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![Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP1-32728] - Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32728-img0003.jpg "Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP1-32728] - Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37C for 30 min. Green: SUCLG1 protein stained by SUCLG1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-32728] - N87 xenograft, using SUCLG1antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-32728-img0004.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-32728] - Sample (20 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP1-32728-img0005.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-32728] - 50 ug rat brain lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:3000.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP1-32728-img0006.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-32728] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody (NBP1-32728) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32728_rabbit-polyclonal-suclg1-antibody-91020242024618.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-89489] - Analysis using Anti-SUCLG1 antibody NBP1-89489 (A) shows similar pattern to independent antibody NBP2-38318 (B).](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP1-89489-img0014.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SUCLG1 Antibody [NBP1-89489] - Analysis using Anti-SUCLG1 antibody NBP1-89489 (A) shows similar pattern to independent antibody NBP2-38318 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP1-89489] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89489-img0009.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0022.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-89489] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP1-89489-img0006.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-89489] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP1-89489-img0007.jpg)
![Western Blot: SUCLG1 Antibody [NBP1-89489] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP1-89489-img0008.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0010.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining in human kidney and pancreas tissues using anti-SUCLG1 antibody. Corresponding SUCLG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human cerebral cortex, colon, kidney and pancreas using Anti-SUCLG1 antibody NBP1-89489 (A) shows similar protein distribution across tissues to independent antibody NBP2-38318 (B).](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0013.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0015.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human colon.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0016.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human cerebellum shows strong granular cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human cerebellum, fallopian tube, kidney and stomach using Anti-SUCLG1 antibody NBP1-89489 (A) shows similar protein distribution across tissues to independent antibody NBP2-38318 (B).](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0019.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0020.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP1-89489] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP1-89489-img0021.jpg)
![Western Blot: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249355546.jpg "Western Blot: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. SUCLG1 stained by SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249415995.jpg)
![Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: SUCLG1 stained by SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249355533.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. SUCLG1 stained by SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249403894.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - SUCLG1 antibody [HL2387] detects SUCLG1 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded rat tissue. SUCLG1 stained by SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249342715.jpg)
![Western Blot: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249425446.jpg)
![Western Blot: SUCLG1 Antibody (HL2387) - Azide and BSA Free [NBP3-25700] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SUCLG1 antibody [HL2387] (NBP3-25700) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25700_rabbit-suclg1-mab-hl2387-azide-and-bsa-free-2120249371548.jpg)
![Western Blot: SUCLG1 Antibody [NBP2-38318] - Analysis using Anti-SUCLG1 antibody NBP2-38318 (A) shows similar pattern to independent antibody NBP1-89489 (B).](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP2-38318-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: SUCLG1 Antibody [NBP2-38318] - Analysis using Anti-SUCLG1 antibody NBP2-38318 (A) shows similar pattern to independent antibody NBP1-89489 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0015.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0004.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining in human kidney and pancreas tissues using anti-SUCLG1 antibody. Corresponding SUCLG1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0005.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0008.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human colon.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0009.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human cerebellum shows strong granular cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human cerebellum, fallopian tube, kidney and stomach using Anti-SUCLG1 antibody NBP2-38318 (A) shows similar protein distribution across tissues to independent antibody NBP1-89489 (B).](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0012.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human Fallopian tube shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0013.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-38318] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-38318-img0014.jpg)
![Western Blot: SUCLG1 Antibody [NBP2-93948] - Analysis of extracts of various cell lines, using SUCLG1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP2-93948-img0002.jpg "Western Blot: SUCLG1 Antibody [NBP2-93948] - Analysis of extracts of various cell lines, using SUCLG1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP2-93948] - Immunofluorescence analysis of C6 cells using SUCLG1 antibody (NBP2-93948) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93948-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP2-93948] - Analysis of L929 cells using SUCLG1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93948-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP2-97325] - Immunofluorescence staining of SUCLG1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SUCLG1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97325-img0001.jpg "Immunocytochemistry/Immunofluorescence: SUCLG1 Antibody [NBP2-97325] - Immunofluorescence staining of SUCLG1 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SUCLG1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.")
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-97325] - Immunochemical staining of human SUCLG1 in human stomach with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-97325-img0003.jpg)
![Immunohistochemistry-Paraffin: SUCLG1 Antibody [NBP2-97325] - Immunochemical staining of human SUCLG1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Immunohistochemistry-Paraffin-NBP2-97325-img0002.jpg)
![Western Blot: SUCLG1 Antibody [NBP2-88377] - Host: Rabbit. Target Name: SUCLG1. Sample Tissue: Human HCT116 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP2-88377-img0001.jpg "Western Blot: SUCLG1 Antibody [NBP2-88377] - Host: Rabbit. Target Name: SUCLG1. Sample Tissue: Human HCT116 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: SUCLG1 Antibody [NBP2-88375] - WB Suggested Anti-SUCLG1 Antibody. Titration: 1.0 ug/ml. Positive Control: U937 Whole Cell](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP2-88375-img0001.jpg "Western Blot: SUCLG1 Antibody [NBP2-88375] - WB Suggested Anti-SUCLG1 Antibody. Titration: 1.0 ug/ml. Positive Control: U937 Whole Cell")
![Western Blot: SUCLG1 Antibody [NBP2-88376] - Host: Rabbit. Target Name: SUCLG1. Antibody Dilution: 1.0ug/ml. Sample Type: Human Lung](http://images.novusbio.com/fullsize/SUCLG1-Antibody-Western-Blot-NBP2-88376-img0001.jpg "Western Blot: SUCLG1 Antibody [NBP2-88376] - Host: Rabbit. Target Name: SUCLG1. Antibody Dilution: 1.0ug/ml. Sample Type: Human Lung")
![Western Blot: SUCLG1 Overexpression Lysate (Adult Normal) [NBP2-07943] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SUCLG1.](http://images.novusbio.com/fullsize/SUCLG1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07943-img0001.jpg "Western Blot: SUCLG1 Overexpression Lysate (Adult Normal) [NBP2-07943] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SUCLG1.")