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CKAP4/p63 Antibodies

We offer CKAP4/p63 Antibodies for use in common research applications: Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each CKAP4/p63 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our CKAP4/p63 Antibodies can be used in a variety of model species: Human, Mammal, Mouse, Rat. Use the list below to choose the CKAP4/p63 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our CKAP4/p63 polyclonal antibodies.

Alternate Names for CKAP4/p63 Antibodies

anti-CKAP4/p63 antibody, anti-CKAP4 antibody, anti-63 kDa membrane protein antibody, anti-CLIMP-63 antibody, anti-cytoskeleton-associated protein 4 antibody, anti-ERGIC-63 antibody, anti-MGC99554 antibody, anti-P63 antibody, anti-transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediatecompartment antibody, anti-type-II transmembrane protein p63 antibody

9 Results for "ckap4-p63" in Primary Antibodies

MAB11600

CKAP4/p63 Antibody (1086526) [Unconjugated]

100 μg

MAB11600

Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human CKAP4/p63 Monoclonal Antibody (Catalog # MAB11600) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CKAP4/p63 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

CKAP4/p63 was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human CKAP4/p63 Monoclonal Antibody (Catalog # MAB11600) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CKAP4/p63 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human CKAP4/p63 Monoclonal Antibody (Catalog # MAB11600) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CKAP4/p63 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CKAP4/p63 Monoclonal Antibody (Catalog # MAB11600) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

CKAP4/p63 was detected in immersion fixed HT-1080 cells and MG‑63 human osteosarcoma cell line using Mouse Anti-Human CKAP4/p63 Monoclonal Antibody (Catalog # MAB11600) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the endoplasmic reticulum membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/ml. A specific band was detected for CKAP4/p63 at approximately 65 kDa (as indicated) using 4 µg/mL of Mouse Anti-Human CKAP4/p63 Monoclonal Antibody (Catalog # MAB11600). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11600-SP

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AF7355

CKAP4/p63 Antibody [Unconjugated]

(2 Publications)

100 μg

AF7355

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line, TK-6 human lymphoblast cell line, and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CKAP4/p63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7355) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for CKAP4/p63 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

CKAP4/p63 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human CKAP4/p63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7355) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

CKAP4/p63 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Sheep Anti-Human CKAP4/p63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7355) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; NL012). Cytokeratin 14 was also detected using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (MAB3164) and stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007). Cells were counterstained with DAPI (blue). Specific staining of CKAP4/p63 was localized to perinuclear space and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/ml. A specific band was detected for CKAP4/p63 at approximately 65 kDa (as indicated) using 4 µg/mL of Sheep Anti-Human CKAP4/p63 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7355). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7355-SP

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NBP1-85571

CKAP4/p63 Antibody

0.1 ml

NBP1-85571

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining in human cervix, uterine and skeletal muscle tissues using anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human bone marrow, cerebral cortex, cervix, uterine and skeletal muscle using Anti-CKAP4 antibody NBP1-85571 (A) shows similar protein distribution across tissues to independent antibody NBP1-85572 (B).

<b>Orthogonal Strategies Validation. </b>Western Blot: CKAP4/p63 Antibody [NBP1-85571] - Analysis in human cell lines U-251MG and MCF-7 using Anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human skeletal muscle using Anti-CKAP4 antibody NBP1-85571.

Western Blot: CKAP4/p63 Antibody [NBP1-85571] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human cervix, uterine shows high expression.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human bone marrow using Anti-CKAP4 antibody.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human cerebral cortex using Anti-CKAP4 antibody.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human cervix, uterine using Anti-CKAP4 antibody NBP1-85571.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-85572

CKAP4/p63 Antibody

(1 Publication)

0.1 ml

NBP1-85572

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining in human cervix, uterine and skeletal muscle tissues using anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same tissues.

<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human bone marrow, cerebral cortex, cervix, uterine and skeletal muscle using Anti-CKAP4 antibody NBP1-85572 (A) shows similar protein distribution across tissues to independent antibody NBP1-85571 (B).

<b>Independent Antibodies Validation. </b>Western Blot: CKAP4/p63 Antibody [NBP1-85572] - Analysis using Anti-CKAP4 antibody NBP1-85572 (A) shows similar pattern to independent antibody NBP1-85571 (B).

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human skeletal muscle shows low expression as expected.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human bone marrow using Anti-CKAP4 antibody.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human cervix, uterine using Anti-CKAP4 antibody NBP1-85572.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human skeletal muscle using Anti-CKAP4 antibody NBP1-85572.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human cervix, uterine shows high expression.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-15908

CKAP4/p63 Antibody

0.1 ml

NBP2-15908

Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP2-15908] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 antibody [N3C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - Human colon cancer. CKAP4 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CL1-5 xenograft, using CKAP4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CKAP4/p63 antibody [N3C3] detects CKAP4/p63 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.CKAP4/p63 stained by CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-26643

CKAP4/p63 Antibody

(1 Review)

(2 Publications)

0.1 ml

NBP1-26643

Western Blot: CKAP4/p63 Antibody [NBP1-26643] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641 and NBP1-26642.

Reactivity:

Hu, Mu, Ma

Species Glossary

Reactivity: Hu, Mu, Ma

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-26642

CKAP4/p63 Antibody

(1 Publication)

0.1 ml

NBP1-26642

Western Blot: CKAP4/p63 Antibody [NBP1-26642] - Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26641and NBP1-26643.

<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-26642] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-55266

CKAP4/p63 Antibody

100 ul

NBP2-55266

Western Blot: CKAP4/p63 Antibody [NBP2-55266] - Analysis in human cell line U-251 MG.

Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP2-55266] - Staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.

<b>Independent Antibodies Validation. </b>Western Blot: CKAP4/p63 Antibody [NBP2-55266] - Analysis using Anti-CKAP4 antibody NBP2-55266 (A) shows similar pattern to independent antibody NBP1-85572 (B).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-26641

CKAP4/p63 Antibody

0.1 ml

NBP1-26641

Western Blot: CKAP4/p63 Antibody [NBP1-26641] - CKAP4 Antibody [NBP1-26641] - Whole cell lysate from HeLa and 293T cells. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies NBP1-26642 and NBP1-26643.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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