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Contact Guidance Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Contact Guidance Pathway and Tissue Adhesions, Neoplasms, Cell Invasion, Nervousness, Malignant Neoplasms. The study of the Contact Guidance Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Contact Guidance Pathway has been researched in relation to Cell Migration, Regeneration, Cell Adhesion, Locomotion, Chemotaxis. The Contact Guidance Pathway complements our catalog of research reagents including antibodies and ELISA kits against FN1, PKD2L1, FLT4, NERVE GROWTH FACTOR, VCL.

Top Research Reagents

We have 4269 products for the study of the Contact Guidance Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

PKD2L1 Antibody (4F9)
H00009033-M01
Western Blot: PKD2L1 Antibody (4F9) [H00009033-M01] - PKD2L1 monoclonal antibody (M01), clone 4F9. Analysis of PKD2L1 expression in rat brain.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, PAGE

1 Publication
Vimentin Antibody
NB300-223
Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.Immunohistochemistry: Vimentin Antibody [NB300-223] - Attenuated reactive astrocytosis after stroke in Smad1 cKO mice. Enlarged IHC images of boxed areas at the cortical peri-infarct area with the indicated reactive astrocyte markers GFAP, Nestin, and Vimentin. Enlarged images of GFAP IHC highlight the hypertrophic morphology of GFAP+ astrocytes in mutants. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0136967), licensed under a CC-BY license.

Chicken Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     6 Reviews

70 Publications
GRP78/HSPA5 Antibody - BSA Free
NBP1-06274
Simple Western: GRP78/HSPA5 Antibody [NBP1-06274] - Image shows a specific band for GRP78 in 0.1 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.Western Blot: GRP78/HSPA5 Antibody [NBP1-06274] - C. elegans granulins impair organismal fitness and resistance to ER stress. Total worm lysates from synchronized day 1 adult granulin-expressing animals were immunoblotted with an anti-GRP78/HSPA5 antibody (3 biological replicates). Anti-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pgen.1008295), licensed under a CC-BY license.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, Flow

     2 Reviews

43 Publications
RhoD Antibody
NBP1-58359
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/mlImmunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

Afamin Antibody
NBP1-89156
Immunohistochemistry-Paraffin: Afamin Antibody [NBP1-89156] - Staining of human kidney.Immunohistochemistry: Afamin Antibody [NBP1-89156] - Staining of human kidney shows strong cytoplasmic and extracellular material positivity in cells in tubules.

Rabbit Polyclonal
Species Human
Applications IHC, IHC-P

ATP Citrate Lyase Antibody
NBP1-90267
Western Blot: ATP Citrate Lyase Antibody [NBP1-90267] - Analysis using Anti-ACLY antibody NBP1-90267 (A) shows similar pattern to independent antibody NBP1-90268 (B).Immunocytochemistry/Immunofluorescence: ATP Citrate Lyase Antibody [NBP1-90267] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

Fibronectin Antibody - BSA Free
NBP1-91258
Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (https://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY license.Immunocytochemistry/Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NBP1-91258 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     11 Reviews

56 Publications
RACK1/GNB2L1 Antibody (OTI2D8)
NBP2-00612
Western Blot: RACK1/GNB2L1 Antibody (2D8) [NBP2-00612] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GNB2L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GNB2L1.Immunohistochemistry-Paraffin: RACK1/GNB2L1 Antibody (2D8) [NBP2-00612] - Staining of paraffin-embedded Human lymph node tissue using anti-GNB2L1 mouse monoclonal antibody.

Mouse Monoclonal
Species Human
Applications WB, Flow, IHC

SS18L1 Antibody
NBP2-20486
Western Blot: SS18L1 Antibody [NBP2-20486] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/Immunofluorescence: SS18L1 Antibody [NBP2-20486] - Immunofluorescence analysis of paraformaldehyde-fixed A431, using antibody at 1:500 dilution.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ICC/IF, IHC

VEGFR3/Flt-4 Antibody [Unconjugated]
AF743
bEnd.3 cells, a mouse endothelioma cell line, was stained with Goat Anti-Mouse VEGFR3/Flt‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF743, filled histogram) or isotype control antibody (Catalog # <a class=Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha -GFP, green) near kdr-l positive ( alpha -RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries and veins. NG2 (red) labels pericytes and smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, and Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), and VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single and double-labelled cells in 2-month old mouse meninges. VEGFR3 and LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 and LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Mouse
Applications WB, Flow, CyTOF-ready

     3 Reviews

137 Publications
CD44 Antibody (2C5) [Unconjugated] - s Pan Specific
BBA10
Western blot shows lysates of PC-3 human prostate cancer cell line and human tonsil tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=CD44 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Mouse Anti-Human CD44 s Pan Specific Monoclonal Antibody (Catalog # BBA10) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications WB, Simple Western, Flow

     5 Reviews

49 Publications
Vinculin Antibody (728526) [Unconjugated]
MAB6896
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Vinculin was detected in immersion fixed paraffin-embedded sections of human uterus using Mouse Anti-Human Vinculin Monoclonal Antibody (Catalog # MAB6896) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, IHC

     3 Reviews

19 Publications
LIGHT/TNFSF14 [Unconjugated]
664-LI
<P align=left>Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED<SUB>50</SUB> is 1-4 ng/mL.</P><p align=


Species Human
Applications BA

21 Publications
beta-NGF [Unconjugated]
256-GF
Recombinant Human beta -NGF (Catalog # 256‑GF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED<SUB>50</SUB> for this effect is 0.2‑2 ng/mL. 1 µg/lane of Recombinant Human beta -NGF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.


Species Human
Applications BA

57 Publications
PMEL17/SILV Antibody (HMB45)
NBP2-44520
Western Blot: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV antibody (HMB45).Immunohistochemistry: PMEL17/SILV Antibody (HMB45) [NBP2-44520] - PMEL17 (red) was detected in human skin (melanoma) using PMEL17-PE antibody (1:200) in PBS for 1 hour. Nuclei were stained with DAPI (blue). Image from a verified customer review. Image using the PE format of this antibody.

Mouse Monoclonal
Species Human, Canine (Negative), Rat (Negative)
Applications WB, Flow, ICC/IF

8 Publications
GFAP Antibody
NB300-141
Immunohistochemistry: GFAP Antibody [NB300-141] - Analysis of a rat cerebellum section stained with rabbit polyclonal antibody to GFAP, NB300-141, dilution 1:5000 in green and mouse monoclonal antibody to MeCP2, dilution 1:500, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 1 hour, cut to 45 uM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic cells and the processes of Bergmann glia in the molecular layer. The MeCP2 antibody specifically labels nuclei of certain neurons.Immunocytochemistry/Immunofluorescence: GFAP Antibody [NB300-141] - Rat neurons stained with Neurofilament Heavy antibody NB300-217 (red) and GFAP antibody NB300-141 (green).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

     12 Reviews

108 Publications
Rhodopsin Antibody (4D2)
NBP2-59690
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS inner segment; ONL outer nuclear layer; OPL outer plexiform layer; INL inner nuclear layer; IPL inner plexiform layer; GCL ganglion cell layer..

Mouse Monoclonal
Species Amphibian, Avian, Fish
Applications WB, ELISA, ICC/IF

     1 Review

6 Publications
Tenascin C Antibody (4C8MS) - BSA Free
NB110-68136
Flow (Intracellular): Tenascin C Antibody (4C8MS) [NB110-68136] - Figure A: Intracellular stain performed on U87MG Cells with Tenascin C (4C8MS) antibody NB110-68136 (blue) and a matched isotype control NBP1-97005 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Figure B: U87MG Cells were either untreated (orange) or treated with 3uM Monensin (blue). An intracellular stain was performed with Tenascin C (4C8MS) antibody NB110-68136. Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody [F0101B, R&D Systems].Western Blot: Tenascin C Antibody (4C8MS) [NB110-68136] - Regulation of Tenascin C expression and its effect on fibrotic responses. Confluent foreskin fibroblasts were incubated with TGF-beta (10 ng ml-1 or indicated concentrations) or Tenascin C (TNC) for 24 or 72 h or indicated periods. Whole-cell lysates, culture media and RNA were examined by western analysis (upper panels) and qPCR (lower panel). Representative immunoblots or qPCR results (means+/-s.e.m. of triplicate determinations). S, secreted; L, lysates. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/doifinder/10.1038/ncomms11703), licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

     2 Reviews

26 Publications
NuMA Antibody
NB500-174
Western Blot: NuMA Antibody [NB500-174] - Analysis of NuMA expression in Cos7 whole cell lysate.Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - Human HeLa cells fixed with 3.5% formaldehyde and stained with NB500-174 at 1:1000. The staining pattern shows the typical pattern for NuMA in the cell nucleus during interphase and on spindles during mitosis.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ICC/IF

27 Publications