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![Western Blot: NuMA Antibody [NB500-174] - Analysis of NuMA expression in Cos7 whole cell lysate.](http://images.novusbio.com/fullsize/NuMA-Antibody-Western-Blot-NB500-174-img0001.jpg "Western Blot: NuMA Antibody [NB500-174] - Analysis of NuMA expression in Cos7 whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - Human HeLa cells fixed with 3.5% formaldehyde and stained with NB500-174 at 1:1000. The staining pattern shows the typical pattern for NuMA in the cell nucleus during interphase and on spindles during mitosis.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-174-img0003.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human placenta using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Strong nuclear with moderate cytoplasmic immuno-reactivity of NuMA was observed in syncytiotrophoblast, cytiotrophoblasts, Hofbauer cells and the endothelial cells of chorionic villi capillaries. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-174-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - NuMA antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-174-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NuMA Antibody [NB500-174] - Dynactin and NuMA display specific, steady-state binding at mitotic minus-ends. See also Figure 1-figure supplement 1 and Videos 1-3. Representative immunofluorescence image showing co-localization of NuMA (green) and p150 (dynactin subunit; cyan) at microtubule minus-ends in mitotic PtK2 cells (post-NEB) fixed after washout of 5 uM nocodazole. Scale bar, 10 um. Inset: zoom of white box, with 1 um scale bar. Image collected and cropped by CiteAb from the following publication (//elifesciences.org/articles/29328), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-174-img0024.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal breast using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The breast ductal/acinar epithelial cells, the myoepithelial cells and some cells in the surrounding connective tissue depicted a strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. The intra-lobular and the surrounding connective tissue also developed weak/potentially non-specific staining. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0007.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal spleen using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Most of the cells developed a strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0008.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal skin using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. Almost all the cells in the epidermal layer and the cells in the connective tissue of dermal layer depicted very strong nuclear along with moderate cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0009.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal brain using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells in the brain tissue depicted strong specific nuclear along with weak cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0010.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal kidney using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The cells of various tubules/ducts and Bowman's capsule in the renal tissue depicted strong nuclear along with relatively weak cytoplasmic immuno-positivity for NuMA protein. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0011.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NB500-174] - Analysis of FFPE tissue section of human normal prostate using rabbit polyclonal NuMA antibody (NB500-174) at 5 ug/mL. The prostatic alveolar glandular epithelium as well as the surrounding fibromuscular stroma cells showed a strong nuclear immuno-reactivity for NuMA protein. Almost all the cells developed some cytoplasmic staining also. 10X Magnification.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NB500-174-img0012.jpg)
![Simple Western: NuMA Antibody [NB500-174] - Image shows a specific band for NuMA in 0.2 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.](http://images.novusbio.com/fullsize/NuMA-Antibody-Simple-Western-NB500-174-img0023.jpg)
![Western Blot: NuMA Antibody [NBP2-54672] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/NuMA-Antibody-Western-Blot-NBP2-54672-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: NuMA Antibody [NBP2-54672] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human fallopian tube, gastrointestinal, kidney and lymphoid tissues using Anti-NUMA1 antibody NBP2-54672 (A) shows similar protein distribution across tissues to independent antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202395858..jpg)
![Immunocytochemistry/Immunofluorescence: NuMA Antibody [NBP2-54672] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54672-img0003.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202310024..jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202310540..jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human tonsil shows strong nuclear positivity in non-germinal center cells and germinal center cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-10820231078..jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54672] -Staining of human kidney shows strong nuclear positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/antibody/nbp2-54672_rabbit-polyclonal-numa-antibody-immunohistochemistry-paraffin-108202310820..jpg)
![Western Blot: NuMA Antibody [NB100-74636] - Detection of Human NUMA on HeLa whole cell lysate using NB100-74636. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody NB100-74637.](http://images.novusbio.com/fullsize/NuMA-Antibody-Western-Blot-NB100-74636-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NuMA Antibody [NB100-74636] - Detection of Human NUMA on HeLa whole cell lysate using NB100-74636. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody NB100-74637.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NuMA Antibody [NB100-74636] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NUMA used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-NB100-74636-img0010.jpg)
![Western Blot: NuMA Antibody (6D4I4) [NBP3-16411] - Western blot analysis of extracts of various cell lines, using NuMA1 Rabbit mAb (NBP3-16411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/NuMA-Antibody-6D4I4-Western-Blot-NBP3-16411-img0004.jpg "Western Blot: NuMA Antibody (6D4I4) [NBP3-16411] - Western blot analysis of extracts of various cell lines, using NuMA1 Rabbit mAb (NBP3-16411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry of paraffin-embedded mouse brain using NuMA1 Rabbit mAb (NBP3-16411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NuMA-Antibody-6D4I4-Immunohistochemistry-Paraffin-NBP3-16411-img0003.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry of paraffin-embedded rat brain using NuMA1 Rabbit mAb (NBP3-16411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NuMA-Antibody-6D4I4-Immunohistochemistry-Paraffin-NBP3-16411-img0001.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody (6D4I4) [NBP3-16411] - Immunohistochemistry of paraffin-embedded human colon using NuMA1 Rabbit mAb (NBP3-16411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NuMA-Antibody-6D4I4-Immunohistochemistry-Paraffin-NBP3-16411-img0002.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-NUMA1 antibody NBP2-54673 (A) shows similar protein distribution across tissues to independent antibody NBP2-54672 (B).](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0003.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-NUMA1 antibody NBP2-54673 (A) shows similar protein distribution across tissues to independent antibody NBP2-54672 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0006.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human kidney.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0004.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human colon.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0005.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody [NBP2-54673] - Staining of human pancreas.](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-Paraffin-NBP2-54673-img0007.jpg)
![Western Blot: NuMA Antibody (1C5) [H00004926-M01] - NUMA1 monoclonal antibody (M01), clone 1C5 Analysis of NUMA1 expression in K-562.](http://images.novusbio.com/fullsize/NuMA-Antibody-1C5-Western-Blot-H00004926-M01-img0006.jpg "Western Blot: NuMA Antibody (1C5) [H00004926-M01] - NUMA1 monoclonal antibody (M01), clone 1C5 Analysis of NUMA1 expression in K-562.")
![Immunocytochemistry/Immunofluorescence: NuMA Antibody (1C5) [H00004926-M01] - Immunofluorescence of monoclonal antibody to NUMA1 on HeLa cell . [antibody concentration 10 ug/ml]](http://images.novusbio.com/fullsize/NuMA-Antibody-1C5-Immunocytochemistry-Immunofluorescence-H00004926-M01-img0002.jpg)
![Immunoprecipitation: NuMA Antibody (1C5) [H00004926-M01] - Analysis of NUMA1 transfected lysate using anti-NUMA1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NUMA1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/NuMA-Antibody-1C5-Immunoprecipitation-H00004926-M01-img0004.jpg)
![ELISA: NuMA Antibody (1C5) [H00004926-M01] - Detection limit for recombinant GST tagged NUMA1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NuMA-Antibody-1C5-ELISA-H00004926-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: NuMA Antibody (SPM300) [NBP2-45227] - Human Tonsil stained with NuMA Monoclonal Antibody (SPM300)](http://images.novusbio.com/fullsize/NuMA-Antibody-SPM300-Immunohistochemistry-Paraffin-NBP2-45227-img0001.jpg "Immunohistochemistry-Paraffin: NuMA Antibody (SPM300) [NBP2-45227] - Human Tonsil stained with NuMA Monoclonal Antibody (SPM300)")
![ELISA: Human NuMA ELISA Kit (Colorimetric) [NBP3-39200] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39200_human-numa-elisa-kit-colorimetric-2110202415355097.png "ELISA: Human NuMA ELISA Kit (Colorimetric) [NBP3-39200] - Standard Curve Reference")
![ELISA: Human NuMA - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39201] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39201_human-numa-ready-to-use-elisa-kit-colorimetric-2110202415295680.png "ELISA: Human NuMA - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39201] - Standard Curve Reference")