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![Western Blot: WWOX Antibody [NBP2-47579] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-47579-img0005.jpg "Western Blot: WWOX Antibody [NBP2-47579] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue.")
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-47579] - Staining of human cell line MCF7 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47579-img0006.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0013.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human thyroid gland shows strong cytoplasmic postivity in glandular cells.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0008.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Stainin gof human testis shows moderate cytoplaSmic postivity in Leydig cells.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0009.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human skeletal muscle shows no postivity in myoctyes as expected.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0010.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human kidney shows moderate cytoplasmic postivity in cells in tubules.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0011.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-47579] - Staining of human cerebellum shows weak to moderate cytoplasmic positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-47579-img0007.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-47579] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-47579-img0012.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0007.jpg "Western Blot: WWOX Antibody [NBP1-33456] - Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody diluted at 1:10000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP1-33456] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33456-img0003.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. WWOX antibody dilution: 1:500.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP1-33456-img0009.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0004.jpg)
![Western Blot: WWOX Antibody [NBP1-33456] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP1-33456-img0005.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP1-33456] - WWOX antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. WWOX antibody dilution: 1:100.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP1-33456-img0008.jpg)
![Immunoprecipitation: WWOX Antibody [NBP1-33456] - WWOX antibody immunoprecipitates WWOX protein in IP experiments. IP samples: 293T whole cell extract. A. Control with 4 µg of preimmune Rabbit IgG. B. Immunoprecipitation of WWOX protein by 4 µg WWOX antibody. 10 % SDS-PAGE. The immunoprecipitated WWOX protein was detected by WWOX antibody diluted at 1:500.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP1-33456-img0006.jpg)
![Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-97814-img0003.jpg "Western Blot: WWOX Antibody [NBP2-97814] - Anti-WWOX rabbit polyclonal antibody at 1:500 dilution. Lane A: Raji Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 47 kDa")
![Immunocytochemistry/Immunofluorescence: WWOX Antibody [NBP2-97814] - Immunofluorescence staining of WWOX in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human WWOX polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97814-img0002.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody [NBP2-97814] - Immunochemical staining of human WWOX in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-Paraffin-NBP2-97814-img0004.jpg)
![Immunoprecipitation: WWOX Antibody [NBP2-97814] - WWOX was immunoprecipitated using: Lane A: 0.5 mg U251MG Whole Cell Lysate 4 ul anti-WWOX rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-WWOX rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 43 kDa](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunoprecipitation-NBP2-97814-img0001.jpg)
![Western Blot: WWOX Antibody [NBP2-32121] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32121 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody NBP2-32121. For blotting immunoprecipitated WWOX, NBP2-32121 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-32121-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: WWOX Antibody [NBP2-32121] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32121 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody NBP2-32121. For blotting immunoprecipitated WWOX, NBP2-32121 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: WWOX Antibody [NBP2-32121] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32121-img0004.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-32121] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32121-img0003.jpg)
![Western Blot: WWOX Antibody [NBP2-32122] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32122 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody. For blotting immunoprecipitated WWOX, NBP2-32122 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/WWOX-Antibody-Western-Blot-NBP2-32122-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: WWOX Antibody [NBP2-32122] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-WWOX antibody NBP2-32122 used for IP at 6 ug/mg lysate. WWOX was also immunoprecipitated by rabbit anti-WWOX antibody. For blotting immunoprecipitated WWOX, NBP2-32122 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: WWOX Antibody [NBP2-32122] - Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32122-img0003.jpg)
![Immunohistochemistry: WWOX Antibody [NBP2-32122] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- WWOX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/WWOX-Antibody-Immunohistochemistry-NBP2-32122-img0002.jpg)
![Immunohistochemistry-Paraffin: WWOX Antibody (SR2547) [NBP3-27813] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.](http://images.novusbio.com/fullsize/nbp3-27813_rabbit-wwox-mab-sr2547-75202410151110.jpg "Immunohistochemistry-Paraffin: WWOX Antibody (SR2547) [NBP3-27813] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.")
![Western Blot: WWOX Antibody (SR2547) [NBP3-27813] - Western blot analysis of WWOX in Hela, U2OS, U251MG lysates](http://images.novusbio.com/fullsize/nbp3-27813_rabbit-wwox-mab-sr2547-752024936360.jpg)
![Western Blot: WWOX Antibody (SR2547) [NBP3-27813] - Western blot analysis of WWOX in mouse testis, mouse spleen lysates](http://images.novusbio.com/fullsize/nbp3-27813_rabbit-wwox-mab-sr2547-75202410133488.jpg)
![Western Blot: WWOX Overexpression Lysate (Adult Normal) [NBL1-17891] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WWOX.](http://images.novusbio.com/fullsize/WWOX-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17891-img0002.jpg "Western Blot: WWOX Overexpression Lysate (Adult Normal) [NBL1-17891] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for WWOX.")
![SDS-Page: WWOX Protein [NBC1-28766] - WWOX, 28.3 kDa (254aa), confirmed by MALDI-TOF with a purity of 85% by SDS - PAGE](http://images.novusbio.com/fullsize/WWOX-Protein-SDS-Page-NBC1-28766-img0001.jpg "SDS-Page: WWOX Protein [NBC1-28766] - WWOX, 28.3 kDa (254aa), confirmed by MALDI-TOF with a purity of 85% by SDS - PAGE")