Alpha Actinin 4 Antibodies
We offer Alpha Actinin 4 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Sandwich ELISA, Western Blot. Each Alpha Actinin 4 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Alpha Actinin 4 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the Alpha Actinin 4 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Alpha Actinin 4 polyclonal antibodies and browse our Alpha Actinin 4 monoclonal antibody catalog.
Alternate Names for Alpha Actinin 4 Antibodies
anti-Alpha Actinin 4 antibody, anti-ACTN4 antibody, anti-actinin alpha4 isoform antibody, anti-actinin, alpha 4 antibody, anti-ACTININ-4 antibody, anti-alpha-actinin-4 antibody, anti-DKFZp686K23158 antibody, anti-F-actin cross-linking protein antibody, anti-focal segmental glomerulosclerosis 1 antibody, anti-FSGS antibody, anti-FSGS1 antibody, anti-Non-muscle alpha-actinin 4 antibody
15 Results for "alpha-actinin-4" in Primary Antibodies
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.Lane 1: ACTN4 transfected lysate(104.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0021.jpg "Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.Lane 1: ACTN4 transfected lysate(104.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on HeLa cell. Antibody concentration 20 ug/ml.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Immunocytochemistry-Immunofluorescence-H00000081-M01-img0010.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human lung adenocarcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Immunohistochemistry-Paraffin-H00000081-M01-img0014.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in PC-12.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0016.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10 Analysis of ACTN4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0017.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in Raw 264.7.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0018.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in MCF-7.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0019.jpg)
![Western Blot: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - ACTN4 monoclonal antibody (M01), clone 4D10. Analysis of ACTN4 expression in NIH/3T3.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Western-Blot-H00000081-M01-img0020.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Analysis of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-Immunohistochemistry-Paraffin-H00000081-M01-img0012.jpg)
![ELISA: Alpha Actinin 4 Antibody (4D10) [H00000081-M01] - Detection limit for recombinant GST tagged ACTN4 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-4D10-ELISA-H00000081-M01-img0015.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Alpha Actinin 4 antibody detects ACTN4 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 7.5% SDS-PAGE. Alpha Actinin 4 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15357-img0012.jpg "Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Alpha Actinin 4 antibody detects ACTN4 protein by western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole cell lysate/extract. F. 30 ug Raw264.7 whole cell lysate/extract. G. 30 ug C2C12 whole cell lysate/extract. 7.5% SDS-PAGE. Alpha Actinin 4 antibody dilution: 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15357] - Paraformaldehyde-fixed HeLa, using alpha Actinin 4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15357-img0001.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Mouse intestine. alpha Actinin 4 stained by alpha Actinin 4 antibody [C2C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-15357-img0013.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15357-img0003.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15357-img0005.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15357] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract7.5 % SDS-PAGE dilution: 1:1000](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15357-img0011.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - A549 xenograft, using alpha Actinin 4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-15357-img0002.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP2-15357] - Mouse intestine. alpha Actinin 4 antibody [C2C3], C-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-15357-img0007.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP1-89818] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP1-89818-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Alpha Actinin 4 Antibody [NBP1-89818] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89818-img0008.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-89818-img0001.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP1-89818] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP1-89818-img0007.jpg)
![Western Blot: Alpha Actinin 4 Antibody (7H6) [NB100-74352] - Analysis of alpha actinin 4 antibody.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-7H6-Western-Blot-NB100-74352-img0005.jpg "Western Blot: Alpha Actinin 4 Antibody (7H6) [NB100-74352] - Analysis of alpha actinin 4 antibody.")
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (7H6) [NB100-74352] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-7H6-Immunohistochemistry-Paraffin-NB100-74352-img0008.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (7H6) [NB100-74352] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-7H6-Immunohistochemistry-Paraffin-NB100-74352-img0006.jpg)
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (7H6) [NB100-74352] - Both normal and cancer biopsies of deparaffinized Human kidney tissues.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-7H6-Immunohistochemistry-Paraffin-NB100-74352-img0007.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - Alpha Actinin 4 antibody detects alpha Actinin 4 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 4 antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15358-img0005.jpg "Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - Alpha Actinin 4 antibody detects alpha Actinin 4 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Alpha Actinin 4 antibody diluted by 1:1000.")
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP2-15358] - Sample: MCF-7 cells were fixed in iced-cold MeOH for 5 min. Green: ACTN4 protein stained by alpha Actinin 4 antibody, N-term diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15358-img0001.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15358-img0002.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - 30 ug PC-12 whole cell lysate/extract 7. 5% SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15358-img0003.jpg)
![Western Blot: Alpha Actinin 4 Antibody [NBP2-15358] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Western-Blot-NBP2-15358-img0004.jpg)
![Western Blot: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Western blot analysis of extracts of various cell lines, using Alpha Actinin 4 Rabbit mAb (NBP3-16176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-5Y3R6-Western-Blot-NBP3-16176-img0004.jpg "Western Blot: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Western blot analysis of extracts of various cell lines, using Alpha Actinin 4 Rabbit mAb (NBP3-16176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Immunofluorescence analysis of U-2 OS cells using Alpha Actinin 4 Rabbit mAb (NBP3-16176) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-5Y3R6-Immunocytochemistry-Immunofluorescence-NBP3-16176-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Immunofluorescence analysis of C6 cells using Alpha Actinin 4 Rabbit mAb (NBP3-16176) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-5Y3R6-Immunocytochemistry-Immunofluorescence-NBP3-16176-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Immunofluorescence analysis of NIH-3T3 cells using Alpha Actinin 4 Rabbit mAb (NBP3-16176) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-5Y3R6-Immunocytochemistry-Immunofluorescence-NBP3-16176-img0002.jpg)
![Immunoprecipitation: Alpha Actinin 4 Antibody (5Y3R6) [NBP3-16176] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug Alpha Actinin 4 antibody (NBP3-16176). Western blot was performed from the immunoprecipitate using Alpha Actinin 4 antibody (NBP3-16176) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-5Y3R6-Immunoprecipitation-NBP3-16176-img0005.jpg)
![Western Blot: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888] - Western blot detection of Alpha Actinin 4 in K562, Rat Brain, C6, 3T3, Hela cell lysates using Alpha Actinin 4 Rabbit mAb (1:1000 diluted). Predicted band size: 105kDa. Observed band size: 105kDa.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-S06-3D7-Western-Blot-NBP3-14888-img0001.jpg "Western Blot: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888] - Western blot detection of Alpha Actinin 4 in K562, Rat Brain, C6, 3T3, Hela cell lysates using Alpha Actinin 4 Rabbit mAb (1:1000 diluted). Predicted band size: 105kDa. Observed band size: 105kDa.")
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody (S06-3D7) [NBP3-14888] - Immunofluorescence of Alpha Actinin 4 (green) in Hela cells using Alpha Actinin 4 Rabbit mAb at dilution 1/200, and DAPI (blue)](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-S06-3D7-Immunocytochemistry-Immunofluorescence-NBP3-14888-img0002.jpg)
![Western Blot: Alpha Actinin 4 Antibody (RM287) [NBP2-61485] - Western Blot of HeLa cells lysates using NBP2-61485 at a 1:1000 dilution.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-RM287-Western-Blot-NBP2-61485-img0003.jpg "Western Blot: Alpha Actinin 4 Antibody (RM287) [NBP2-61485] - Western Blot of HeLa cells lysates using NBP2-61485 at a 1:1000 dilution.")
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody (RM287) [NBP2-61485] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using NBP2-61485 at a 1:200 dilution](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-RM287-Immunohistochemistry-Paraffin-NBP2-61485-img0002.jpg)
![Western Blot: Alpha Actinin 4 [p Tyr4] Antibody [NBP3-23333] - Western blot analysis of Alpha Actinin 4 in A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1,2,5.6) or after immunoprecipitation using Alpha Actinin 4 (C-terminal region) antibody in the absence (lanes 3 & 7) or presence of pervanadate-treated A431 cell lysate (lanes 4 & 8). Some lanes of the blot were treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-Alpha Actinin 4 (C-terminal region) or anti-Alpha Actinin 4 (Tyr-4).](http://images.novusbio.com/fullsize/nbp3-23333_rabbit-alpha-actinin-4-p-tyr4-pab-11920231665312.jpg "Western Blot: Alpha Actinin 4 [p Tyr4] Antibody [NBP3-23333] - Western blot analysis of Alpha Actinin 4 in A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1,2,5.6) or after immunoprecipitation using Alpha Actinin 4 (C-terminal region) antibody in the absence (lanes 3 & 7) or presence of pervanadate-treated A431 cell lysate (lanes 4 & 8). Some lanes of the blot were treated with alkaline phosphatase (lanes 2 & 6). The blots were probed with anti-Alpha Actinin 4 (C-terminal region) or anti-Alpha Actinin 4 (Tyr-4).")
![Western Blot: Alpha Actinin 4 Antibody [NBP3-23332] - Western blot analysis of Alpha Actinin 4 in human A431 cells (lanes 1 & 3) and rabbit spleen fibroblasts (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Alpha Actinin 4 4 (a.a. 2-11) or mouse monoclonal anti-Alpha Actinin 4 (C-terminal region).](http://images.novusbio.com/fullsize/nbp3-23332_rabbit-alpha-actinin-4-pab-1192023166530.jpg "Western Blot: Alpha Actinin 4 Antibody [NBP3-23332] - Western blot analysis of Alpha Actinin 4 in human A431 cells (lanes 1 & 3) and rabbit spleen fibroblasts (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Alpha Actinin 4 4 (a.a. 2-11) or mouse monoclonal anti-Alpha Actinin 4 (C-terminal region).")
![Western Blot: Alpha Actinin 4 Antibody (8G12) [H00000081-M06] - Detection against Immunogen (131.3 KDa) .](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-8G12-Western-Blot-H00000081-M06-img0001.jpg "Western Blot: Alpha Actinin 4 Antibody (8G12) [H00000081-M06] - Detection against Immunogen (131.3 KDa) .")
![ELISA: Alpha Actinin 4 Antibody (1E10) [H00000081-M02] - Detection limit for recombinant GST tagged ACTN4 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-1E10-ELISA-H00000081-M02-img0001.jpg "ELISA: Alpha Actinin 4 Antibody (1E10) [H00000081-M02] - Detection limit for recombinant GST tagged ACTN4 is 0.3 ng/ml as a capture antibody.")