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Basophil Activation Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Basophil Activation Pathway and Allergy, Anaphylaxis, Immediate Hypersensitivity, Food Allergy, Urticaria. The study of the Basophil Activation Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Basophil Activation Pathway has been researched in relation to Hypersensitivity, Anaphylaxis, Sensitization, Cell Activation, Pathogenesis. The Basophil Activation Pathway complements our catalog of research reagents including antibodies and ELISA kits against FCEPSILONRI, TRYPTASE, AGXT, BAAT, C4A.

Top Research Reagents

We have 4399 products for the study of the Basophil Activation Pathway that can be applied to Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NOD2 Antibody (2D9) - BSA Free
NB100-524
Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY license.Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF

     1 Review

26 Publications
ENPP-3/CD203c Antibody (NP4D6) - BSA Free
NBP1-44643
Immunocytochemistry/Immunofluorescence: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of activated human basophil by FITC-conjugated anti-CD63 (clone MEM-259, green) and PE-conjugated anti-CD203c (clone NP4D6, red); merged signal yellow. DNA visualized by DAPI (blue).Flow Cytometry: ENPP-3/CD203c Antibody (NP4D6) [NBP1-44643] - Staining of human peripheral blood by anti-CD203c antibody (NP4D6) FITC.

Mouse Monoclonal
Species Human
Applications Flow, ICC/IF, CyTOF-ready

AGXT Antibody
NBP1-89200
Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Analysis in human liver and pancreas tissues. Corresponding AGXT RNA-seq data are presented for the same tissues.Immunohistochemistry-Paraffin: AGXT Antibody [NBP1-89200] - Staining of human endometrium, fallopian tube, skin and testis using Anti-NEK1 antibody (A) NBP1-89200 shows similar protein distribution across tissues to independent antibody NBP2-34198 (B).

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, IHC

3 Publications
WASP Antibody
NB300-996
Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunohistochemistry-Paraffin: WASP Antibody [NB300-996] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, Flow, ICC/IF

1 Publication
Calpastatin Antibody (OTI1E7)
NBP2-01819
Western Blot: Calpastatin Antibody (1E7) [NBP2-01819] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Calpastatin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Calpastatin.Immunohistochemistry-Paraffin: Calpastatin Antibody (1E7) [NBP2-01819] Staining of paraffin-embedded Human prostate tissue using anti-Calpastatin mouse monoclonal antibody.

Mouse Monoclonal
Species Human
Applications WB, Flow, IHC

Vitrin Overexpression Lysate
NBP2-11460
Western Blot: Vitrin Overexpression Lysate (Adult Normal) [NBP2-11460] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Vitrin.


Species Human
Applications WB

PAF49 Antibody
NBP2-19676
Western Blot: PAF49 Antibody [NBP2-19676] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAF49 antibody diluted at 1:1000.Immunocytochemistry/Immunofluorescence: PAF49 Antibody [NBP2-19676] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: PAF49 protein stained by PAF49 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:500. Blue: Hoechst 33342 staining.

Rabbit Polyclonal
Species Human
Applications WB, ICC/IF, IHC

DNER Antibody [Unconjugated]
AF2254
DNER was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2254) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=DNER does not activate, function with, or bind to Notch, but known Notch ligand DLL1 does.(A) Pooled luciferase results from 4 separate experiments (normalized to the mean of empty vector in each experiment). U2OS cells were transfected with ligand (DLL1, DNER, or EV), and separately a population of U2OS cells was transfected to express Notch, the control luciferase Renilla, and TP1, a promoter that expresses firefly luciferase when Notch is activated. The two populations were co-cultured 24 hours after transfection (trans configuration), and activity read after an additional 24–48 hours of incubation. (B) C2C12 cells (myoblasts) were incubated with differentiation media (2% horse serum) that either had pre-clustered DLL1-fc (1:1), pre-clustered DNER-fc (1:1), un-clustered DNER-fc, or fc only, all at a ratio of 1:150 in media. Cells were incubated for 72 hours, then fixed, and stained for the presence of myosin heavy chain (MHC) and nuclei. By measuring the percent of total nuclei that were inside of differentiated MHC positive myotubes, fusion indexes were calculated. (C) DNER (top left, green) transfected U2OS cells were not labeled by pre-clustered Notch-fc (top middle, red) but DLL1 (bottom left, green) transfected U2OS cells were labeled by pre-clustered Notch-fc (bottom middle, red). Merged images are shown at far right. Scale 10 μM. **** = p value <0.0001. *** = p value 0.002. ns = not significant. DLL1 = Delta-like 1, a known Notch Ligand, DNER = Delta/Notch-like epidermal growth factor (EGF) related receptor, GSI = gamma -secretase inhibitor, fc only = rabbit anti-human-fc. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0161157), licensed under a CC-BY license. Not internally tested by R&D Systems.

Goat Polyclonal
Species Mouse
Applications WB, IHC

12 Publications
CTLA-4 [Unconjugated]
7268-CT
Recombinant Human CTLA-4 Fc Chimera (Catalog # 7268-CT) inhibits IL-2 secretion by stimulated Jurkat human acute Tcell leukemia cells. The ED<sub>50</sub> for this effect is 0.03-0.15 μg/mL whenstimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # <a class=


Species Human
Applications BA

3 Publications
IL-4 [Unconjugated]
6507-IL/CF
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED<sub>50</sub> for this effect is 0.05-0.2 ng/mL.


Species Human
Applications BA

3 Publications
IL-3 [Unconjugated]
203-IL
Recombinant Human IL-3 Protein (Catalog # 203-IL) has a molecular weight (MW) of 14.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer. Recombinant Human IL-3 (Catalog # 203-IL) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED<sub>50</sub> for this effect is 0.02-0.1 ng/mL.


Species Human
Applications BA

216 Publications
IL-13 [Biotin]
DY413
N/A IL-13 [Biotin]


Species Mouse
Applications ELISA

148 Publications
CD63 Antibody (H5C6) - BSA Free
NBP2-42225
Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.

Mouse Monoclonal
Species Human, Canine
Applications WB, DB, EM

     5 Reviews

57 Publications
HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free
NBP2-79843
Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of PFA-fixed Ramos cells. HLA DQ/DR/DP Recombinant Rabbit Monoclonal Antibody (HLA DQ/DR/DP/2967R) followed by goat anti-rabbit IgG-CF488 (green). Nuclei stained with RedDot.

Rabbit Monoclonal
Species Human
Applications WB, ELISA, Flow

Complement Component C5aR1 Antibody (347234) [Unconjugated]
MAB36481
HEK293 human embryonic kidney cell line transfected with either (A) human C5aR or (B) irrelevant protein and eGFP was stained with Mouse Anti-Human C5aR Monoclonal Antibody (Catalog # MAB36481) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=Complement Component C5aR1 was detected in immersion fixed U937 human histiocytic lymphoma cell line (positive) and SH-SY5Y human neuroblastoma cell line (negative control) using Mouse Anti-Human Complement Component C5aR1 Monoclonal Antibody (Catalog # MAB36481) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

Mouse Monoclonal
Species Human
Applications Flow, IHC, CyTOF-ready

BAAT Antibody - BSA Free
NBP2-92503
Western Blot: BAAT Antibody [NBP2-92503] - Analysis of extracts of various cell lines, using BAAT at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.Immunocytochemistry/Immunofluorescence: BAAT Antibody [NBP2-92503] - Analysis of Hep G2 cells using BAAT Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ELISA, ICC/IF

IgE Antibody (1E7)
NBP3-11412

Mouse Monoclonal
Species Sheep
Applications WB, ELISA, IHC

5 Publications
Complement C4 Antibody
NBP3-12295
Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.Immunohistochemistry-Paraffin: Complement C4 Antibody [NBP3-12295] - Baboon liver. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

Rabbit Polyclonal
Species Human, Rat, Primate
Applications WB, ELISA, IHC