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![Western Blot: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Western blot analysis of extracts of various cell lines, using HLA DQA1 Rabbit mAb (NBP3-15364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-7W0M7-Western-Blot-NBP3-15364-img0004.jpg "Western Blot: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Western blot analysis of extracts of various cell lines, using HLA DQA1 Rabbit mAb (NBP3-15364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Immunohistochemistry of paraffin-embedded mouse spleen using HLA DQA1 Rabbit mAb (NBP3-15364) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-7W0M7-Immunohistochemistry-Paraffin-NBP3-15364-img0003.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Immunohistochemistry of paraffin-embedded rat spleen using HLA DQA1 Rabbit mAb (NBP3-15364) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-7W0M7-Immunohistochemistry-Paraffin-NBP3-15364-img0001.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody (7W0M7) [NBP3-15364] - Immunohistochemistry of paraffin-embedded human spleen using HLA DQA1 Rabbit mAb (NBP3-15364) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-7W0M7-Immunohistochemistry-Paraffin-NBP3-15364-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining in human lung and kidney tissues using anti-HLA-DQA1 antibody. Corresponding HLA-DQA1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining in human lung and kidney tissues using anti-HLA-DQA1 antibody. Corresponding HLA-DQA1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HLA DQA1 Antibody [NBP1-84550] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human cell line Daudi.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Western-Blot-NBP1-84550-img0004.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0006.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP1-84550-img0005.jpg)
![Western Blot: HLA DQA1 Antibody [NBP3-03602] - Analysis of extracts of various cell lines, using HLA DQA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Western-Blot-NBP3-03602-img0003.jpg "Western Blot: HLA DQA1 Antibody [NBP3-03602] - Analysis of extracts of various cell lines, using HLA DQA1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP3-03602] - Paraffin-embedded human esophagus using HLA DQA1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP3-03602-img0002.jpg)
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP3-03602] - Paraffin-embedded human prostate using HLA DQA1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP3-03602-img0001.jpg)
![Western Blot: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DQA1 antibody [HL2139] (NBP3-25507) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25507_rabbit-hla-dqa1-mab-hl2139-azide-and-bsa-free-212024938317.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA-DQA1 antibody [HL2139] (NBP3-25507) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HLA DQA1 Antibody (HL2139) - Azide and BSA Free [NBP3-25507] - HLA-DQA1 antibody [HL2139] detects HLA-DQA1 protein at cell membrane by immunofluorescent analysis. Sample: Raji cells were fixed in ice-cold MeOH for 5 min. Green: HLA-DQA1 stained by HLA-DQA1 antibody [HL2139] (NBP3-25507) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25507_rabbit-hla-dqa1-mab-hl2139-azide-and-bsa-free-212024941447.jpg)
![Western Blot: HLA DQA1 Antibody [NBP3-13483] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HLA DQA1 antibody (NBP3-13483) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Western-Blot-NBP3-13483-img0001.jpg "Western Blot: HLA DQA1 Antibody [NBP3-13483] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HLA DQA1 antibody (NBP3-13483) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/ Immunofluorescence: HLA DQA1 Antibody [NBP3-13483] - HLA DQA1 antibody detects HLA DQA1 protein at cell membrane by immunofluorescent analysis.Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HLA DQA1 stained by HLA DQA1 antibody (NBP3-13483) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13483_rabbit-hla-dqa1-pab-91020242063377.jpg)
![Proximity Ligation Assay: HLA DQA1 Antibody (1A3) [H00003117-M01] - Analysis of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with anti-CD74 rabbit purified polyclonal 1:1200 and anti-HLA-DQA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-1A3-Proximity-Ligation-Assay-H00003117-M01-img0001.jpg "Proximity Ligation Assay: HLA DQA1 Antibody (1A3) [H00003117-M01] - Analysis of protein-protein interactions between CD74 and HLA-DQA1. HeLa cells were stained with anti-CD74 rabbit purified polyclonal 1:1200 and anti-HLA-DQA1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![ELISA: HLA DQA1 Antibody (1A3) [H00003117-M01] - Detection limit for recombinant GST tagged HLA-DQA1 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-1A3-ELISA-H00003117-M01-img0003.jpg)
![Western Blot: HLA DQA1 Overexpression Lysate (Adult Normal) [NBL1-11584] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HLA DQA1.](http://images.novusbio.com/fullsize/HLA-DQA1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11584-img0002.jpg "Western Blot: HLA DQA1 Overexpression Lysate (Adult Normal) [NBL1-11584] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HLA DQA1.")
![Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP2-97828] - Immunochemical staining of human HLA DQA1 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/HLA-DQA1-Antibody-Immunohistochemistry-Paraffin-NBP2-97828-img0001.jpg "Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP2-97828] - Immunochemical staining of human HLA DQA1 in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HLA-DQ-Antibody-SPV-L3-Flow-Cytometry-NBP2-45041-img0001.jpg "Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).")