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![Immunoprecipitation: SHARPIN Antibody [NBP2-04116] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SHARPIN antibody NBP2-04116 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SHARPIN was also immunoprecipitated by rabbit anti-SHARPIN antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/SHARPIN-Antibody-Immunoprecipitation-NBP2-04116-img0001.jpg "Immunoprecipitation: SHARPIN Antibody [NBP2-04116] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SHARPIN antibody NBP2-04116 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SHARPIN was also immunoprecipitated by rabbit anti-SHARPIN antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP2-27186] - Formalin-fixed, paraffin-embedded human brain stained with Sharpin antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.](http://images.novusbio.com/fullsize/SHARPIN-Antibody-Immunohistochemistry-Paraffin-NBP2-27186-img0001.jpg "Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP2-27186] - Formalin-fixed, paraffin-embedded human brain stained with Sharpin antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.")
![Western Blot: SHARPIN Antibody [NBP1-92386] - Analysis in human cell lines PC-3 and Caco-2 using anti-SHARPIN antibody. Corresponding SHARPIN RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.](http://images.novusbio.com/fullsize/SHARPIN-Antibody-Western-Blot-NBP1-92386-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SHARPIN Antibody [NBP1-92386] - Analysis in human cell lines PC-3 and Caco-2 using anti-SHARPIN antibody. Corresponding SHARPIN RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP1-92386] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SHARPIN-Antibody-Immunohistochemistry-Paraffin-NBP1-92386-img0006.jpg)
![Western Blot: SHARPIN Antibody [NBP1-92386] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/SHARPIN-Antibody-Western-Blot-NBP1-92386-img0005.jpg)
![Recombinant Human SHARPIN Protein [H00081858-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00081858-P01-1.jpg "Recombinant Human SHARPIN Protein [H00081858-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: SHARPIN Overexpression Lysate (Adult Normal) [NBP2-06154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SHARPIN.](http://images.novusbio.com/fullsize/SHARPIN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06154-img0001.jpg "Western Blot: SHARPIN Overexpression Lysate (Adult Normal) [NBP2-06154] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SHARPIN.")