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G1 Phase Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the G1 Phase Pathway and Malignant Neoplasms, Neoplasms, Retinoblastoma, Carcinoma, Leukemia. The study of the G1 Phase Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The G1 Phase Pathway has been researched in relation to Cell Cycle, S Phase, Cell Proliferation, Cell Growth, Cell Cycle Arrest. The G1 Phase Pathway complements our catalog of research reagents including antibodies and ELISA kits against CYCLIN E, CYCLIN-DEPENDENT KINASE INHIBITOR P27, CCND1, CDK2, CDK4.

Top Research Reagents

We have 3714 products for the study of the G1 Phase Pathway that can be applied to Chromatin Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NB600-302
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY license.Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). <br/>Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Mouse Monoclonal
Species Human, Mouse, Bovine
Applications WB, Simple Western, ELISA

     4 Reviews

48 Publications
H00027065-M01
Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3 Analysis of D4S234E expression in IMR-32.Western Blot: D4S234E Antibody (1C3) [H00027065-M01] - D4S234E monoclonal antibody (M01), clone 1C3. Analysis of D4S234E expression in PC-12.

Mouse Monoclonal
Species Human, Rat
Applications WB, ELISA, ICC/IF

1 Publication
NBP3-26876
Staining of human colon shows moderate cytoplasmic positivity in glandular cells.Staining of human skeletal muscle shows no positivity in myocytes as expected.

Mouse Monoclonal
Species Human
Applications IHC

NBP1-31308
Immunocytochemistry/Immunofluorescence: CDK4 Antibody [NBP1-31308] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CDK4 protein stained by CDK4 antibody  diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.Immunohistochemistry-Paraffin: CDK4 Antibody [NBP1-31308] - Human gastric cancer, using CDK4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

7 Publications
NBP1-80616
Western Blot: Dynactin 6 Antibody [NBP1-80616] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunohistochemistry-Paraffin: Dynactin 6 Antibody [NBP1-80616] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

2 Publications
NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 40X objective.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     3 Reviews

43 Publications
NBP1-90949
Western Blot: TMED7 Antibody [NBP1-90949] - Analysis in human cell line CAPAN-2.Immunohistochemistry: Rabbit Polyclonal TMED7 Antibody [NBP1-90949] - Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

1 Publication
NBP2-03198
Western Blot: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1.Immunocytochemistry/Immunofluorescence: SSSCA1 Antibody (OTI2F5) [NBP2-03198] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1.

Mouse Monoclonal
Species Human
Applications WB, Flow, ICC/IF

1 Publication
NBP2-24695
Western Blot: Cyclin D1 Antibody [NBP2-24695] - Analysis of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate using Cyclin D1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody  and PicoTect ECL substrate solution were used for this test.Immunohistochemistry-Paraffin: Cyclin D1 Antibody [NBP2-24695] - Formalin-fixed, paraffin-embedded human small intestine stained with Cyclin D1 antibody at 10 ug/ml.

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

4 Publications
NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1) [NBP2-29463] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with p21 Monoclonal Antibody (WA-1).

Mouse Monoclonal
Species Human, Mouse, Chimpanzee
Applications WB, Flow, ICC/IF

24 Publications
AF3994
Western blot shows lysates of MCF-7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <a class=

Rabbit Polyclonal
Species Human
Applications WB

19 Publications
AF4654
Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=Western blot shows lysates of C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

Goat Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

3 Publications
AF6897
Western blot shows lysates of human brain (hypothalamus) tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Human PAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6897) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=PAK3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human PAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6897) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Sheep Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

MAB6495
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 0.1 µg/mL of Human RB1 Monoclonal Antibody (Catalog # MAB6495) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=RB1 was detected in immersion fixed MCF-7 human breast cancer cell line using Human RB1 Monoclonal Antibody (Catalog # MAB6495) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, Simple Western, ICC

5 Publications
NBP2-46085
Western Blot: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCEAL1.Immunohistochemistry: TCEAL1 Antibody (2G10) [NBP2-46085] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(

Mouse monoclonal
Species Human
Applications WB, IHC, IHC-P

NBP2-47560
Immunohistochemistry: 26S proteasome subunit 9 Antibody [NBP2-47560] - Staining of human testis using Anti-PSMD9 antibody NBP2-47560.Immunohistochemistry: 26S proteasome subunit 9 Antibody [NBP2-47560] - Staining of human kidney using Anti-PSMD9 antibody NBP2-47560.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

NB500-106
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Immunocytochemistry/Immunofluorescence: PCNA Antibody (PC10) [NB500-106] - NIH3T3 cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-PCNA Antibody (PC10) NB500-106 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue).  Cells were imaged using a 100X objective and digitally deconvolved.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ChIP

     5 Reviews

48 Publications
NB200-111
Western Blot: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT decreases p53mut stability. T24 cells were transfected with non-targeting control, AKT1, or p14ARF siRNA. Cells were treated with NCS348884 (4 i1/4M), Nutlin3A (5 i1/4M) or DMSO as indicated. Whole cell lysates were probed with the indicated antibodies. Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. <i>Oncotarget</i> (2014))  licensed under a CC-BY license.Immunohistochemistry: p14ARF/CDKN2A Antibody [NB200-111] - Inhibition of AKT modulates p53 stability in-vivo and synergizes with ionizing radiation to inhibit tumor growth( Sections of PSN1 xenografts treated with three consecutive doses of MK-2206 (60 mg/kg). Sections of PSN1 xenografts and in-vitro PSN1 cells fixed and stained with anti-NPM (red) and anti-p14ARF (green). Image collected and cropped by Citeab from the following publication (AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget (2014))  licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     2 Reviews

18 Publications