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![Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunocytochemistry-Immunofluorescence-NBP2-61421-img0001.jpg "Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).")
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human endometrium shows moderate to strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of human liver shows absence of nuclear positivity in hepatocytes as expected (negative control).](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse forebrain shows strong positivity in cells in the rostral migratory stream, as well as in a subset of neurons in the olfactory bulb.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse forebrain shows strong immunoreactivity in cells in the rostral migratory stream.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0005.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of mouse hippocampus shows strong immunoreactivity in a subset of neurons in the granule cell layer of the dentate gyrus.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5665) [NBP2-61421] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5665-Immunohistochemistry-Paraffin-NBP2-61421-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunocytochemistry-Immunofluorescence-NBP2-61420-img0001.jpg "Immunocytochemistry/Immunofluorescence: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of SH-SY5Y cells using the Anti-SOX4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of rat hippocampus shows strong nuclear immunoreactivity in a subset of cells in the granule cell layer of the dentate gyrus.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunohistochemistry-Paraffin-NBP2-61420-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunohistochemistry-Paraffin-NBP2-61420-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of human liver shows absence of nuclear positivity in hepatocytes as expected (negative control).](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunohistochemistry-Paraffin-NBP2-61420-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of mouse forebrain shows strong positivity in cells in the rostral migratory stream, as well as in a subset of neurons in the olfactory bulb.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunohistochemistry-Paraffin-NBP2-61420-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of mouse forebrain shows strong immunoreactivity in cells in the rostral migratory stream.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunohistochemistry-Paraffin-NBP2-61420-img0005.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (CL5634) [NBP2-61420] - Staining of mouse hippocampus shows strong immunoreactivity in a subset of neurons in the granule cell layer of the dentate gyrus.](http://images.novusbio.com/fullsize/SOX4-Antibody-CL5634-Immunohistochemistry-Paraffin-NBP2-61420-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).](http://images.novusbio.com/fullsize/SOX4-Antibody-PCRP-SOX4-1D6-Immunocytochemistry-Immunofluorescence-NBP3-13777-img0002.jpg "Immunocytochemistry/Immunofluorescence: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).")
![Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SOX4-Antibody-PCRP-SOX4-1D6-Immunohistochemistry-Paraffin-NBP3-13777-img0004.jpg)
![Flow Cytometry: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Flow Cytometric Analysis of PFA-fixed HeLa cells. SOX4 antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).](http://images.novusbio.com/fullsize/SOX4-Antibody-PCRP-SOX4-1D6-Flow-Cytometry-NBP3-13777-img0001.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with SOX4 antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/SOX4-Antibody-PCRP-SOX4-1D6-Immunohistochemistry-Paraffin-NBP3-13777-img0003.jpg)
![Protein Array: SOX4 Antibody (PCRP-SOX4-1D6) [NBP3-13777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX4 Antibody (PCRP-SOX4-1D6).](http://images.novusbio.com/fullsize/SOX4-Antibody-PCRP-SOX4-1D6-Protein-Array-NBP3-13777-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: SOX4 Antibody [NBP1-89506] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SOX4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89506-img0005.jpg "Immunocytochemistry/Immunofluorescence: SOX4 Antibody [NBP1-89506] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506] - Staining of human pancreas shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/SOX4-Antibody-Immunohistochemistry-Paraffin-NBP1-89506-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/SOX4-Antibody-Immunohistochemistry-Paraffin-NBP1-89506-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.](http://images.novusbio.com/fullsize/SOX4-Antibody-Immunohistochemistry-Paraffin-NBP1-89506-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody [NBP1-89506] - Staining of human uterine cervix shows moderate nuclear positivity in a subset of epidermal cells.](http://images.novusbio.com/fullsize/SOX4-Antibody-Immunohistochemistry-Paraffin-NBP1-89506-img0010.jpg)
![Western Blot: SOX4 Antibody [NBP3-18643] - Figure 4. IP using the antibody directed against SOX4 IP was performed overnight on protein extracts (600 ug) from CT-2A (A) and GL261 (B) cells using 6 ul of the antibody against SOX4 . The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-NBP3-18643-img0003.jpg "Western Blot: SOX4 Antibody [NBP3-18643] - Figure 4. IP using the antibody directed against SOX4 IP was performed overnight on protein extracts (600 ug) from CT-2A (A) and GL261 (B) cells using 6 ul of the antibody against SOX4 . The immunoprecipitated proteins were subsequently analysed by Western blot with the antibody as described above. Lane 2 shows the result of the IP; the input is shown in lane 1.")
![Western Blot: SOX4 Antibody [NBP3-18643] - Figure 2. Western blot analysis using the antibody directed against SOX4 A. H1299 lung carcinoma cells were transfected with HA-tagged SOX4 (lane 1) or with a HA-tagged truncated SOX4 (395X mutant, lane 2). Whole cell extracts (60 - 100 ug) were analysed by Western blot using the antibody against SOX4 diluted 1:4,000 in TBS-Tween containing 2% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left. B. Whole cell extracts of 4 different lung carcinoma cell lines were analysed by Western blot with the antibody against SOX4 as described above. The H522 cells are known to overexpress SOX4. Western blot performed by Montse Sanchez-Cespedes and Sandra Castillo Diez, Catalan Institute of Oncology, Barcelona, Spain.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-NBP3-18643-img0001.jpg)
![Western Blot: SOX4 Antibody [NBP3-18643] - Figure 3. Western blot analysis using the antibody directed against SOX4 Western blot was performed on Protein extracts (~40 ug) from mouse B16-OVA (A), CT-2A (B), GL261 (C) or B16A (D) cells using the antibody against SOX4 . The cells were transfected with a SOX4 specific crRNA (lanes 2) or with a negative control crRNA (lanes 1). The antibody was diluted 1:4,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right. The lower panel shows the WB results with a GAPDH antibody used as loading control. Western blot performed by Anze Godicelj, Dana Farber Cancer Institute, Boston, USA.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-NBP3-18643-img0002.jpg)
![ELISA: SOX4 Antibody [NBP3-18643] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human SOX4 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:18,000.](http://images.novusbio.com/fullsize/SOX4 Antibody-ELISA-NBP3-18643-img0004.jpg)
![Immunohistochemistry: SOX4 Antibody [NBP3-27897] - Immunohistochemistry of SOX4 in rat testis tissue with SOX4 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp3-27897_rabbit-sox4-pab-271120242022939.jpg "Immunohistochemistry: SOX4 Antibody [NBP3-27897] - Immunohistochemistry of SOX4 in rat testis tissue with SOX4 antibody at 5 u/mL.")
![Western Blot: SOX4 Antibody [NBP3-27897] - Western blot analysis of SOX4 in human testis tissue lysate with SOX4 antibody at 1 u/ml.](http://images.novusbio.com/fullsize/nbp3-27897_rabbit-sox4-pab-2711202419201583.gif)
![Immunocytochemistry/ Immunofluorescence: SOX4 Antibody [NBP3-27897] - Immunofluorescence of SOX4 in rat testis tissue with SOX4 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp3-27897_rabbit-sox4-pab-2711202419432239.jpg)
![Immunohistochemistry-Paraffin: SOX4 Antibody (SOX4/2540) [NBP2-79768] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SOX4 Antibody (SOX4/2540).](http://images.novusbio.com/fullsize/SOX4-Antibody-SOX4-2540-Immunohistochemistry-Paraffin-NBP2-79768-img0002.jpg "Immunohistochemistry-Paraffin: SOX4 Antibody (SOX4/2540) [NBP2-79768] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SOX4 Antibody (SOX4/2540).")
![Immunohistochemistry-Paraffin: SOX4 Antibody (SOX4/2540) [NBP2-79768] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with SOX4 Antibody (SOX4/2540).](http://images.novusbio.com/fullsize/SOX4-Antibody-SOX4-2540-Immunohistochemistry-Paraffin-NBP2-79768-img0001.jpg)
![Protein Array: SOX4 Antibody (SOX4/2540) [NBP2-79768] - Analysis of Protein Array containing more than 19,000 full-length human proteins using SOX4 Antibody (SOX4/2540). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/SOX4-Antibody-SOX4-2540-Protein-Array-NBP2-79768-img0003.jpg)
![Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] - Western blot analysis of various lysates, using SOX4 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33358_rabbit-sox4-mab-4a1e8-912202415171151.jpg "Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] - Western blot analysis of various lysates, using SOX4 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: SOX4 Antibody (4A1E8) [NBP3-33358] - Western blot analysis of lysates from Rat lung, using SOX4 Rabbit mAb at 1:20000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33358_rabbit-sox4-mab-4a1e8-91220241411288.jpg)
![SDS-Page: Recombinant Human SOX4 Protein [H00006659-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00006659-Q01-1.jpg "SDS-Page: Recombinant Human SOX4 Protein [H00006659-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SOX4 Antibody [H00006659-A01] - WB analysis of 36.23 kDa SOX4 partial recombinant protein (NP_003098, 45 a.a. ~ 136 a.a) with GST tag using mouse polyclonal SOX4 antibody.](http://images.novusbio.com/fullsize/SOX4-Antibody-Western-Blot-H00006659-A01-img0001.jpg "Western Blot: SOX4 Antibody [H00006659-A01] - WB analysis of 36.23 kDa SOX4 partial recombinant protein (NP_003098, 45 a.a. ~ 136 a.a) with GST tag using mouse polyclonal SOX4 antibody.")
![Western Blot: SOX4 Overexpression Lysate (Adult Normal) [NBL1-16352] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOX4.](http://images.novusbio.com/fullsize/SOX4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16352-img0002.jpg "Western Blot: SOX4 Overexpression Lysate (Adult Normal) [NBL1-16352] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SOX4.")
![Sandwich ELISA: SOX4 Antibody (2C5) [H00006659-M01] - Detection limit for recombinant GST tagged SOX4 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SOX4-Antibody-2C5-Sandwich-ELISA-H00006659-M01-img0002.jpg "Sandwich ELISA: SOX4 Antibody (2C5) [H00006659-M01] - Detection limit for recombinant GST tagged SOX4 is approximately 0.3ng/ml as a capture antibody.")