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![Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunocytochemistry-Immunofluorescence-NBP2-66967-img0002.jpg "Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded rat trachea tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Staining smooth muscle Myosin heavy chain 11 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunocytochemistry-Immunofluorescence-NBP2-66967-img0001.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human colon tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0003.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human jejunum tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0004.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human prostate tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (JA03-35) [NBP2-66967] - Analysis of paraffin-embedded human stomach cancer tissue using anti-smooth muscle Myosin heavy chain 11 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-JA03-35-Immunohistochemistry-Paraffin-NBP2-66967-img0006.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Analysis in human prostate and skeletal muscle tissues. Corresponding MYH11 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87025-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Analysis in human prostate and skeletal muscle tissues. Corresponding MYH11 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human cell line U-2 OS shows positivity in nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87025-img0010.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87025-img0016.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human heart muscle shows no postivity in cardiomyocytes as expected.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87025-img0013.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human liver shows no postivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87025-img0014.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87025] - Staining of human prostate shows very strong cytoplasmic postivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87025-img0015.jpg)
![Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Mouse lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Western-Blot-NBP3-16323-img0004.jpg "Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Mouse lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunohistochemistry: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Immunofluorescence analysis of mouse colon using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Immunohistochemistry-NBP3-16323-img0003.jpg)
![Western Blot: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Western blot analysis of extracts of Rat lung, using SMMHC/Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Western-Blot-NBP3-16323-img0005.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Immunohistochemistry of paraffin-embedded human colon using Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Immunohistochemistry-Paraffin-NBP3-16323-img0001.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (8A8J4) [NBP3-16323] - Immunohistochemistry of paraffin-embedded human placenta using Myosin heavy chain 11 Rabbit mAb (NBP3-16323) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-8A8J4-Immunohistochemistry-Paraffin-NBP3-16323-img0002.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/2303R) [NBP3-07595] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody (MYH11/2303R).](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-MYH11-2303R-Immunohistochemistry-Paraffin-NBP3-07595-img0003.jpg "Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/2303R) [NBP3-07595] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody (MYH11/2303R).")
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/2303R) [NBP3-07595] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody (MYH11/2303R).](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-MYH11-2303R-Immunohistochemistry-Paraffin-NBP3-07595-img0001.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (MYH11/2303R) [NBP3-07595] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Myosin heavy chain 11 Recombinant Rabbit Monoclonal Antibody (MYH11/2303R).](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-MYH11-2303R-Immunohistochemistry-Paraffin-NBP3-07595-img0002.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87026] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87026-img0004.jpg "Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87026] - Staining of human skeletal muscle shows low expression as expected.")
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87026] - Staining of human breast shows moderate positivity in myoepithelial cells.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87026-img0002.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87026] - Staining of human prostate shows high expression.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87026-img0003.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody [NBP1-87026] - Staining in human prostate and skeletal muscle tissues using anti-MYH11 antibody. Corresponding MYH11 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Immunohistochemistry-Paraffin-NBP1-87026-img0005.jpg)
![Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221] - Nuclear extracts of ME-1 cells were analysed by Western blot using the antibodies against CBFb and MYH11 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Western-Blot-NBP2-59221-img0001.jpg "Western Blot: Myosin heavy chain 11 Antibody [NBP2-59221] - Nuclear extracts of ME-1 cells were analysed by Western blot using the antibodies against CBFb and MYH11 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the CBFb and CBFb-MYH11 fusion proteins is indicated on the right.")
![Chromatin Immunoprecipitation: Myosin heavy chain 11 Antibody [NBP2-59221] - ChIP assays were performed using ME-1 cells, the antibody against MYH11 and optimized primer pairs for qPCR. Sheared chromatin from 1.5 million cells and 5 uL of antibody were used per ChIP experiment. QPCR was performed using primers specific for the genes indicated. Figure shows the relative occupancy, calculated as the ratio + control/background for which the MYOG gene was used.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-Chromatin-Immunoprecipitation-NBP2-59221-img0003.jpg)
![ELISA: Myosin heavy chain 11 Antibody [NBP2-59221] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human MYH11. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:1,900.](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-ELISA-NBP2-59221-img0002.jpg)
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (160) [NBP2-89243] - Staining of human MYH11 in human prostate with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-160-Immunohistochemistry-Paraffin-NBP2-89243-img0002.jpg "Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (160) [NBP2-89243] - Staining of human MYH11 in human prostate with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: Myosin heavy chain 11 Antibody (160) [NBP2-89243] - Staining of human MYH11 in human smooth muscle with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Myosin-heavy-chain-11-Antibody-160-Immunohistochemistry-Paraffin-NBP2-89243-img0001.jpg)
![ELISA: Human Myosin heavy chain 11 ELISA Kit (Colorimetric) [NBP3-39536] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39536_human-myosin-heavy-chain-11-elisa-kit-colorimetric-2110202415302963.png "ELISA: Human Myosin heavy chain 11 ELISA Kit (Colorimetric) [NBP3-39536] - Standard Curve Reference")