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![Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Western-Blot-NB300-549-img0008.jpg "Western Blot: Adenosine A1R Antibody [NB300-549] - Analysis was performed on whole cell extracts (30 ug lysate) of PC 12 (Lane 1), SH-SY5Y (lane 2), U-87MG (lane 3), A549 (Lane 4) and K-562 (lane 5).")
![Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on 70% confluent log phase HeLa cells.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-549-img0007.jpg)
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NB300-549] - Analysis showing positive staining in the cytoplasm of Rat brain tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunohistochemistry-Paraffin-NB300-549-img0003.jpg)
![Flow Cytometry: Adenosine A1R Antibody [NB300-549] - Analysis of Adenosine Receptor A1 was done on A549 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Adenosine Receptor A1 Rabbit Polyclonal Antibody (PA1041A, red histogram) or with rabbit isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Flow-Cytometry-NB300-549-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NB300-549] - Dissociated rat hippocampal culture. Image courtesy of Janosch Heller, University College London.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunocytochemistry-Immunofluorescence-NB300-549-img0002.jpg)
![Western Blot: Adenosine A1R Antibody (8C3P3) [NBP3-16594] - Western blot analysis of extracts of various cell lines, using Adenosine A1R Rabbit mAb (NBP3-16594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-8C3P3-Western-Blot-NBP3-16594-img0004.jpg "Western Blot: Adenosine A1R Antibody (8C3P3) [NBP3-16594] - Western blot analysis of extracts of various cell lines, using Adenosine A1R Rabbit mAb (NBP3-16594) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody (8C3P3) [NBP3-16594] - Immunohistochemistry of paraffin-embedded mouse spinal cord using Adenosine A1R Rabbit mAb (NBP3-16594) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-8C3P3-Immunohistochemistry-Paraffin-NBP3-16594-img0003.jpg)
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody (8C3P3) [NBP3-16594] - Immunohistochemistry of paraffin-embedded rat brain using Adenosine A1R Rabbit mAb (NBP3-16594) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-8C3P3-Immunohistochemistry-Paraffin-NBP3-16594-img0001.jpg)
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody (8C3P3) [NBP3-16594] - Immunohistochemistry of paraffin-embedded human brain using Adenosine A1R Rabbit mAb (NBP3-16594) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-8C3P3-Immunohistochemistry-Paraffin-NBP3-16594-img0002.jpg)
![Western Blot: Adenosine A1R Antibody [NBP3-15570] - Western blot analysis of extracts of Mouse heart, using Adenosine A1R antibody (NBP3-15570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Western-Blot-NBP3-15570-img0003.jpg "Western Blot: Adenosine A1R Antibody [NBP3-15570] - Western blot analysis of extracts of Mouse heart, using Adenosine A1R antibody (NBP3-15570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NBP3-15570] - Immunohistochemistry of paraffin-embedded human esophageal using Adenosine A1R Rabbit pAb (NBP3-15570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Immunohistochemistry-Paraffin-NBP3-15570-img0002.jpg)
![Immunohistochemistry-Paraffin: Adenosine A1R Antibody [NBP3-15570] - Immunohistochemistry of paraffin-embedded rat brain using Adenosine A1R Rabbit pAb (NBP3-15570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Immunohistochemistry-Paraffin-NBP3-15570-img0001.jpg)
![Western Blot: Adenosine A1R Antibody (SR1610) [NBP3-21593] - Western blot analysis using NBP3-21593 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21593_rabbit-adenosine-a1r-mab-sr1610-57202317161842.jpg "Western Blot: Adenosine A1R Antibody (SR1610) [NBP3-21593] - Western blot analysis using NBP3-21593 in A549 cell lysate.")
![Western Blot: Adenosine A1R Antibody (SR1610) [NBP3-21593] - Western blot analysis of Adenosine A1R expression in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21593_rabbit-adenosine-a1r-mab-sr1610-23420241452104.jpg)
![Immunohistochemistry: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32002_rabbit-adenosine-a1r-mab-je31-35-298202413585567.jpg "Immunohistochemistry: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32002) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: Adenosine A1R Antibody (JE31-35) [NBP3-32002] - Western blot analysis of Adenosine A1R on different lysates with Rabbit anti-Adenosine A1R antibody (NBP3-32002) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 ug/Lane) Lane 2: HEK-293 cell lysate (15 ug/Lane) Lane 3: U-87 MG cell lysate (15 ug/Lane) Lane 4: SH-SY5Y cell lysate (15 ug/Lane) Lane 5: HeLa cell lysate (15 ug/Lane) Lane 6: Raji cell lysate (15 ug/Lane) Lane 7: NIH/3T3 cell lysate (15 ug/Lane) Lane 8: Human brain tissue lysate (30 ug/Lane) Lane 9: Mouse brain tissue lysate (30 ug/Lane) Lane 10: Rat brain tissue lysate (30 ug/Lane) Lane 11: Human cerebellum tissue lysate (30 ug/Lane) Lane 12: Rat cerebellum tissue lysate (30 ug/Lane) Predicted band size: 36.5 kDa Observed band size: 36.5/40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32002) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32002_rabbit-adenosine-a1r-mab-je31-35-392024845353.jpg)
![Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218] - Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate. All lanes: Adenosine A1R antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37, 14 KDa. Observed band size: 37 KDa.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-10D12-Western-Blot-NBP3-15218-img0002.jpg "Western Blot: Adenosine A1R Antibody (10D12) [NBP3-15218] - Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate. All lanes: Adenosine A1R antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 37, 14 KDa. Observed band size: 37 KDa.")
![Immunohistochemistry: Adenosine A1R Antibody (10D12) [NBP3-15218] - IHC image of Adenosine A1R antibody diluted at 1:100 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-10D12-Immunohistochemistry-NBP3-15218-img0001.jpg)
![Western Blot: Adenosine A1R Antibody [NBP2-87922] - Staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Western-Blot-NBP2-87922-img0001.jpg "Western Blot: Adenosine A1R Antibody [NBP2-87922] - Staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Flow Cytometry: Adenosine A1R Antibody [NBP2-87922] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Adenosine-A1R-Antibody-Flow-Cytometry-NBP2-87922-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NBP2-58443] - Staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58443-img0001.jpg "Immunocytochemistry/Immunofluorescence: Adenosine A1R Antibody [NBP2-58443] - Staining of human cell line U-2 OS shows localization to plasma membrane.")
![Immunohistochemistry-Paraffin: Adenosine A1 R Antibody [NLS11] - Analysis of anti-Adenosine A1 Receptor antibody of rat brain, Purkinje neurons.](http://images.novusbio.com/fullsize/Adenosine-A1-R-Antibody-Immunohistochemistry-Paraffin-NLS11-img0003.jpg "Immunohistochemistry-Paraffin: Adenosine A1 R Antibody [NLS11] - Analysis of anti-Adenosine A1 Receptor antibody of rat brain, Purkinje neurons.")