibody, anti-P220 antibody
12 Results for "eif4g1" in Primary Antibodies
![Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Western-Blot-NB100-268-img0001.jpg "Western Blot: eIF4G1 Antibody [NB100-268] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: eIF4G1 Antibody [NB100-268] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eiF4G1/eiF4GI antibody used for IP at 6 ug per reaction. eiF4G1/eiF4GI was also immunoprecipitated by rabbit antieiF4G1/eiF4GI antibody. For blotting immunoprecipitated eiF4G1/eiF4GI, rabbit antieiF4G1/eiF4GI antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunoprecipitation-NB100-268-img0003.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NBP1-04965 or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Immunohistochemistry-NBP1-04965-img0004.jpg "Immunohistochemistry-Paraffin: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NBP1-04965 or the same antibody preincubated with blocking peptide(right).")
![Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Western-Blot-NBP1-04965-img0002.jpg)
![Western Blot: eIF4G1 [p Ser1231] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1231]-Antibody-Western-Blot-NBP1-04965-img0003.jpg)
![Western Blot: eIF4G1 Antibody [NBP1-84868] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Western-Blot-NBP1-84868-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: eIF4G1 Antibody [NBP1-84868] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: eIF4G1 Antibody [NBP1-84868] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84868-img0006.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 Antibody [NBP1-84868] - Staining of human testis shows moderate to strong cytoplasmic positivity in spermatids.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunohistochemistry-Paraffin-NBP1-84868-img0011.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 Antibody [NBP1-84868] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunohistochemistry-Paraffin-NBP1-84868-img0009.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 Antibody [NBP1-84868] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunohistochemistry-Paraffin-NBP1-84868-img0010.jpg)
![Immunohistochemistry-Paraffin: eIF4G1 Antibody [NBP1-84868] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunohistochemistry-Paraffin-NBP1-84868-img0012.jpg)
![Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody (M10), clone 2A9. Lane 1: EIF4G1 transfected lysatE (70.95 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-2A9-Western-Blot-H00001981-M10-img0012.jpg "Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody (M10), clone 2A9. Lane 1: EIF4G1 transfected lysatE (70.95 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of monoclonal antibody to EIF4G1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/eIF4G1-Antibody-2A9-Immunocytochemistry-Immunofluorescence-H00001981-M10-img0008.jpg)
![Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/eIF4G1-Antibody-2A9-Western-Blot-H00001981-M10-img0009.jpg)
![Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in HepG2 (Cat # L019V1).](http://images.novusbio.com/fullsize/eIF4G1-Antibody-2A9-Western-Blot-H00001981-M10-img0010.jpg)
![Western Blot: eIF4G1 Antibody (2A9) [H00001981-M10] - Analysis of EIF4G1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/eIF4G1-Antibody-2A9-Western-Blot-H00001981-M10-img0011.jpg)
![ELISA: eIF4G1 Antibody (2A9) [H00001981-M10] - Detection limit for recombinant GST tagged EIF4G1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-2A9-ELISA-H00001981-M10-img0006.jpg)
![Western Blot: eIF4G1 Antibody [NB100-93317] - Detection of Human eIF4G1 on HeLa whole cell lysate using NB100-93317. eIF4G1 was also immunoprecipitated by rabbit anti-eIF4G1 antibodies NB100-93315 and NB100-93316.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Western-Blot-NB100-93317-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: eIF4G1 Antibody [NB100-93317] - Detection of Human eIF4G1 on HeLa whole cell lysate using NB100-93317. eIF4G1 was also immunoprecipitated by rabbit anti-eIF4G1 antibodies NB100-93315 and NB100-93316.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: eIF4G1 Antibody [NB100-93317] - Human ovarian carcinoma. Antibody used at a dilution of 1:200.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunohistochemistry-Paraffin-NB100-93317-img0003.jpg)
![Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01] - EIF4G1 monoclonal antibody (M01), clone 3A10. Analysis of EIF4G1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-3A10-Western-Blot-H00001981-M01-img0005.jpg "Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01] - EIF4G1 monoclonal antibody (M01), clone 3A10. Analysis of EIF4G1 expression in NIH/3T3.")
![Western Blot: eIF4G1 Antibody (3A10) [H00001981-M01] - EIF4G1 monoclonal antibody (M01), clone 3A10. Analysis of EIF4G1 expression in IMR-32.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-3A10-Western-Blot-H00001981-M01-img0004.jpg)
![ELISA: eIF4G1 Antibody (3A10) [H00001981-M01] - Detection limit for recombinant GST tagged EIF4G1 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-3A10-ELISA-H00001981-M01-img0006.jpg)
![Western Blot: eIF4G1 [p Ser1108] Antibody (PS01-08) [NBP3-32764] - Western blot analysis of eIF4G1 on different lysates with Rabbit anti-eIF4G1 antibody (NBP3-32764) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 175 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32764) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32764_rabbit-eif4g1-p-ser1108-mab-ps01-08-288202412442973.jpg "Western Blot: eIF4G1 [p Ser1108] Antibody (PS01-08) [NBP3-32764] - Western blot analysis of eIF4G1 on different lysates with Rabbit anti-eIF4G1 antibody (NBP3-32764) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SK-Br-3 cell lysate Lane 3: MCF-7 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 175 kDa Observed band size: 250 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32764) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: eIF4G1 [p Ser1108] Antibody (PS01-08) [NBP3-32764] - Immunocytochemistry analysis of A431 cells labeling eIF4G1 with Rabbit anti-eIF4G1 antibody (NBP3-32764) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF4G1 antibody (NBP3-32764) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32764_rabbit-eif4g1-p-ser1108-mab-ps01-08-29202410484829.jpg)
![Western Blot: eIF4G1 [p Ser1108] Antibody [NBP3-05453] - Western blot analysis of extracts of NIH/3T3 cells, using eIF4G1 antibody (NBP3-05453) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/eIF4G1-[p-Ser1108]-Antibody-Western-Blot-NBP3-05453-img0001.jpg "Western Blot: eIF4G1 [p Ser1108] Antibody [NBP3-05453] - Western blot analysis of extracts of NIH/3T3 cells, using eIF4G1 antibody (NBP3-05453) at 1:1000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: eIF4G1 Antibody [NBP2-55105] - Staining of human cell line A-431 shows localization to cytosol & vesicles.](http://images.novusbio.com/fullsize/eIF4G1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55105-img0001.jpg "Immunocytochemistry/Immunofluorescence: eIF4G1 Antibody [NBP2-55105] - Staining of human cell line A-431 shows localization to cytosol & vesicles.")