| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1979-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37597-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37596-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14028 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14028 | |||
![Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Western-Blot-NBP2-37597-img0003.jpg "Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - ARPE-19 monolayer on glass substrate. Fixative 4% PFA Blocking Goat Serum Dilution 1:200. Incubation 1h @RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunocytochemistry-NBP2-37597-img0008.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunohistochemistry-Paraffin-NBP2-37597-img0007.jpg)
![Flow Cytometry: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Flow cytometric analysis of HepG2 cells using OTX2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Flow-Cytometry-NBP2-37597-img0005.jpg)
![Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Western-Blot-NBP2-37597-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Immunofluorescence analysis of HepG2 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunocytochemistry-Immunofluorescence-NBP2-37597-img0004.jpg)
![ELISA: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-ELISA-NBP2-37597-img0001.jpg)
![Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Western-Blot-NBP2-37596-img0003.jpg "Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of U251 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Immunocytochemistry-Immunofluorescence-NBP2-37596-img0004.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of colon tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Immunohistochemistry-Paraffin-NBP2-37596-img0009.jpg)
![Western Blot: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Western-Blot-NBP2-37596-img0002.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Analysis of stomach tissues (left) and brain tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-Immunohistochemistry-Paraffin-NBP2-37596-img0008.jpg)
![ELISA: Otx2 Antibody (1H12G8B2) [NBP2-37596] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12G8B2-ELISA-NBP2-37596-img0001.jpg)
![Western Blot: Otx2 Antibody (PSH01-61) [NBP3-32683] - Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/1,000 dilution. Lane 1: 293T transfected with FLAG-tagged empty control cell lysate Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32683) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-278202415212415.jpg "Western Blot: Otx2 Antibody (PSH01-61) [NBP3-32683] - Western blot analysis of Otx2 on different lysates with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/1,000 dilution. Lane 1: 293T transfected with FLAG-tagged empty control cell lysate Lane 2: 293T transfected with FLAG-tagged Otx2 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32683) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Otx2 Antibody (PSH01-61) [NBP3-32683] - Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32683) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-29820241430410.jpg)
![Immunocytochemistry/ Immunofluorescence: Otx2 Antibody (PSH01-61) [NBP3-32683] - Immunocytochemistry analysis of 293T overexpress with or without Otx2 cells labeling Otx2 with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/10,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Otx2 antibody (NBP3-32683) at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-29202410484846.jpg)
![Flow Cytometry: Otx2 Antibody (PSH01-61) [NBP3-32683] - Flow cytometric analysis of 293T overexpress with or without Otx2 cells labeling Otx2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32683, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32683_rabbit-otx2-mab-psh01-61-392024884675.jpg)
![Western Blot: Otx2 Antibody [NBP3-13176] - Whole cell extract (37.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otx2 antibody (NBP3-13176) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Otx2-Antibody-Western-Blot-NBP3-13176-img0001.jpg "Western Blot: Otx2 Antibody [NBP3-13176] - Whole cell extract (37.5 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Otx2 antibody (NBP3-13176) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.")
![Immunohistochemistry-Paraffin: Otx2 Antibody [NBP3-13176] - Otx2 antibody detects Otx2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: Otx2 stained by Otx2 antibody (NBP3-13176) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Otx2-Antibody-Immunohistochemistry-Paraffin-NBP3-13176-img0003.jpg)
![Immunohistochemistry-Frozen: Otx2 Antibody [NBP3-13176] - Otx2 antibody detects Otx2 protein by immunohistochemical analysis. Sample: Frozen-sectioned rat E13.5 brain. Green: Otx2 stained by Otx2 antibody (NBP3-13176) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Otx2-Antibody-Immunohistochemistry-Frozen-NBP3-13176-img0002.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/Otx2-Antibody-Immunohistochemistry-Paraffin-NBP1-86533-img0004.jpg "Immunohistochemistry-Paraffin: Otx2 Antibody [NBP1-86533] - Staining of human hippocampus shows moderate nuclear positivity in neuronal cells.")
![Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384] - Western blot analysis of extracts of various cell lines, using Otx2 Rabbit mAb (NBP3-16384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Otx2-Antibody-6I3J0-Western-Blot-NBP3-16384-img0001.jpg "Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384] - Western blot analysis of extracts of various cell lines, using Otx2 Rabbit mAb (NBP3-16384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: Otx2 Antibody (6I3J0) [NBP3-16384] - Western blot analysis of extracts of various cell lines, using Otx2 Rabbit mAb (NBP3-16384) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Otx2-Antibody-6I3J0-Western-Blot-NBP3-16384-img0002.jpg)
![Western Blot: Otx2 Overexpression Lysate (Adult Normal) [NBL1-14015] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Otx2.](http://images.novusbio.com/fullsize/Otx2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14015-img0002.jpg "Western Blot: Otx2 Overexpression Lysate (Adult Normal) [NBL1-14015] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Otx2.")
![Western Blot: Otx2 Antibody (SR1525) [NBP3-22054] - Western blot analysis using NBP3-22054 in Y79 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22054_rabbit-otx2-mab-sr1525-57202317215131.jpg "Western Blot: Otx2 Antibody (SR1525) [NBP3-22054] - Western blot analysis using NBP3-22054 in Y79 cell lysate.")