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Methotrexate Transport Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Methotrexate Transport Pathway and Leukemia L1210, Leukemia, Neoplasms, Malignant Neoplasms, Acute Lymphocytic Leukemia. The study of the Methotrexate Transport Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Methotrexate Transport Pathway has been researched in relation to Transport, Drug Resistance, Localization, Excretion, Translation. The Methotrexate Transport Pathway complements our catalog of research reagents including antibodies and ELISA kits against MTX1, DHFR, SLC19A1, RFC1, ABCC2.

Top Research Reagents

We have 1915 products for the study of the Methotrexate Transport Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

Dihydrofolate Reductase/DHFR Antibody (2B10)
H00001719-M01
Western Blot: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Folic acid treatment restores endothelial specific DHFR expression and activity in Ang II-infused apoE null mice.DHFR expression (A, n?=?8-11) and activity (B, n?=?5-7) measured from endothelial cells (ECs) and EC-denuded aortas isolated from apoE null mice after 4 weeks of infusion. Data show that DHFR expression and activity was restored in FA treated animals specifically in the endothelial cells. eNOS was stained to show that isolation/removal of ECs from the vessels was successful. *p<0.05. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0088899) licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Dihydrofolate Reductase/DHFR Antibody (2B10) [H00001719-M01] - Analysis of monoclonal antibody to DHFR on HeLa cell. Antibody concentration 10 ug/ml.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

12 Publications
MSH3 Antibody (CL14059)
NBP3-24587
Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.Western blot analysis in human cell line U-251 MG.

Mouse Monoclonal
Species Human
Applications WB, ICC/IF, IHC

Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free
NBP3-25721
Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Reduced Folate Carrier/SLC19A1 Antibody
NBP1-59904
Western Blot: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-59904] - Titration: 5.0ug/ml Positive Control: Jurkat cell lysate.Immunohistochemistry-Paraffin: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-59904] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
MRP2 Antibody
NBP1-69023
Western Blot: MRP2 Antibody [NBP1-69023] - Sample Tissue: Mouse Thymus Antibody Dilution: 1.0ug/mlWestern Blot: MRP2 Antibody [NBP1-69023] - Mouse Spleen lysate, concentration 0.2-1 ug/ml.

Rabbit Polyclonal
Species Mouse
Applications WB

4 Publications
KLHL1 Antibody - BSA Free
NBP1-76670
Western Blot: KLHL1 Antibody [NBP1-76670] - Analysis of KLHL1 in HeLa cell lysate with KLHL1 antibody at (A) 2 and (B) 4 ug/mL.Immunohistochemistry-Paraffin: KLHL1 Antibody [NBP1-76670] - Rat brain tissue with KLHL1 antibody at 10 ug/ml.

Rabbit Polyclonal
Species Human, Rat
Applications WB, ELISA, IHC

Metaxin 1 Antibody
NBP1-87741
Western Blot: Metaxin 1 Antibody [NBP1-87741] - Analysis in human cell line MCF-7.Immunocytochemistry/Immunofluorescence: Metaxin 1 Antibody [NBP1-87741] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

SLC22A8 Antibody
NBP1-92396
Immunohistochemistry-Paraffin: SLC22A8 Antibody [NBP1-92396] - Analysis in human kidney and tonsil tissues using NBP1-92396 antibody. Corresponding SLC22A8 RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: SLC22A8 Antibody [NBP1-92396] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

1 Publication
ABCG2/CD338 Antibody (3G8) - BSA Free
NBP2-22124
Western Blot: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of ABCG2 expression in (1) human small intestine, (2) human placenta and (3) mouse placenta tissue extracts.Immunocytochemistry/Immunofluorescence: ABCG2/CD338 Antibody (3G8) [NBP2-22124] - Analysis of Hela cells using ABCG2 mouse mAb (green). DRAQ5 fluorescent DNA dye (blue). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

Mouse Monoclonal
Species Human, Mouse, Primate
Applications WB, ELISA, Flow

8 Publications
FOLR1 Antibody (548908) [Unconjugated]
MAB5646
Western blot shows lysates of human cortex tissue and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (<a class=MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 Monoclonal Antibody (Catalog # MAB5646, filled histogram) or isotype control antibody (<a class=

Mouse Monoclonal
Species Human
Applications WB, Flow, CyTOF-ready

     2 Reviews

12 Publications
RFC1 Antibody [Unconjugated]
AF6457
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RFC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

Sheep Polyclonal
Species Human
Applications WB

Albumin Antibody (188835) [Unconjugated] - Serum
MAB1455
Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, Simple Western, IHC

     3 Reviews

54 Publications
MDR1/ABCB1 Antibody (SN06-42)
NBP2-67667
Western Blot: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Analysis of P Glycoprotein on mouse heart tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody (SN06-42) [NBP2-67667] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MDR1/ABCB1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

3 Publications
MDMX Antibody
NB100-556
Western Blot: MDMX Antibody [NB100-556] - Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HdmX/MDM4 antibody NB100-556 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.Western Blot: MDMX Antibody [NB100-556] - Analysis using the Biotin conjugate of NB100-556. Detection of Jurkat (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.

Rabbit Polyclonal
Species Human
Applications WB, IP

32 Publications
MRP4/ABCC4 Antibody
NB100-1471
Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - Staining Human Prostate tissue. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

9 Publications
MRP1 Antibody (IU2H10) - BSA Free
NB400-156
Dual RNAscope ISH-IHC: MRP1 Antibody (IU2H10) [NB400-156] - Formalin-fixed paraffin-embedded tissue sections of human testis were probed for MRP1 mRNA (ACD RNAScope Probe, catalog # 435591; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using mouse monoclonal (Novus Biologicals catalog # NB400-156) at 7ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to Leydig cells and seminiferous tubule cells.Immunocytochemistry/Immunofluorescence: MRP1 Antibody (IU2H10) [NB400-156] - The MRP-1 antibody was tested in HeLa cells at a 1:50 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

17 Publications
Complement C4 Antibody
NBP3-12295
Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Rabbit Polyclonal
Species Human, Rat, Primate
Applications WB, ELISA, IHC

SLC19A2 Antibody
NBP3-12981
Western Blot: SLC19A2 Antibody [NBP3-12981] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with SLC19A2 antibody (NBP3-12981) diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SLC19A2 Antibody [NBP3-12981] - SLC19A2 antibody detects SLC19A2 protein at cytosol by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC19A2 protein stained by SLC19A2 antibody (NBP3-12981) diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF