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![Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in human cell line BEWO.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-13754-img0012.jpg "Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in human cell line BEWO.")
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody [NBP2-13754] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13754-img0007.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0010.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP2-13754] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-13754-img0011.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0008.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0009.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP2-13754] - Staining of human tonsil shows strong granular cytoplasmic positivity in non-germinal and germinal center cells.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-13754-img0013.jpg)
![Western Blot: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Western blot analysis of extracts of various cell lines, using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Western-Blot-NBP3-16766-img0004.jpg "Western Blot: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Western blot analysis of extracts of various cell lines, using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunofluorescence analysis of U-2 OS cells using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunocytochemistry-Immunofluorescence-NBP3-16766-img0003.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunohistochemistry of paraffin-embedded human colon carcinoma using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunohistochemistry-Paraffin-NBP3-16766-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunofluorescence analysis of NIH-3T3 cells using Prohibitin 2 (Prohibitin 2) Rabbit mAb (NBP3-16766) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunocytochemistry-Immunofluorescence-NBP3-16766-img0002.jpg)
![Immunoprecipitation: Prohibitin 2 Antibody (8I10B1) [NBP3-16766] - Immunoprecipitation analysis of 300ug extracts of SKOV3 cells using 3ug Prohibitin 2 antibody (NBP3-16766). Western blot was performed from the immunoprecipitate using Prohibitin 2 antibody (NBP3-16766) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-8I10B1-Immunoprecipitation-NBP3-16766-img0005.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-33463-img0007.jpg "Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (30 ug of whole cell lysate) A: H1299 B: Hela 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: Prohibitin 2 Antibody [NBP1-33463] - Analysis of paraformaldehyde-fixed A431, using REA (NBP1-33463) antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33463-img0004.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Paraffin-embedded mouse urinary bladder . Prohibitin 2 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33463-img0008.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP1-33463] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-33463-img0006.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NBP1-33463] - Paraffin-embedded U87 xenograft; antibody dilution 1:500.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-33463-img0005.jpg)
![Western Blot: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Western blot analysis of Prohibitin 2 on different lysates with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution. Lane 1: Huh7 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: PANC-1 cell lysate (15 ug/Lane) Lane 5: RAW264.7 cell lysate (15 ug/Lane) Lane 6: NIH/3T3 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (15 ug/Lane) Lane 8: Mouse liver tissue lysate (30 ug/Lane) Lane 9: Rat liver tissue lysate (30 ug/Lane) Lane 10: Mouse heart tissue lysate (30 ug/Lane) Lane 11: Rat heart tissue lysate (30 ug/Lane) Lane 12: Mouse testis tissue lysate (30 ug/Lane) Lane 13: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32900) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-288202412461642.jpg "Western Blot: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Western blot analysis of Prohibitin 2 on different lysates with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution. Lane 1: Huh7 cell lysate (15 ug/Lane) Lane 2: HeLa cell lysate (15 ug/Lane) Lane 3: HepG2 cell lysate (15 ug/Lane) Lane 4: PANC-1 cell lysate (15 ug/Lane) Lane 5: RAW264.7 cell lysate (15 ug/Lane) Lane 6: NIH/3T3 cell lysate (15 ug/Lane) Lane 7: PC-12 cell lysate (15 ug/Lane) Lane 8: Mouse liver tissue lysate (30 ug/Lane) Lane 9: Rat liver tissue lysate (30 ug/Lane) Lane 10: Mouse heart tissue lysate (30 ug/Lane) Lane 11: Rat heart tissue lysate (30 ug/Lane) Lane 12: Mouse testis tissue lysate (30 ug/Lane) Lane 13: Rat testis tissue lysate (30 ug/Lane) Predicted band size: 33 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32900) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32900) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-298202414262693.jpg)
![Immunocytochemistry/ Immunofluorescence: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Immunocytochemistry analysis of HeLa cells labeling Prohibitin 2 with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Prohibitin 2 antibody (NBP3-32900) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-2920241192365.jpg)
![Flow Cytometry: Prohibitin 2 Antibody (JE35-46) [NBP3-32900] - Flow cytometric analysis of HeLa cells labeling Prohibitin 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32900, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32900_rabbit-prohibitin-2-mab-je35-46-29202416453249.jpg)
![Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunohistochemistry-NB100-1807-img0004.jpg "Immunohistochemistry-Paraffin: Prohibitin 2 Antibody [NB100-1807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- REA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Prohibitin 2 Antibody [NB100-1807] - Detection of Human REA by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-REA antibodies NB100-1807, another REA Ab, NB100-1808, and NB100-1809 each used for IP at 3 mcg/mg lysate. Immunoprecipitated REA was blotted using BL1706 (upper blot) or BL1707 (lower blot). Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunoprecipitation-NB100-1807-img0001.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP2-50549] - 30 ug mouse Neuro2A whole cell lysate separated by 4-12% PAGE, blotted on PVDF membranes and immunodetected with PHB2 antibody NBP2-50549 at 1:1000 in 5% (w/v) dry milk powder in TBST. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-50549-img0001.jpg "Western Blot: Prohibitin 2 Antibody [NBP2-50549] - 30 ug mouse Neuro2A whole cell lysate separated by 4-12% PAGE, blotted on PVDF membranes and immunodetected with PHB2 antibody NBP2-50549 at 1:1000 in 5% (w/v) dry milk powder in TBST. WB image submitted by a verified customer review.")
![Western Blot: Prohibitin 2 Antibody [NBP2-50550] - Analysis using a PHB2 polyclonal antibody in MDA-MB435 cell lysates (35 ug per lane).](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-50550-img0004.jpg "Western Blot: Prohibitin 2 Antibody [NBP2-50550] - Analysis using a PHB2 polyclonal antibody in MDA-MB435 cell lysates (35 ug per lane).")
![Western Blot: Prohibitin 2 Antibody [NBP2-50550] - Analysis of PHB2 (arrow) using a PHB2 polyclonal antibody in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2).](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-50550-img0001.jpg)
![Western Blot: Prohibitin 2 Antibody [NBP2-50550] - Analysis using a PHB2 polyclonal antibody in mouse kidney tissue lysates (35 ug per lane).](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP2-50550-img0003.jpg)
![Western Blot: Prohibitin 2 Antibody [NB100-1809] - Detection of Human and Mouse REA on HeLa whole cell lysate using NB100-1809. REA was also immunoprecipitated using rabbit anti-REA antibodies NB100-1807 and NB100-1808.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NB100-1809-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Prohibitin 2 Antibody [NB100-1809] - Detection of Human and Mouse REA on HeLa whole cell lysate using NB100-1809. REA was also immunoprecipitated using rabbit anti-REA antibodies NB100-1807 and NB100-1808.")
![Western Blot: Prohibitin 2 Antibody (SR1516) [NBP3-22147] - Western blot analysis using NBP3-22147 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22147_rabbit-prohibitin-2-mab-sr1516-672023106283.jpg "Western Blot: Prohibitin 2 Antibody (SR1516) [NBP3-22147] - Western blot analysis using NBP3-22147 in HeLa cell lysate.")
![Western Blot: Prohibitin 2 Antibody [NBP1-98312] - WB Suggested Anti-Phb2 Antibody. Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience.](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-98312-img0004.jpg "Western Blot: Prohibitin 2 Antibody [NBP1-98312] - WB Suggested Anti-Phb2 Antibody. Positive Control: Lane 1: 80ug mouse brain extractLane 2: 80ug rat brain extract. Primary Antibody Dilution : 1:500. Secondary Antibody : IRDye 800 CW goat anti-rabbit from Li-COR Bioscience.")
![Western Blot: Prohibitin 2 Antibody [NBP1-98312] - Mouse Heart Lysate 1.0ug/ml, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Western-Blot-NBP1-98312-img0003.jpg)
![Immunoprecipitation: Prohibitin 2 Antibody [NBP1-98312] - NT2 CELL/BRAIN TISSUE](http://images.novusbio.com/fullsize/Prohibitin-2-Antibody-Immunoprecipitation-NBP1-98312-img0002.jpg)
![Western Blot: Prohibitin 2 Overexpression Lysate (Adult Normal) [NBP2-11355] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Prohibitin 2.](http://images.novusbio.com/fullsize/Prohibitin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11355-img0001.jpg "Western Blot: Prohibitin 2 Overexpression Lysate (Adult Normal) [NBP2-11355] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Prohibitin 2.")