eIF4E Antibodies
We offer eIF4E Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each eIF4E Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our eIF4E Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the eIF4E Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our eIF4E polyclonal antibodies and browse our eIF4E monoclonal antibody catalog.
Alternate Names for eIF4E Antibodies
anti-eIF4E antibody, anti-CBP antibody, anti-eIF-4E antibody, anti-eIF-4F 25 kDa subunit antibody, anti-EIF4E1 antibody, anti-EIF4EL1MGC111573 antibody, anti-EIF4F antibody, anti-eukaryotic translation initiation factor 4E antibody, anti-eukaryotic translation initiation factor 4E-like 1 antibody, anti-mRNA cap-binding protein antibody
20 Results for "eif4e" in Primary Antibodies
![Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Western-Blot-NB100-81898-img0009.jpg "Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using NB100-81898")
![Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using NB100-81898](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81898-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81898 (left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/eIF4E-[p-Ser209]-Antibody-Immunohistochemistry-NB100-81898-img0006.jpg)
![Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NB100-58833-img0002.jpg "Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: eIF4E Antibody [NB100-58833] - Detection of human eIF4E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-eIF4E antibody NB100-58833 used for IP at 3 ug per reaction. eIF4E was also immunoprecipitated by another rabbit anti-eIF4E antibody. For blotting immunoprecipitated eIF4E, NB100-58833 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunoprecipitation-NB100-58833-img0003.jpg)
![Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Western-Blot-NBP2-22177-img0006.jpg "Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (5D11) [NBP2-22177] - Immunofluorescence analysis of GC-7901 cells using eIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunocytochemistry-Immunofluorescence-NBP2-22177-img0002.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using eIF4E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-22177-img0004.jpg)
![Flow Cytometry: eIF4E Antibody (5D11) [NBP2-22177] - Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Flow-Cytometry-NBP2-22177-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using eIF4E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-Immunohistochemistry-Paraffin-NBP2-22177-img0003.jpg)
![ELISA: eIF4E Antibody (5D11) [NBP2-22177] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/eIF4E-Antibody-5D11-ELISA-NBP2-22177-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Immunocytochemistry-Immunofluorescence-NBP3-07141-img0006.jpg "Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).")
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed U87 cells. eIF4E antibody (PCRP-EIF4E-1D3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Flow-Cytometry-NBP3-07141-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Immunocytochemistry-Immunofluorescence-NBP3-07141-img0005.jpg)
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed HeLa cells. eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Flow-Cytometry-NBP3-07141-img0002.jpg)
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Flow cytometric analysis of PFA-fixed Raji cells.eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Flow-Cytometry-NBP3-07141-img0003.jpg)
![Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) [NBP3-07141] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Protein-Array-NBP3-07141-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08884-img0004.jpg "Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).")
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Flow cytometric analysis of PFA-fixed U87 cells. eIF4E antibody (PCRP-EIF4E-1D3) followed by goat anti-mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Azide-and-BSA-Free-Flow-Cytometry-NBP3-08884-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green), phalloidin (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP3-08884-img0005.jpg)
![Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Flow cytometric analysis of PFA-fixed Raji cells.eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (blue), isotype control (red).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Azide-and-BSA-Free-Flow-Cytometry-NBP3-08884-img0002.jpg)
![Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3).](http://images.novusbio.com/fullsize/eIF4E-Antibody-PCRP-EIF4E-1D3-Azide-and-BSA-Free-Protein-Array-NBP3-08884-img0006.jpg)
![Western Blot: eIF4E Antibody (JE60-70) [NBP3-32302] - Western blot analysis of eIF4E on different lysates with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32302) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-278202415212480.jpg "Western Blot: eIF4E Antibody (JE60-70) [NBP3-32302] - Western blot analysis of eIF4E on different lysates with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32302) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF4E Antibody (JE60-70) [NBP3-32302] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32302) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-298202414161811.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (JE60-70) [NBP3-32302] - Immunocytochemistry analysis of SK-Br-3 cells labeling eIF4E with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF4E antibody (NBP3-32302) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-2920241192325.jpg)
![Flow Cytometry: eIF4E Antibody (JE60-70) [NBP3-32302] - Flow cytometric analysis of SK-Br-3 cells labeling eIF4E. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32302, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32302_rabbit-eif4e-mab-je60-70-29202416453285.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195] - C-terminal region validated by WB using Mouse Brain lysate.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0005.jpg "Western Blot: eIF4E Antibody [NBP1-57195] - C-terminal region validated by WB using Mouse Brain lysate.")
![Immunohistochemistry-Paraffin: eIF4E Antibody [NBP1-57195] - Human Lung respiratory epithelium tissue, 5ug/ml.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunohistochemistry-Paraffin-NBP1-57195-img0004.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195]](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0002.jpg)
![Western Blot: eIF4E Antibody [NBP1-57195] - HEK293 lysate, mouse brain extract, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP1-57195-img0003.jpg)
![Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.](http://images.novusbio.com/fullsize/nbp3-21524_rabbit-eif4e-p-ser-209-mab-sr1401-5720231754887.jpg "Immunohistochemistry: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Immunohistochemical analysis of paraffin-embedded mouse kidney, using NBP3-21524.")
![Western Blot: eIF4E [p Ser 209] Antibody (SR1401) [NBP3-21524] - Western blot analysis using NBP3-21524 in (1) HEK293 cell lysate; (2) Mouse spleen lysate.](http://images.novusbio.com/fullsize/nbp3-21524_rabbit-eif4e-p-ser-209-mab-sr1401-6720239533793.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21317_rabbit-eif4e-pab-4520231553437.jpg)
![Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Western-Blot-NBP2-57098-img0002.jpg "Western Blot: eIF4E Antibody [NBP2-57098] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: eIF4E Antibody [NBP2-57098] - Staining of human cell line U-2 OS shows localization to cytosol & cytoplasmic bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF4E-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57098-img0001.jpg)
![Immunohistochemistry: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Image of eIF4E [p Ser209] Antibody (1F3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26426_rabbit-eif4e-p-ser-209-mab-1f3-262202414471518.jpg "Immunohistochemistry: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Image of eIF4E [p Ser209] Antibody (1F3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: eIF4E [p Ser209] Antibody (1F3) [NBP3-26426] - Positive Western Blot detected in 293 whole cell lysate, HepG2 whole cell lysate (treated with Calyculin A or not). All lanes: eIF4E [p Ser209] Antibody at 1.2ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 25 KDa Observed band size: 25 KDa](http://images.novusbio.com/fullsize/nbp3-26426_rabbit-eif4e-p-ser-209-mab-1f3-282202411472143.jpg)