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![Western Blot: PSMC1 Antibody [NBP1-80959] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Western-Blot-NBP1-80959-img0010.jpg "Western Blot: PSMC1 Antibody [NBP1-80959] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.")
![Immunocytochemistry/Immunofluorescence: PSMC1 Antibody [NBP1-80959] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80959-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP1-80959] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-80959-img0015.jpg)
![Western Blot: PSMC1 Antibody [NBP1-80959] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Western-Blot-NBP1-80959-img0006.jpg)
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP1-80959] - Staining of human prostate shows moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-80959-img0012.jpg)
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP1-80959] - Staining of human skeletal muscle shows moderate nuclear postivity in myocytes.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-80959-img0013.jpg)
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP1-80959] - Staining of human pancreas shows moderate nuclear postivity in islets of Langerhans.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-Paraffin-NBP1-80959-img0014.jpg)
![Western Blot: PSMC1 Antibody [NBP1-80959] - Characterization of potent & selective NMT inhibitors.(a) Structure & dual NMT1/NMT2 inhibitory potency of compound 1. (b) Crystal structure of 1 (grey) bound to NMT1 in the presence of Myr-CoA showing key water molecules (red spheres) & polar interactions (dashed lines). Key residues (NMT1 numbering) are shown for NMT1 (blue, PDB 4C2Y) & NMT2 (pink, PDB 4C2X), & for NMT1 with 1 bound (green, PDB 4C2Z). Myr-CoA or the Myr-CoA analogue (NHM) is shown in black/grey. Image generated in PyMOL (0.99rc6, DeLano Scientific LLC, //pymol.sourceforge.net/). (c) Viability (MTS assay) of HeLa cells exposed to compound 1 at concentrations & time points indicated; error bars, s.d. (n=6). (d) Compound 1 inhibits NMT activity dose-dependently in HeLa cells (BPD=before pull-down; PD=after pull-down on streptavidin-coated beads). In-gel fluorescence after YnMyr tagging was quantified (ImageJ) & the response (% relative intensity; error bars, s.d. (n=2)) plotted using GraFit 7.0 to determine TC50 (see Fig. 2a for TC50 determined for compound 1). Western blots against protein substrates of NMT show dose-dependent reduction in enrichment following inhibition (tubulin: non-substrate loading control). (e) Cells treated with azidohomoalanine (AHA), cycloheximide (CHX) or inhibitor 1 (or DMSO vehicle), were lysed & ligated (CuAAC) to an alkyne-TAMRA reagent67. In-gel fluorescence demonstrates inhibition of protein synthesis by CHX, but not by 1. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25255805), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-80959_rabbit-polyclonal-psmc1-antibody-3102024161608.jpg)
![Western Blot: PSMC1 Antibody [NBP1-91476] - Lane 1: 10 ug proteasome fraction from C57B1/6J mouse brain, lane 2: 10 ug proteasome fraction from BLAB/C mouse brain. Primary PSMC1 antibody at 1:500. Secondary antibody: Anti-rabbit HRP antibody at 1:5000.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Western-Blot-NBP1-91476-img0003.jpg "Western Blot: PSMC1 Antibody [NBP1-91476] - Lane 1: 10 ug proteasome fraction from C57B1/6J mouse brain, lane 2: 10 ug proteasome fraction from BLAB/C mouse brain. Primary PSMC1 antibody at 1:500. Secondary antibody: Anti-rabbit HRP antibody at 1:5000.")
![Western Blot: PSMC1 Antibody [NBP1-91476] - Mouse Brain lysate, PSMC1 antibody a 1 ug/mL.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Western-Blot-NBP1-91476-img0002.jpg)
![Simple Western: PSMC1 Antibody [NBP1-91476] - Mouse whole spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this PSMC1 antibody. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Simple-Western-NBP1-91476-img0004.jpg)
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-NBP2-14857-img0010.jpg "Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP2-14857] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PSMC1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-NBP2-14857-img0009.jpg)
![Immunoprecipitation: PSMC1 Antibody [NBP2-14857] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC1 was successfully immunoprecipitated by rabbit anti-PSMC1 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunoprecipitation-NBP2-14857-img0001.jpg)
![Western Blot: PSMC1 Antibody [NBP3-17916] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/PSMC1-Antibody-Western-Blot-NBP3-17916-img0002.jpg "Western Blot: PSMC1 Antibody [NBP3-17916] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunohistochemistry-Paraffin: PSMC1 Antibody [NBP3-17916] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunohistochemistry-Paraffin-NBP3-17916-img0001.jpg)
![Western Blot: PSMC1 Antibody [NBP3-35591] - Western blot analysis of various lysates using PSMC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-35591_rabbit-psmc1-pab-10122024120696.jpg "Western Blot: PSMC1 Antibody [NBP3-35591] - Western blot analysis of various lysates using PSMC1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.")
![Western Blot: PSMC1 Antibody [NBP2-14856] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC1 was successfully immunoprecipitated by rabbit anti-PSMC1 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).](http://images.novusbio.com/fullsize/PSMC1-Antibody-Immunoprecipitation-NBP2-14856-img0001.jpg "Western Blot: PSMC1 Antibody [NBP2-14856] - Samples: Whole cell lysate from 293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PSMC1 antibody used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. PSMC1 was successfully immunoprecipitated by rabbit anti-PSMC1 antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).")
![Western Blot: Proteasome 19S S4 Antibody [NB120-3317] - Analysis of proteasome 19S subunit S4 on HeLa cell lysate.](http://images.novusbio.com/fullsize/Proteasome-19S-S4-Antibody-Western-Blot-NB120-3317-img0003.jpg "Western Blot: Proteasome 19S S4 Antibody [NB120-3317] - Analysis of proteasome 19S subunit S4 on HeLa cell lysate.")
![Western Blot: Proteasome 19S S4 Overexpression Lysate (Adult Normal) [NBL1-14886] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proteasome 19S S4.](http://images.novusbio.com/fullsize/Proteasome-19S-S4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14886-img0002.jpg "Western Blot: Proteasome 19S S4 Overexpression Lysate (Adult Normal) [NBL1-14886] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Proteasome 19S S4.")