| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52587-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3070-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3070-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB30701-SP | |||
![Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/WASP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-996-img0007.jpg "Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: WASP Antibody [NB300-996] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/WASP-Antibody-Immunohistochemistry-Paraffin-NB300-996-img0004.jpg)
![Flow Cytometry: WASP Antibody [NB300-996] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/WASP-Antibody-Flow-Cytometry-NB300-996-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/WASP-Antibody-Immunocytochemistry-Immunofluorescence-NB300-996-img0005.jpg)
![Western Blot: WASP Antibody (7B10E4) [NBP2-52587] - Analysis using WAS mAb against human WAS (AA: 57-170) recombinant protein. (Expected MW is 39 kDa)](http://images.novusbio.com/fullsize/WASP-Antibody-7B10E4-Western-Blot-NBP2-52587-img0004.jpg "Western Blot: WASP Antibody (7B10E4) [NBP2-52587] - Analysis using WAS mAb against human WAS (AA: 57-170) recombinant protein. (Expected MW is 39 kDa)")
![Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of paraffin-embedded colon cancer tissues using WAS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/WASP-Antibody-7B10E4-Immunohistochemistry-Paraffin-NBP2-52587-img0003.jpg)
![Flow Cytometry: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of Hela cells using WAS mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/WASP-Antibody-7B10E4-Flow-Cytometry-NBP2-52587-img0001.jpg)
![Western Blot: WASP Antibody (7B10E4) [NBP2-52587] - Analysis using WAS mAb against HEK293 (1) and WAS (AA: 57-170)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/WASP-Antibody-7B10E4-Western-Blot-NBP2-52587-img0005.jpg)
![Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of paraffin-embedded ovarian cancer tissues using WAS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/WASP-Antibody-7B10E4-Immunohistochemistry-Paraffin-NBP2-52587-img0002.jpg)
![ELISA: WASP Antibody (7B10E4) [NBP2-52587] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/WASP-Antibody-7B10E4-ELISA-NBP2-52587-img0006.jpg)
![Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Detection of Human Phospho WASP (S483/S484) by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho WASP (S483/S484) antibody NB100-2307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/WASP-[p-Ser483--p-Ser484]-Antibody-Western-Blot-NB100-2307-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: WASP [p Ser483, p Ser484] Antibody [NB100-2307] - Detection of Human Phospho WASP (S483/S484) by Western Blot. Samples: Whole cell lysate (50 ug) from Jurkat and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Phospho WASP (S483/S484) antibody NB100-2307 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: WASP Antibody [NBP1-87827] - Analysis in human lymph node and kidney tissues using NBP1-87827 antibody. Corresponding WASP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-87827-img0006.jpg "Immunohistochemistry-Paraffin: WASP Antibody [NBP1-87827] - Analysis in human lymph node and kidney tissues using NBP1-87827 antibody. Corresponding WASP RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: WASP Antibody [NBP1-87827] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp and cells in white pulp.](http://images.novusbio.com/fullsize/WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-87827-img0002.jpg)
![Immunohistochemistry-Paraffin: WASP Antibody [NBP1-87827] - Staining of human appendix shows high expression.](http://images.novusbio.com/fullsize/WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-87827-img0003.jpg)
![Immunohistochemistry-Paraffin: WASP Antibody [NBP1-87827] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/WASP-Antibody-Immunohistochemistry-Paraffin-NBP1-87827-img0004.jpg)
![Western Blot: WASP Antibody (SR2069) [NBP3-22175] - Western blot analysis using NBP3-22175 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/nbp3-22175_rabbit-wasp-mab-sr2069-5720231716188.jpg "Western Blot: WASP Antibody (SR2069) [NBP3-22175] - Western blot analysis using NBP3-22175 in Jurkat cell lysate.")
![Western Blot: WASP Antibody (JE36-82) [NBP3-33098] - Western blot analysis of WASP on different lysates with Rabbit anti-WASP antibody (NBP3-33098) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33098) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33098_rabbit-wasp-mab-je36-82-288202414191516.jpg "Western Blot: WASP Antibody (JE36-82) [NBP3-33098] - Western blot analysis of WASP on different lysates with Rabbit anti-WASP antibody (NBP3-33098) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: THP-1 cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33098) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: WASP Antibody (JE36-82) [NBP3-33098] - Immunocytochemistry analysis of Jurkat cells labeling WASP with Rabbit anti-WASP antibody (NBP3-33098) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-WASP antibody (NBP3-33098) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33098_rabbit-wasp-mab-je36-82-2920241192340.jpg)
![Flow Cytometry: WASP Antibody (JE36-82) [NBP3-33098] - Flow cytometric analysis of Jurkat cells labeling WASP. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33098, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33098_rabbit-wasp-mab-je36-82-29202416453296.jpg)
![SDS-Page: Recombinant Human WASP Protein [H00007454-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00007454-Q01-1.jpg "SDS-Page: Recombinant Human WASP Protein [H00007454-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: WASp Overexpression Lysate (Adult Normal) [NBP2-11140] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WASp.](http://images.novusbio.com/fullsize/WASp-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11140-img0001.jpg "Western Blot: WASp Overexpression Lysate (Adult Normal) [NBP2-11140] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WASp.")
![Western Blot: WASP Antibody [NBP2-99085] - Anti-WASP rabbit polyclonal antibody at 1:500 dilution. Lane A: U937 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 57 kDa](http://images.novusbio.com/fullsize/WASP-Antibody-Western-Blot-NBP2-99085-img0001.jpg "Western Blot: WASP Antibody [NBP2-99085] - Anti-WASP rabbit polyclonal antibody at 1:500 dilution. Lane A: U937 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 53 kDa. Observed band size: 57 kDa")