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![Western Blot: RNF5 Antibody [NBP2-94913] - Analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/RNF5-Antibody-Western-Blot-NBP2-94913-img0004.jpg "Western Blot: RNF5 Antibody [NBP2-94913] - Analysis of extracts of various cell lines, using RNF5 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Rat liver using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunohistochemistry-Paraffin-NBP2-94913-img0003.jpg)
![Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Human liver cancer using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunohistochemistry-Paraffin-NBP2-94913-img0001.jpg)
![Immunohistochemistry-Paraffin: RNF5 Antibody [NBP2-94913] - Mouse heart using RNF5 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunohistochemistry-Paraffin-NBP2-94913-img0002.jpg)
![Immunoprecipitation: RNF5 Antibody [NBP2-04014] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF5 antibody NBP2-04014 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RNF5 was also immunoprecipitated by rabbit anti-RNF5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunoprecipitation-NBP2-04014-img0001.jpg "Immunoprecipitation: RNF5 Antibody [NBP2-04014] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNF5 antibody NBP2-04014 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. RNF5 was also immunoprecipitated by rabbit anti-RNF5 antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![Immunocytochemistry/Immunofluorescence: RNF5 Antibody [NBP2-57712] - Staining of human cell line HEK 293 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/RNF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57712-img0001.jpg "Immunocytochemistry/Immunofluorescence: RNF5 Antibody [NBP2-57712] - Staining of human cell line HEK 293 shows localization to endoplasmic reticulum.")
![Western Blot: RNF5 Antibody [NBP2-20192] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RNF5-Antibody-Western-Blot-NBP2-20192-img0001.jpg "Western Blot: RNF5 Antibody [NBP2-20192] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RNF5 Overexpression Lysate (Adult Normal) [NBP2-04322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF5.](http://images.novusbio.com/fullsize/RNF5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04322-img0001.jpg "Western Blot: RNF5 Overexpression Lysate (Adult Normal) [NBP2-04322] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RNF5.")