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![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6. Analysis of SPHK1 expression in K-562.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-Western-Blot-H00008877-M01-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6. Analysis of SPHK1 expression in K-562.")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - SPHK1 monoclonal antibody (M01), clone 1D6 Western Blot analysis of SPHK1 expression in A-431 ( Cat # L015V1 ).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-Western-Blot-H00008877-M01-img0002.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - Analysis of SPHK1 expression in transfected 293T cell line by SPHK1 monoclonal antibody (M01), clone 1D6.Lane 1: SPHK1 transfected lysate(43.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-Western-Blot-H00008877-M01-img0006.jpg)
![ELISA: Sphingosine Kinase 1/SPHK1 Antibody (1D6) [H00008877-M01] - Detection limit for recombinant GST tagged SPHK1 is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1D6-ELISA-H00008877-M01-img0007.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1A6-Western-Blot-NBP2-45944-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1A6-Immunofluorescence-NBP2-45944-img0002.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1A6-Immunohistochemistry-NBP2-45944-img0004.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1A6-Western-Blot-NBP2-45944-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1A6-Immunofluorescence-NBP2-45944-img0001.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-1A6-Immunohistochemistry-NBP2-45944-img0003.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0004.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0005.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Western-Blot-NBP2-74332-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74332-img0003.jpg)
![Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) - Azide and BSA Free [NBP2-74332] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-OTI1A6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74332-img0006.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0005.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Western blot analysis of Sphingosine Kinase 1/SPHK1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: MCF-7 cell lysate")
![Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of paraffin-embedded human kidney tissue using anti-SPHK1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Immunohistochemistry-Paraffin-NBP2-67164-img0002.jpg)
![Flow Cytometry: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of Raji cells with SPHK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Flow-Cytometry-NBP2-67164-img0001.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (JA31-14) [NBP2-67164] - Analysis of SPHK1 on C2C12 lysates using anti-SPHK1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-JA31-14-Western-Blot-NBP2-67164-img0004.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-Western-Blot-NBP1-22974-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope")
![Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449] - Analysis of anti-SPHK1 antibody with human adrenal, pheochromocytes at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB110-40449-img0001.jpg "Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449] - Analysis of anti-SPHK1 antibody with human adrenal, pheochromocytes at concentration 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20472-img0003.jpg "Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Sphingosine Kinase 1/SPHK1 antibody (NBP2-20472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20472_rabbit-polyclonal-sphingosine-kinase-1-sphk1-antibody-9102024205622.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23419_rat-sphingosine-kinase-1-sphk1-mab-m540-199202312523633.jpg "Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23419_rat-sphingosine-kinase-1-sphk1-mab-m540-19920231856961.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-30856] - Western blot analysis using Sphingosine Kinase 1/SPHK1 antibody at a dilution of 1:400 on MDBK cell lysate using Novus Biologicals' mouse anti-rabbit HRP secondary antibody at 1:75,000.](http://images.novusbio.com/fullsize/nbp3-30856_rabbit-sphingosine-kinase-1-sphk1-pab-81120242256271.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP3-30856] - Western blot analysis using Sphingosine Kinase 1/SPHK1 antibody at a dilution of 1:400 on MDBK cell lysate using Novus Biologicals' mouse anti-rabbit HRP secondary antibody at 1:75,000.")