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eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

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Immunocytochemistry/Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) followed by goat anti- Mouse IgG-CF488 (green).
Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed HeLa cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) ...read more
Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Flow cytometric analysis of PFA-fixed U87 cells. eIF4E antibody (PCRP-EIF4E-1D3) followed by goat anti-mouse IgG-CF488 (blue), isotype ...read more
Immunocytochemistry/ Immunofluorescence: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Immunofluorescence Analysis of PFA-fixed MCF-7 cells using eIF4E Mouse Monoclonal Antibody (PCRP-EIF4E-1D3) ...read more
Flow Cytometry: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Flow cytometric analysis of PFA-fixed Raji cells.eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3) followed by goat anti- Mouse ...read more
Protein Array: eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free [NBP3-08884] - Analysis of Protein Array containing more than 19,000 full-length human proteins using eIF4E Mouse Monoclonal Antibody (PCRP-eIF4E-1D3).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, MA
Clone
PCRP-EIF4E-1D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-08884AF350
Alexa Fluor 405
NBP3-08884AF405
Alexa Fluor 488
NBP3-08884AF488
Alexa Fluor 532
NBP3-08884AF532
Alexa Fluor 594
NBP3-08884AF594
Alexa Fluor 647
NBP3-08884AF647
Alexa Fluor 700
NBP3-08884AF700
Alexa Fluor 750
NBP3-08884AF750
Allophycocyanin
NBP3-08884APC
Biotin
NBP3-08884B
CoraFluor 1
NBP3-08884CL1
DyLight 350
NBP3-08884UV
DyLight 405
NBP3-08884V
DyLight 488
NBP3-08884G
DyLight 550
NBP3-08884R
DyLight 594
NBP3-08884DL594
DyLight 650
NBP3-08884C
DyLight 680
NBP3-08884FR
DyLight 755
NBP3-08884IR
FITC
NBP3-08884F
HRP
NBP3-08884H
Janelia Fluor 525
NBP3-08884JF525
Janelia Fluor 549
NBP3-08884JF549
Janelia Fluor 585
NBP3-08884JF585
Janelia Fluor 635
NBP3-08884JF635
Janelia Fluor 646
NBP3-08884JF646
Janelia Fluor 669
NBP3-08884JF669
PE
NBP3-08884PE
PerCP
NBP3-08884PCP
Unconjugated
NBP3-07141-100ug
mFluor Violet 450 SE
NBP3-08884MFV450
mFluor Violet 500 SE
NBP3-08884MFV500
mFluor Violet 610 SE
NBP3-08884MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-07141-100ug
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eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07141).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant full-length human eIF4E protein (Uniprot: P06730)
Localization
Cytoplasm
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
EIF4E
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Theoretical MW
25.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for eIF4E Antibody (PCRP-EIF4E-1D3) - Azide and BSA Free

  • CBP
  • eIF4E
  • eIF-4E
  • EIF4E1
  • EIF4EL1
  • EIF4EL1MGC111573
  • eIF-4F 25 kDa subunit
  • EIF4F
  • eukaryotic translation initiation factor 4E
  • eukaryotic translation initiation factor 4E-like 1
  • mRNA cap-binding protein

Background

eIF-4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It exists in two forms: as a free form (25 kDa) and as part of a multiprotein complex eIF-4F (1). eIF-4E appears to be the least abundant of the initiation factors and acts as a rate-limiting step of initiation (2). Since translation is regulated by phosphorylation, eIF-4E phosphorylation at Ser 209 by MAPK signal-integrating kinase 1 (Mnk1) and kinase 2 (Mnk2) may directly regulate the rate of protein synthesis initiation (3). There is also evidence that eIF-4E can function as an oncogene (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Bioinformatics

Gene Symbol EIF4E