Hu,
Mu,
Rt,
Po,
Ca,
Ch,
ChHa,
Fe,
Ma-Op,
Pm,
Am,
Bv,
Pm,
Pm-Or,
Re,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Fe, Ma-Op, Pm, Am, Bv, Pm, Pm-Or, Re, Ze
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Host: Rabbit Polyclonal
Alt Catalog# IMG-6796A
Sample Size Available
Sizes: Sample Size Available
![Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27202-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27202] - analysis of 14-3-3 using 14-3-3 antibody. Human Brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain, and 4) rat brain lysate probed with 2 ug/ml of 14-3-3 antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 Antibody [NBP2-27202] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-14-3-3 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-27202-img0004.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0002.jpg)
![Simple Western: 14-3-3 Antibody [NBP2-27202] - Analysis in mouse brain lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27202-img0003.jpg)
![Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : MDA-MB-21 cell lysateLane 2. : HeLa cell lysateLane 3. : A431 cell lysateLane 4. : NIH3T3 cell lysateLane 5. : 293T cell lysateLane 6. : HepG2 cell lysate](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-1A1-Western-Blot-NBP1-21301-img0006.jpg "Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : MDA-MB-21 cell lysateLane 2. : HeLa cell lysateLane 3. : A431 cell lysateLane 4. : NIH3T3 cell lysateLane 5. : 293T cell lysateLane 6. : HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - Analysis in HeLa cells line, stained with DAPI (Blue) for nucleus staining and 1:100 with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-1A1-Immunocytochemistry-Immunofluorescence-NBP1-21301-img0003.jpg)
![Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - 14-3-3 tau/theta Antibody (1A1) Cell lysates of Jurkat (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human 14-3-3 theta (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-1A1-Western-Blot-NBP1-21301-img0002.jpg)
![Western Blot: 14-3-3 tau/theta Antibody (1A1) [NBP1-21301] - The recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PDVF membrane and probed with antihuman 14-3-3 tau antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1. : Recombinant Human YWHAZLane 2. : Recombinant Human YWHABLane 3. : Recombinant Human YWHAELane 4. : Recombinant Human YWHAHLane 5. : Recombinant Human YWHAGLane 6. : Recombinant Human SFNLane 7. : Recombinant Human YWHAQ (14-3-3 tau/theta)](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-1A1-Western-Blot-NBP1-21301-img0005.jpg)
![Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NBP2-27370-img0001.jpg "Western Blot: 14-3-3 Antibody [NBP2-27370] - Analysis of Human brain tissue lysate in the 1) absence and 2) presence of immunizing peptide, and 3) A375 cell lysate probed with 2 ug/ml of 14-3-3 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: 14-3-3 Antibody [NBP2-27370] - Simple Western lane view shows a specific band for 14-3-3 Gamma in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Simple-Western-NBP2-27370-img0002.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-31537-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample (20 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:30000.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-31537-img0003.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample(30 ug whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 12% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-31537-img0004.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-31537] - Sample (30 ug of whole cell lysate) A: zebrafish eye B: zebrafish heart 12% SDS PAGE NBP1-31537 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-31537_rabbit-polyclonal-14-3-3-tau-theta-antibody-91020242021915.jpg)
![Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-31537] - 14-3-3 tau/theta antibody detects 14-3-3 tau/theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. 14-3-3 tau/theta antibody (NBP1-31537) diluted at 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/nbp1-31537_rabbit-polyclonal-14-3-3-tau-theta-antibody-91020242041235.jpg)
![Immunohistochemistry: 14-3-3 Antibody (SR1457) [NBP3-21694] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21694.](http://images.novusbio.com/fullsize/nbp3-21694_rabbit-14-3-3-mab-sr1457-6720239533741.jpg "Immunohistochemistry: 14-3-3 Antibody (SR1457) [NBP3-21694] - Immunohistochemical analysis of paraffin-embedded human breast cancer, using NBP3-21694.")
![Western Blot: 14-3-3 Antibody (SR1457) [NBP3-21694] - Western blot analysis using NBP3-21694 in Hela lysate.](http://images.novusbio.com/fullsize/nbp3-21694_rabbit-14-3-3-mab-sr1457-57202316582182.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-90337-img0005.jpg "Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: 14-3-3 tau/theta Antibody [NBP1-90337] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Immunohistochemistry-Paraffin-NBP1-90337-img0001.jpg)
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-90337] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-90337-img0003.jpg)
![Western Blot: 14-3-3 tau/theta Antibody (9G6M4) [NBP3-16722] - Western blot analysis of extracts of various cell lines, using 14-3-3 tau/theta Rabbit mAb (NBP3-16722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-9G6M4-Western-Blot-NBP3-16722-img0002.jpg "Western Blot: 14-3-3 tau/theta Antibody (9G6M4) [NBP3-16722] - Western blot analysis of extracts of various cell lines, using 14-3-3 tau/theta Rabbit mAb (NBP3-16722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: 14-3-3 tau/theta Antibody (9G6M4) [NBP3-16722] - Immunofluorescence analysis of NIH-3T3 cells using 14-3-3 tau/theta Rabbit mAb (NBP3-16722) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-9G6M4-Immunocytochemistry-Immunofluorescence-NBP3-16722-img0001.jpg)
![Western Blot: 14-3-3 Antibody [NB100-2140] - Analysis of 14-3-3 expression in Hela (A) and Jurkat (B) whole cell lysates.](http://images.novusbio.com/fullsize/14-3-3-Antibody-Western-Blot-NB100-2140-img0001.jpg "Western Blot: 14-3-3 Antibody [NB100-2140] - Analysis of 14-3-3 expression in Hela (A) and Jurkat (B) whole cell lysates.")
![Western Blot: 14-3-3 tau/theta Antibody [NBP1-06008] - (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/14-3-3-tau-theta-Antibody-Western-Blot-NBP1-06008-img0002.jpg "Western Blot: 14-3-3 tau/theta Antibody [NBP1-06008] - (0.03ug/ml) staining of Human Brain (Hippocampus) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")