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Apical Constriction Pathway Bioinformatics

Disease and disorder research has been conducted in relation to the Apical Constriction Pathway and Stenosis, Tissue Adhesions, Complete Obstruction, Pain, Dental Pulp Necrosis. The study of the Apical Constriction Pathway has been mentioned in research publications which can be found using our bioinformatics tool below. The Apical Constriction Pathway has been researched in relation to Gastrulation, Localization, Neural Tube Closure, Tube Closure, Tube Formation. The Apical Constriction Pathway complements our catalog of research reagents including antibodies and ELISA kits against RHO, RHOD, APEX1, PARD3, MYH14.

Top Research Reagents

We have 2520 products for the study of the Apical Constriction Pathway that can be applied to Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot from our catalog of antibodies and ELISA kits.

NBP1-58359
Western Blot: RhoD Antibody [NBP1-58359] - Transfected 293T, Antibody Titration: 0.2-1 ug/mlImmunohistochemistry: RhoD Antibody [NBP1-58359] - Human Liver Tissue Observed Staining: Cytoplasm in sinusoids of liver Primary Antibody Concentration: 1 : 100 Other Working Concentrations: 1/600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Expos

Rabbit Polyclonal
Species Human
Applications WB, IHC, IHC-P

NBP1-88861
Western Blot: PARD3/Par3 Antibody [NBP1-88861] - Analysis in human cell lines A-549 and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.Simple Western: PARD3/Par3 Antibody [NBP1-88861] - Simple Western lane view shows a specific band for PARD3/Par3 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, Simple Western, ICC/IF

     2 Reviews

10 Publications
NB300-996
Immunocytochemistry/Immunofluorescence: WASP Antibody [NB300-996] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).Immunohistochemistry-Paraffin: WASP Antibody [NB300-996] - Staining of paraffin embedded Human Spleen. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Goat Polyclonal
Species Human
Applications WB, Flow, ICC/IF

1 Publication
NBP2-00555
Western Blot: VASP Antibody (OTI4D6) [NBP2-00555] - Analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.Immunocytochemistry/Immunofluorescence: VASP Antibody (OTI4D6) [NBP2-00555] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF

1 Publication
NBP2-14871
Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 stained by Calmodulin 2 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338]  diluted at 1:500. Blue: Fluoroshield with DAPI.

Rabbit Polyclonal
Species Mouse, Rat
Applications WB, ICC/IF

1 Publication
NBP2-15669
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Upregulation of miRNA inhibits the expression level of target genes in HK-2 cells and CKD patients. Western Blot shows that hsa-miR-4709-3p inhibit the protein level of ITGB8 and CALM3 respectively. NC: scrambled negative control transfection;4709: hsa-miR-4709-3p transfection. Image collected and cropped by CiteAb from the following publication (https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-019-1512-x) licensed under a CC-BY license.Western Blot: Calmodulin 3 Antibody [NBP2-15669]- Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin antibody  diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody  was used to detect the primary antibody.

Rabbit Polyclonal
Species Mouse, Rat, Zebrafish
Applications WB, IHC, IHC-WhMt

1 Publication
NB120-2860
Western Blot: Calmodulin Antibody (2D1) [NB120-2860] - Purified Calmodulin using NB120-2860 and NB120-5494.Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Staining of rat brain.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ELISA, Flow

3 Publications
MAB4470
Myosin Heavy Chain was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human Myosin Heavy Chain Monoclonal Antibody (Catalog # MAB4470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=    Myosin  Heavy Chain was detected in immersion fixed paraffin-embedded sections of  human skeletal muscle using Mouse Anti-Myosin Heavy Chain Monoclonal Antibody  (Catalog # MAB4470) at 5 µg/mL for 1 hour at room  temperature followed by incubation with the Anti-Mouse IgG  VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

Mouse Monoclonal
Species Multi-Species
Applications WB, IHC, ICC

     1 Review

159 Publications
AF231
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231, filled histogram) or isotype control antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB, Simple Western, Flow

30 Publications
NBP2-37364
Western Blot: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis using TWIST1 mouse mAb against NIH/3T3 (1), JURKAT (2), HELA (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.Immunocytochemistry/Immunofluorescence: Twist-1 Antibody (10E4E6) [NBP2-37364] - Analysis of HeLa cells using TWIST1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ELISA, Flow

     1 Review

9 Publications
NBP2-43546
Western Blot: p114RhoGEF Antibody (1972) [NBP2-43546] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ARHGEF18 antibody [1972] dilution: 1:1000.Immunohistochemistry-Paraffin: p114RhoGEF Antibody (1972) [NBP2-43546] - Mouse thymus gland. ARHGEF18 stained by ARHGEF18 antibody [1972] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

1 Publication
NBP2-59690
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Lane 1: MW ladder. Lane 2: Human A549 Cells 15 ug). Load: 15 ug. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000 for 2.5 hours at RT with shaking . Secondary Antibody: Goat anti-mouse IgG:HRP at 1:1000 for 1 hour at RT with shaking . Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~38.9kDa. Other Band(s): Band appears at ~75 kDa indicating detection of the Rhodopsin dimer.Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody (NBP2-59690) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS  inner segment; ONL  outer nuclear layer; OPL  outer plexiform layer; INL  inner nuclear layer; IPL  inner plexiform layer; GCL  ganglion cell layer..

Mouse Monoclonal
Species Amphibian, Avian, Fish
Applications WB, ELISA, ICC/IF

     1 Review

6 Publications
NLS261
Immunohistochemistry-Paraffin: PAR3/Thrombin Receptor-like 2 Antibody [NLS261] - Pancreas, carcinomaImmunohistochemistry-Paraffin: PAR3/Thrombin Receptor-like 2 Antibody [NLS261] - Analysis of anti-PAR3 antibody with human breast.

Rabbit Polyclonal
Species Human
Applications WB (-), IHC, IHC-P

NB100-91273
Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC

     1 Review

5 Publications
NB100-116
Western Blot: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-APE-1 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.Knockdown Validated: APE Antibody (13B8E5C2) - BSA Free [NB100-116] - APE Antibody (13B8E5C2) [NB100-116] - Repression of p21 by APE1 in p53-null cells and effect of ectopic p53 in this repression. Representative Western analysis of p53, APE1, p21 and alpha-Tubulin levels in the same HCT116p53null cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0068467) licensed under a CC-BY license.

Mouse Monoclonal
Species Human, Mouse, Rat
Applications WB, Simple Western, ELISA

     3 Reviews

107 Publications
NBP2-81883
Western Blot: SHROOM3 Antibody [NBP2-81883] - Western blot analysis of SHROOM3 in SK-N-SH cell lysate with SHROOM3 antibody at 1 ug/mL.

Rabbit Polyclonal
Species Human
Applications WB, ELISA

NBP2-92546
Western Blot: DSPP Antibody [NBP2-92546] -  Analysis of lysates from wild type (WT) and 293F cells transfected with DSPP using DSPP Rabbit pAb at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution.Lysates/proteins: 40.5 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit. Exposuretime: 10s.ICC/IF:DSPP Antibody [NBP2-92546] -  Analysis of Dental pulp stem cell using DSPP Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Rabbit Polyclonal
Species Human, Mouse
Applications WB, ELISA, ICC/IF

3 Publications