Claudin-1 Antibodies
We offer Claudin-1 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each Claudin-1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Claudin-1 Antibodies can be used in a variety of model species: Canine, Human, Mouse, Primate, Rat. Use the list below to choose the Claudin-1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Claudin-1 polyclonal antibodies and browse our Claudin-1 monoclonal antibody catalog.
Alternate Names for Claudin-1 Antibodies
anti-Claudin-1 antibody, anti-CLDN1 antibody, anti-claudin 1 antibody, anti-CLD1 antibody, anti-ILVASCSenescence-associated epithelial membrane protein antibody, anti-SEMP1senescence-associated epithelial membrane protein 1 antibody
11 Results for "claudin-1" in Primary Antibodies
![Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-Western-Blot-H00009076-M01-img0015.jpg "Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in A-431.")
![Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-Immunohistochemistry-Paraffin-H00009076-M01-img0011.jpg)
![Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - CLDN1 monoclonal antibody (M01), clone 1C5-D9. Analysis of CLDN1 expression in PRC/PRF/5.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-Western-Blot-H00009076-M01-img0014.jpg)
![Western Blot: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-Western-Blot-H00009076-M01-img0016.jpg)
![Immunohistochemistry-Paraffin: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-Immunohistochemistry-Paraffin-H00009076-M01-img0009.jpg)
![Immunoprecipitation: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Analysis of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-Immunoprecipitation-H00009076-M01-img0012.jpg)
![ELISA: Claudin-1 Antibody (1C5-D9) [H00009076-M01] - Detection limit for recombinant GST tagged CLDN1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-1C5-D9-ELISA-H00009076-M01-img0017.jpg)
![Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. The relative expression of claudin-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Western-Blot-NBP1-77036-img0012.jpg "<b>Biological Strategies Validation. </b>Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Effects of dietary Flammulina velutipes stem waste (FVS) inclusion on the relative expression of tight junction proteins in jejunum of growing pigs. The relative expression of claudin-1 in jejunum of growing pigs fed control and 2.5% or 5% FVS diets. Values are means (3 pigs per treatment) with standard errors represented by vertical bars. *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following publication (//jasbsci.biomedcentral.com/articles/10.1186/s40104-020-00449-y) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation of CLDN1 in HepG2 Cells. Analysisof 4% paraformaldehyde-fixed HepG2 cells labeling CLDN1 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77036-img0006.jpg)
![Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane.Antibodies: CLDN1, (1 ug/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Western-Blot-NBP1-77036-img0007.jpg)
![Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human Tissue Lysates Loading: 15 ug of lysates per lane.Antibodies: CLDN1,(1 ug/mL) , 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Western-Blot-NBP1-77036-img0008.jpg)
![Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Validation in Human HepG2 Cell Lysate Loading: 15 ug of lysates per lane.Antibodies: CLDN1, 5187 (A: 1 gu/mL and B: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Western-Blot-NBP1-77036-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: CLDN1, NBP1-77036 (1 ug/mL), CLDN1, (2 ug/mL), and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-NBP1-77036-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Claudin-1 labeled pig cultured cornea cells visualized with Alexa488. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunocytochemistry-NBP1-77036-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Claudin-1 Antibody - BSA Free [NBP1-77036] - Claudin-1 Antibody [NBP1-77036] - Claudin-1 labeled pig Sc cells visualized with Alexa488. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunocytochemistry-NBP1-77036-img0005.jpg)
![Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining in human skin and skeletal muscle tissues using NBP2-38578 antibody. Corresponding CLDN1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38578-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining in human skin and skeletal muscle tissues using NBP2-38578 antibody. Corresponding CLDN1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Claudin-1 Antibody [NBP2-38578] - Analysis in human cell lines A-431 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Western-Blot-NBP2-38578-img0006.jpg)
![Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human fallopian tube shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38578-img0007.jpg)
![Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38578-img0008.jpg)
![Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38578-img0009.jpg)
![Immunohistochemistry-Paraffin: Claudin-1 Antibody [NBP2-38578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-38578-img0010.jpg)
![Western Blot: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Western blot analysis of Claudin-1 on different lysates with Rabbit anti-Claudin-1 antibody (NBP3-32189) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 ug/Lane Lane 2: HepG2 cell lysate, 10 ug/Lane Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32189) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32189_rabbit-claudin-1-mab-ps01-43-278202416285295.jpg "Western Blot: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Western blot analysis of Claudin-1 on different lysates with Rabbit anti-Claudin-1 antibody (NBP3-32189) at 1/1,000 dilution. Lane 1: A431 cell lysate, 10 ug/Lane Lane 2: HepG2 cell lysate, 10 ug/Lane Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32189) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling Claudin-1 with Rabbit anti-Claudin-1 antibody (NBP3-32189) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32189, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32189_rabbit-claudin-1-mab-ps01-43-298202413585579.jpg)
![Immunohistochemistry: Claudin-1 Antibody (PS01-43) [NBP3-32189] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Claudin-1 antibody (NBP3-32189) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32189) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32189_rabbit-claudin-1-mab-ps01-43-298202414304134.jpg)
![Western Blot: Claudin-1 Antibody (JE30-33) [NBP3-32190] - Western blot analysis of Claudin-1 on different lysates with Rabbit anti-Claudin-1 antibody (NBP3-32190) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HUVEC cell lysate (20 ug/Lane) Lane 3: Caco-2 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (negative) (20 ug/Lane) Lane 5: Mouse skin tissue lysate (40 ug/Lane) Lane 6: Mouse liver tissue lysate (40 ug/Lane) Lane 7: Rat skin tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 19 kDa Exposure time: Lane 1-2: 8 seconds; Lane 3-8: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32190) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32190_rabbit-claudin-1-mab-je30-33-278202415242672.jpg "Western Blot: Claudin-1 Antibody (JE30-33) [NBP3-32190] - Western blot analysis of Claudin-1 on different lysates with Rabbit anti-Claudin-1 antibody (NBP3-32190) at 1/2,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HUVEC cell lysate (20 ug/Lane) Lane 3: Caco-2 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (negative) (20 ug/Lane) Lane 5: Mouse skin tissue lysate (40 ug/Lane) Lane 6: Mouse liver tissue lysate (40 ug/Lane) Lane 7: Rat skin tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 19 kDa Exposure time: Lane 1-2: 8 seconds; Lane 3-8: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32190) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Claudin-1 Antibody (JE30-33) [NBP3-32190] - Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue with Rabbit anti-Claudin-1 antibody (NBP3-32190) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32190) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32190_rabbit-claudin-1-mab-je30-33-298202414304110.jpg)
![Western Blot: Claudin 1 Antibody [NBP1-59107] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Western-Blot-NBP1-59107-img0001.jpg "Western Blot: Claudin 1 Antibody [NBP1-59107] - HepG2 cell lysate, Antibody Titration: 0.2-1 ug/ml")
![Immunohistochemistry-Paraffin: Claudin 1 Antibody [NBP1-59107] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59107-img0003.jpg)
![Western Blot: Claudin-1 Antibody (4L4Q2) [NBP3-15397] - Western blot analysis of extracts of various cell lines, using Claudin-1 Rabbit mAb (NBP3-15397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Claudin-1-Antibody-4L4Q2-Western-Blot-NBP3-15397-img0001.jpg "Western Blot: Claudin-1 Antibody (4L4Q2) [NBP3-15397] - Western blot analysis of extracts of various cell lines, using Claudin-1 Rabbit mAb (NBP3-15397) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Claudin-1 Antibody (SR1308) [NBP3-21736] - Western blot analysis using NBP3-21736 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21736_rabbit-claudin-1-mab-sr1308-57202315521830.jpg "Western Blot: Claudin-1 Antibody (SR1308) [NBP3-21736] - Western blot analysis using NBP3-21736 in A431 cell lysate.")
![Western Blot: Claudin-1 Antibody (CL3698) [NBP2-61630]-Analysis in human cell lines RT-4 and U-251MG using Anti-CLDN1 antibody. Corresponding CLDN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/antibody/nbp2-61630_mouse-monoclonal-claudin-1-antibody-cl3698-western-blot-159202314508..jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Claudin-1 Antibody (CL3698) [NBP2-61630]-Analysis in human cell lines RT-4 and U-251MG using Anti-CLDN1 antibody. Corresponding CLDN1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.")
![Immunohistochemistry-Paraffin: Claudin-1 Antibody [NB120-15098] - Formalin fixed paraffin embedded human skin stained with Claudin 1 antibody (NB120-15098).](http://images.novusbio.com/fullsize/Claudin-1-Antibody-Immunohistochemistry-Paraffin-NB120-15098-img0002.jpg "Immunohistochemistry-Paraffin: Claudin-1 Antibody [NB120-15098] - Formalin fixed paraffin embedded human skin stained with Claudin 1 antibody (NB120-15098).")