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![Western Blot: TAF15 Antibody [NB100-567] - ALS proteins associate with the RNAP II/U1 snRNP machinery in an RNA-independent manner. IPs were carried out from RNase A-treated or untreated nuclear extract using an RNAP II or a negative control antibody (EIF4A3) followed by westerns with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article/46/22/11939/5162471), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TAF15-Antibody-Western-Blot-NB100-567-img0007.jpg "Western Blot: TAF15 Antibody [NB100-567] - ALS proteins associate with the RNAP II/U1 snRNP machinery in an RNA-independent manner. IPs were carried out from RNase A-treated or untreated nuclear extract using an RNAP II or a negative control antibody (EIF4A3) followed by westerns with the indicated antibodies. Image collected and cropped by CiteAb from the following publication (//academic.oup.com/nar/article/46/22/11939/5162471), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-567] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NB100-567-img0006.jpg)
![Immunoprecipitation: TAF15 Antibody [NB100-567] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-567. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunoprecipitation-NB100-567-img0002.jpg)
![Western Blot: TAF15 Antibody [NB100-567] - FET proteins & MATR3 associate with U1 snRNP. (a) Immunoprecipitations (IPs) were carried out with antibodies to FET proteins or MATR3 followed by analysis on a Coomassie-stained gel. Molecular weight markers & protein identified by mass spectrometry are indicated. (b) IPs were carried out from nuclear extract using a negative control antibody (EIF4A3) or an antibody to the SNRPC subunit of the U1 snRNP followed by Westerns with the indicated antibodies. (c) IPs were carried out with the indicated antibodies from nuclear extract treated with a U1 snRNA AMO or a negative control AMO followed by Western using the SNRPC antibody. (d) Same as (c) except that total RNAs from the IPs were examined on a denaturing gel stained with ethidium bromide. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29884807), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-567_rabbit-polyclonal-taf15-antibody-310202415304282.jpg)
![Simple Western: TAF15 Antibody [NB100-567] - Autophagic clearance of aberrantly accumulated cytoplasmic FUS restores protein homeostasis & ameliorates survival of SL P525L iPSC-derived neurons. a Confocal micrographs showing FUS-eGFP distribution before & after Torkinib treatment (above). Arrowhead indicates FUS-eGFP cytoplasmic accumulation in untreated neurites; arrow shows reduced FUS-eGFP cytoplasmic signal following torkinib treatment. Quantification of cytoplasmic FUS-eGFP signal intensity in acquired images (below) confirms clearance of mislocalized FUS-eGFP protein. Scale bar = 10 µm. b FRAP analysis performed on untreated versus torkinib-treated neurons shows comparable dynamics of FUS-eGFP recovery. n = 3. Error bars indicate SEM. CHX = cycloheximide. c WES capillary electrophoresis & d corresponding quantification of the indicated proteins in P525L SL neurons before & after torkinib treatment. Autophagy stimulation restores physiological levels. n = 4. Error bars indicate SEM. * & ** Correspond to p < 0.05 & 0.01, respectively. e 6 h of torkinib reduces apoptotic cell death identified by cleaved Caspase 3 staining. Scale bar = 50 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-567_rabbit-polyclonal-taf15-antibody-3102024165740.jpg)
![Simple Western: TAF15 Antibody [NB100-567] - The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b Western blot analysis of FUS protein interactors in a LL & b SL neurons after FUS-eGFP immunoprecipitation reveals differential interactions with several ALS-associated partners. n = 4. Error bars indicate SEM. *, **, & *** Correspond to p < 0.05, 0.01, & 0.001, respectively. c In vitro phase separation assay showing fibrillization of purified P525L LL FUS-eGFP protein in the presence or absence of distinct RBPs. Investigated RBPs effectively prevent FUS fibril formation. d Fluorescence recovery after photobleaching (FRAP) was used to assess the dynamics of P525L LL FUS at the tested conditions for the indicated time points. RBPs promote the maintenance of a liquid-like behavior. e Co-localization of P525L LL FUS with the reported RBPs. Scale bar 5 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-567_rabbit-polyclonal-taf15-antibody-31020241683924.jpg)
![Simple Western: TAF15 Antibody [NB100-567] - The cytoplasmic mislocalization induced by P525L causes reduced FUS binding to several ALS-associated RBPs, promoting aggregation. a, b Western blot analysis of FUS protein interactors in a LL & b SL neurons after FUS-eGFP immunoprecipitation reveals differential interactions with several ALS-associated partners. n = 4. Error bars indicate SEM. *, **, & *** Correspond to p < 0.05, 0.01, & 0.001, respectively. c In vitro phase separation assay showing fibrillization of purified P525L LL FUS-eGFP protein in the presence or absence of distinct RBPs. Investigated RBPs effectively prevent FUS fibril formation. d Fluorescence recovery after photobleaching (FRAP) was used to assess the dynamics of P525L LL FUS at the tested conditions for the indicated time points. RBPs promote the maintenance of a liquid-like behavior. e Co-localization of P525L LL FUS with the reported RBPs. Scale bar 5 µm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30937520), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-567_rabbit-polyclonal-taf15-antibody-310202415541943.jpg)
![Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.](http://images.novusbio.com/fullsize/TAF15-Antibody-4D71-Western-Blot-NBP1-92685-img0002.jpg "Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.")
![Immunocytochemistry/Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, NBP1-92685, dilution 1:500 in red, and costained with rabbit pAb to GAPDH, dilution 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The NBP1-92685 antibody reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.](http://images.novusbio.com/fullsize/TAF15-Antibody-4D71-Immunocytochemistry-Immunofluorescence-NBP1-92685-img0001.jpg)
![Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-NB100-566-img0003.jpg "Immunohistochemistry-Paraffin: TAF15 Antibody [NB100-566] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TAFII68 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunoprecipitation: TAF15 Antibody [NB100-566] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-566. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunoprecipitation-NB100-566-img0001.jpg)
![Western Blot: TAF15 Antibody [NBP1-32461] - (30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TAF15-Antibody-Western-Blot-NBP1-32461-img0002.jpg "Western Blot: TAF15 Antibody [NBP1-32461] - (30 ug whole cell lysate) A:Raji 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: TAF15 Antibody [NBP1-32461] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32461-img0006.jpg)
![Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461] - Paraffin-embedded human breast carcinoma. TAFII68 antibody [N1N3] diluted at 1:250.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NBP1-32461-img0007.jpg)
![Western Blot: TAF15 Antibody [NBP1-32461] - (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TAF15-Antibody-Western-Blot-NBP1-32461-img0001.jpg)
![Immunohistochemistry-Paraffin: TAF15 Antibody [NBP1-32461] - Paraffin-embedded CL1-0 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NBP1-32461-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: TAF15 Antibody [NBP2-13410] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13410-img0008.jpg "Immunocytochemistry/Immunofluorescence: TAF15 Antibody [NBP2-13410] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining in human testis and pancreas tissues using anti-TAF15 antibody. Corresponding TAF15 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NBP2-13410-img0007.jpg)
![Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NBP2-13410-img0001.jpg)
![Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NBP2-13410-img0005.jpg)
![Immunohistochemistry-Paraffin: TAF15 Antibody [NBP2-13410] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunohistochemistry-Paraffin-NBP2-13410-img0006.jpg)
![Western Blot: TAF15 Antibody (SR2205) [NBP3-22496] - Western blot analysis using NBP3-22496 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22496_rabbit-taf15-mab-sr2205-5720231754857.jpg "Western Blot: TAF15 Antibody (SR2205) [NBP3-22496] - Western blot analysis using NBP3-22496 in HeLa cell lysate.")
![Western Blot: TAF15 Antibody [NB100-568] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAFII68 antibody A used for WB at 0.033 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TAF15-Antibody-Western-Blot-NB100-568-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: TAF15 Antibody [NB100-568] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TAFII68 antibody A used for WB at 0.033 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: TAF15 Antibody [NB100-568] - Detection of human TAFII68 on HeLa whole cell lysate using NB100-568. TAFII68 was IPed with rabbit anti-TAFII68 antibodies NB100-566, NB100-567, NB100-568, and another using each at 0.3 ug/mg lysate.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunoprecipitation-NB100-568-img0001.jpg)
![Western Blot: TAF15 Antibody [NBP2-57720] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/TAF15-Antibody-Western-Blot-NBP2-57720-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: TAF15 Antibody [NBP2-57720] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: TAF15 Antibody [NBP2-57720] - Staining of human cell line SiHa shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/TAF15-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57720-img0001.jpg)
![SDS-Page: TAF15 Protein [NBP2-22848]](http://images.novusbio.com/fullsize/TAF15-Protein-SDS-Page-NBP2-22848-img0001.jpg "SDS-Page: TAF15 Protein [NBP2-22848]")