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![Western Blot: iASPP Antibody [NBP2-48732] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/iASPP-Antibody-Western-Blot-NBP2-48732-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: iASPP Antibody [NBP2-48732] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: iASPP Antibody [NBP2-48732] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48732-img0004.jpg)
![Immunohistochemistry-Paraffin: iASPP Antibody [NBP2-48732] - Staining of human skin shows strong cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunohistochemistry-Paraffin-NBP2-48732-img0003.jpg)
![Western Blot: iASPP Antibody [NB100-88155] - Detection of Human and Mouse iASPP by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), HeLa (50, 15 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody NB100-88155 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/iASPP-Antibody-Western-Blot-NB100-88155-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: iASPP Antibody [NB100-88155] - Detection of Human and Mouse iASPP by Western Blot. Samples: Whole cell lysate from 293T (50, 15, 5 ug), HeLa (50, 15 ug), and mouse NIH3T3 (50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-iASPP antibody NB100-88155 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: iASPP Antibody [NB100-88155] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiiASPP antibody used for IP at 6 ug per reaction. iASPP was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated iASPP, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunoprecipitation-NB100-88155-img0004.jpg)
![Western Blot: iASPP Antibody [NBP1-77912] - Shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers.](http://images.novusbio.com/fullsize/iASPP-Antibody-Western-Blot-NBP1-77912-img0004.jpg "Western Blot: iASPP Antibody [NBP1-77912] - Shows detection of a band at ~100 kDa (arrowhead) corresponding to isoform 1 of iASPP in MCF7 whole cell lysates. Preincubation with immunizing peptide blocks specific band staining (data not shown). Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed with the primary antibody diluted to 1:1,500 in 5% BLOTTO/PBS overnight at 4C. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers.")
![Immunohistochemistry: iASPP Antibody [NBP1-77912] - Staining of myocytes in human heart tissue. Tissue was formalin-fixed and paraffin embedded. Brown color indicates presence of protein, blue color shows cell nuclei.](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunohistochemistry-NBP1-77912-img0005.jpg)
![Western Blot: iASPP Antibody [H00010848-B01P] - Analysis of PPP1R13L expression in transfected 293T cell line by PPP1R13L polyclonal antibody. Lane1:PPP1R13L transfected lysate(91.08 KDa). Lane2:Non-transfected lysate.](http://images.novusbio.com/fullsize/iASPP-Antibody-Western-Blot-H00010848-B01P-img0004.jpg "Western Blot: iASPP Antibody [H00010848-B01P] - Analysis of PPP1R13L expression in transfected 293T cell line by PPP1R13L polyclonal antibody. Lane1:PPP1R13L transfected lysate(91.08 KDa). Lane2:Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: iASPP Antibody [H00010848-B01P] - Analysis of purified antibody to PPP1R13L on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunocytochemistry-Immunofluorescence-H00010848-B01P-img0003.jpg)
![Immunohistochemistry-Paraffin: iASPP Antibody [NBP2-38879] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.](http://images.novusbio.com/fullsize/iASPP-Antibody-Immunohistochemistry-Paraffin-NBP2-38879-img0002.jpg "Immunohistochemistry-Paraffin: iASPP Antibody [NBP2-38879] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.")
![Western Blot: iASPP Antibody [H00010848-D01P] - Analysis of PPP1R13L expression in transfected 293T cell line by PPP1R13L polyclonal antibody.Lane 1: PPP1R13L transfected lysate(89.10 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/iASPP-Antibody-Western-Blot-H00010848-D01P-img0002.jpg "Western Blot: iASPP Antibody [H00010848-D01P] - Analysis of PPP1R13L expression in transfected 293T cell line by PPP1R13L polyclonal antibody.Lane 1: PPP1R13L transfected lysate(89.10 KDa).Lane 2: Non-transfected lysate.")