ABCF1 Antibodies
We offer ABCF1 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Immunoprecipitation (Negative), Sandwich ELISA, Western Blot. Each ABCF1 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our ABCF1 Antibodies can be used in a variety of model species: Human. Use the list below to choose the ABCF1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our ABCF1 polyclonal antibodies and browse our ABCF1 monoclonal antibody catalog.
Alternate Names for ABCF1 Antibodies
anti-ABCF1 antibody, anti-ABC27 antibody, anti-ABC50TNFalpha-inducible ATP-binding protein antibody, anti-ATP-binding cassette 50 (TNF-alpha stimulated) antibody, anti-ATP-binding cassette 50 antibody, anti-ATP-binding cassette sub-family F member 1 antibody, anti-ATP-binding cassette, sub-family F (GCN20), member 1 antibody, anti-EC 2.7.7.6 antibody, anti-EC 3.6.3.17 antibody, anti-EST123147 antibody, anti-TNF-alpha-stimulated ABC protein antibody
6 Results for "abcf1" in Primary Antibodies
![Western Blot: ABCF1 Antibody [NBP1-89314] - Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-89314-img0014.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCF1 Antibody [NBP1-89314] - Western blot analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ABCF1 Antibody [NBP1-89314] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89314-img0006.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ABCF1 Antibody [NBP1-89314] - Analysis in human cell lines Caco-2 and MCF-7 using anti-ABCF1 antibody. Corresponding ABCF1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-89314-img0009.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0010.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0011.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-89314] - Staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-89314-img0013.jpg)
![Western Blot: ABCF1 Antibody [NBP1-18889] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-18889 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABC50 was also immunoprecipitated by NBP1-18888, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-18889-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ABCF1 Antibody [NBP1-18889] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NBP1-18889 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ABC50 was also immunoprecipitated by NBP1-18888, which recognizes an upstream epitope.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: ABCF1 Antibody [NBP1-18889] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ABC50 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-NBP1-18889-img0007.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP1-18889] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-ABC50 NBP1-18889 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP1-18889-img0004.jpg)
![Western Blot: ABCF1 Antibody [NBP2-15226] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP2-15226-img0003.jpg "Western Blot: ABCF1 Antibody [NBP2-15226] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE, antibody diluted at 1:3000.")
![Immunocytochemistry/Immunofluorescence: ABCF1 Antibody [NBP2-15226] - Paraformaldehyde-fixed HeLa, using ABCF1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15226-img0001.jpg)
![Immunohistochemistry-Paraffin: ABCF1 Antibody [NBP2-15226] - Paraffin-embedded Hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-Paraffin-NBP2-15226-img0002.jpg)
![Western Blot: ABCF1 Antibody [NBP1-18888] - AWhole cell lysate from HeLa and 293T cells. ABC50 was also immunoprecipitated by another rabbit anti-ABC50 antibody.](http://images.novusbio.com/fullsize/ABCF1-Antibody-Western-Blot-NBP1-18888-img0005.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ABCF1 Antibody [NBP1-18888] - AWhole cell lysate from HeLa and 293T cells. ABC50 was also immunoprecipitated by another rabbit anti-ABC50 antibody.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: ABCF1 Antibody [NBP1-18888] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ABC50 used at a dilution of 1:200 ( 1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit .](http://images.novusbio.com/fullsize/ABCF1-Antibody-Immunohistochemistry-NBP1-18888-img0006.jpg)
![Western Blot: ABCF1 Antibody (4P9V8) [NBP3-15819] - Western blot analysis of extracts of various cell lines, using ABCF1 Rabbit mAb (NBP3-15819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ABCF1-Antibody-4P9V8-Western-Blot-NBP3-15819-img0002.jpg "Western Blot: ABCF1 Antibody (4P9V8) [NBP3-15819] - Western blot analysis of extracts of various cell lines, using ABCF1 Rabbit mAb (NBP3-15819) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: ABCF1 Antibody (4P9V8) [NBP3-15819] - Immunohistochemistry of paraffin-embedded human colon using ABCF1 Rabbit mAb (NBP3-15819) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ABCF1-Antibody-4P9V8-Immunohistochemistry-Paraffin-NBP3-15819-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ABCF1 Antibody (1B4) [H00000023-M01] - Analysis of monoclonal antibody to ABCF1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ABCF1-Antibody-1B4-Immunocytochemistry-Immunofluorescence-H00000023-M01-img0002.jpg "Immunocytochemistry/Immunofluorescence: ABCF1 Antibody (1B4) [H00000023-M01] - Analysis of monoclonal antibody to ABCF1 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: ABCF1 Antibody (1B4) [H00000023-M01] - Detection limit for recombinant GST tagged ABCF1 is 3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ABCF1-Antibody-1B4-ELISA-H00000023-M01-img0003.jpg)