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![Western Blot: ADE2 Antibody [NBP1-90235] - Analysis using Anti-PAICS antibody NBP1-90235 (A) shows similar pattern to independent antibody NBP2-58966 (B).](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP1-90235-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ADE2 Antibody [NBP1-90235] - Analysis using Anti-PAICS antibody NBP1-90235 (A) shows similar pattern to independent antibody NBP2-58966 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Analysis in human liver and skeletal muscle tissues using NBP1-90235 antibody. Corresponding PAICS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0015.jpg)
![Western Blot: ADE2 Antibody [NBP1-90235] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP1-90235-img0008.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0010.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0011.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0012.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0013.jpg)
![Simple Western: ADE2 Antibody [NBP1-90235] - Simple Western lane view shows a specific band for ADE2 in 0.2 mg/ml of RT-4 and U-251MG lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ADE2-Antibody-Simple-Western-NBP1-90235-img0005.jpg)
![Simple Western: ADE2 Antibody [NBP1-90235] - Electropherogram image(s) of corresponding Simple Western lane view. ADE2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).](http://images.novusbio.com/fullsize/ADE2-Antibody-Simple-Western-NBP1-90235-img0006.jpg)
![Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0005.jpg "Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - Blot showing the effect of hypoxia on the protein expression levels of the purine biosynthetic enzymes. HIF-1 alpha is stabilized in hypoxia as expected, and no significant increase in the purine enzymes was detected between normoxic (21% oxygen) and hypoxic (1% oxygen) growth conditions. The positions of molecular markers surrounding each band of interest are shown for each blot. ADSL (NBP2-03107), ATIC (NBP2-01941), FGAMS (NBP1-84691), GART (H00002618-M01), HIF-1a (NB100-449), PAICS (NBP2-02817), PPAT (NBP2-02056). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32439803/) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: ADE2 Antibody (OTI1B4) [NBP2-02817] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ADE2.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Immunocytochemistry-Immunofluorescence-NBP2-02817-img0004.jpg)
![Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ADE2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Flow-Cytometry-NBP2-02817-img0002.jpg)
![Western Blot: ADE2 Antibody (OTI1B4) [NBP2-02817] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADE2.](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Western-Blot-NBP2-02817-img0003.jpg)
![Flow Cytometry: ADE2 Antibody (OTI1B4) [NBP2-02817] - Analysis of Jurkat cells, using anti-ADE2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/ADE2-Antibody-OTI1B4-Flow-Cytometry-NBP2-02817-img0001.jpg)
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15290] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP2-15290-img0002.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15290] - Paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15290] - Paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP2-15290-img0001.jpg)
![Western Blot: ADE2 Antibody [NBP2-15290] -Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAICS antibody [N1C1] diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-15290_rabbit-polyclonal-ade2-antibody-western-blot-811202494659..png)
![Western Blot: ADE2 Antibody [NBP2-58966] - Analysis using Anti-PAICS antibody NBP2-58966 (A) shows similar pattern to independent antibody NBP1-90235 (B).](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP2-58966-img0003.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ADE2 Antibody [NBP2-58966] - Analysis using Anti-PAICS antibody NBP2-58966 (A) shows similar pattern to independent antibody NBP1-90235 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: ADE2 Antibody [NBP2-58966] - Staining of human cell line HEK 293 shows localization to cytosol.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58966-img0001.jpg)
![Western Blot: ADE2 Antibody [NBP2-15289] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ADE2-Antibody-Western-Blot-NBP2-15289-img0003.jpg "Western Blot: ADE2 Antibody [NBP2-15289] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP2-15289] - U87 xenograft, using PAICS antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP2-15289-img0001.jpg)
![Western Blot: ADE2 Antibody (4F4) [H00010606-M01] - PAICS monoclonal antibody (M01), clone 4F4. Analysis of PAICS expression in human placenta.](http://images.novusbio.com/fullsize/ADE2-Antibody-4F4-Western-Blot-H00010606-M01-img0002.jpg "Western Blot: ADE2 Antibody (4F4) [H00010606-M01] - PAICS monoclonal antibody (M01), clone 4F4. Analysis of PAICS expression in human placenta.")
![Sandwich ELISA: ADE2 Antibody (4F4) [H00010606-M01] - Detection limit for recombinant GST tagged PAICS is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ADE2-Antibody-4F4-Sandwich-ELISA-H00010606-M01-img0001.jpg)
![Western Blot: ADE2 Overexpression Lysate (Adult Normal) [NBL1-14073] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADE2.](http://images.novusbio.com/fullsize/ADE2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14073-img0002.jpg "Western Blot: ADE2 Overexpression Lysate (Adult Normal) [NBL1-14073] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADE2.")
![Western Blot: ADE2 Overexpression Lysate (Adult Normal) [NBP2-11447] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADE2.](http://images.novusbio.com/fullsize/ADE2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11447-img0001.jpg "Western Blot: ADE2 Overexpression Lysate (Adult Normal) [NBP2-11447] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ADE2.")
![SDS-Page: ADE2 Recombinant Protein [NBP2-51641] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/ADE2-Recombinant-Protein-SDS-Page-NBP2-51641-img0001.jpg "SDS-Page: ADE2 Recombinant Protein [NBP2-51641] - 15% SDS Page (3 ug)")