Hu,
Mu,
Rt,
Po,
Av,
Bv,
Ca,
Ch,
ChHa,
Dr,
Eq,
Fe,
Ha,
Ma-Op,
Pm,
Rb,
Sh,
ZeSpecies Glossary
Reactivity: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
WB, Simple Western, ICC/IF, IHC, IP, ChIP, KD, Single-Cell Western
Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP, KD, Single-Cell Western
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
![Analysis of cell line lysates probed with mouse mAb to GAPDH (theoretical molecular weight: 36 kDa), NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.](http://images.novusbio.com/fullsize/GAPDH-Antibody-1D4-Western-Blot-NB300-221-img0008.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Notch1, ApoER2 & Dab1 colocalize in hippocampal neurons. (A) Representative image of fluorescent immunolabeling shows that Notch1 is localized in a subset of CA3 neurons, co-expressing Dab1 & ApoER2 (magnified insert). ApoER2 is highly expressed in all CA3 neurons. (B) Fluorescent immunolabeling shows the distribution of Notch1 & Dab1 as compared to Arc/Arg3.1 in CA3 neurons. (B′) Close up of a dendrite showing colocalization of the three proteins (white arrowheads). (C) Western blot analysis on immunoprecipitated samples from whole hippocampal lysate shows that Notch1 displays a strong interaction with Dab1 whereas Notch1 & ApoER2 interaction appears weaker in the preparation (n = 4 independent experiments). (D) Immunolabeling on immunoprecipitated samples from whole cortical lysates show that Notch1 & Dab1 interact. ApoER2 is bound to both Notch1 & Dab1 (n = 3 independent experiments). (E) Co-IP from synaptosomal membrane fractions reveals a stronger interaction between Notch1 & ApoER2, which is reduced in the N1cKO (n = 3 independent pulldowns). In (C–E) GAPDH is used as a loading control for the inputs & to detect contamination in the IP samples. Scale bar in (A) is 50 μm & (B) 25 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26635527), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416205131.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Reduced expression of UNC5A in FancC−/− cells. a Reduced expression of Unc5A in brain cortex of FancC−/− mice compared to wild-type littermates. Western blotting was performed with the indicated antibodies. Each lane represents a different animal (WT: n = 6; FancC−/−: n = 8). b Bar graph represents ratio of Unc5A normalized to tubulin from WT (n = 6) & FancC−/− mice (n = 8). c Bar graph representation of UNC5A expression normalized to Sdha & Tbp from wild-type (WT) & FancC−/−-derived fibroblasts (n = 3). RLU: Relative light units. d–f HEK293T cells were transfected with UNC5AICD, FANCE & FANCC constructs expressing full-length FANCC (in d), FANCC1–306 (in e) or FANCC307–558 (in f). Constructs were transfected at a molecular ratio of 1:1:1 or with 10 times the molar amount of FANCE, FANCC or UNC5AICD as indicated in the figure. The total amount of transfected plasmid was equalized for all strategies with control empty vectors. Representative immunoblots performed with anti-HA (UNC5AICD), anti-FANCC, anti-FANCE or anti-GAPDH antibodies are shown. Arrows indicate appropriate protein bands. Bar graphs represent the mean fold change ± SEM of UNC5A protein levels normalized to GAPDH compared to 1:1:1 transfection controls in at least 4 separate experiments. *p < 0.05; **p < 0.005 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30213274), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416203715.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Depletion of RAD21 & other cohesin subunits leads to differentiation of ESCs.(A): qPCR analysis of esiRNA knock-down efficiency for indicated genes (48 h RNAi, n = 3, error bars denote s.d. & *, ** indicate p<0.05 & 0.01 respectively). Transfection of a non-targeting esiRNA (Luc) was used as a control. Numbers adjacent to the gene names indicate independent, non-overlapping esiRNAs transfected. (B): Western Blot analysis of esiRNA knock-down efficiency for indicated genes (48 h RNAi; esi-1 & esi-2 indicate independent, non-overlapping esiRNAs transfected). Transfection of a non-targeting esiRNA (Luc) was used as a control. GAPDH served as a protein loading control. (C): Alkaline phosphatase staining of ESCs, which had been transfected with esiRNAs targeting RAD21, SMC1a & SMC3 (72 h post RNAi) showed a strong differentiation phenotype compared to a control (Luc). Nanog depletion served as a positive control for ESC differentiation. Scale bars correspond to 100 µm. (D): Quantification of alkaline phosphatase staining (n = 3) separated into undifferentiated (green), mixed (yellow) & differentiated colonies (red). (E+F): qPCR validation of expression changes of (E) stem cell maintenance genes & (F) lineage marker genes upon knock-down of RAD21 (two independent esiRNAs) & Luc control (48 h RNAi, n = 3, error bars denote s.d. & *, **, *** indicate p<0.05, 0.01 & 0.001 respectively). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/21589869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416205163.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Application of the approach to other cell lines. (A) HT1080 cells (2 × 105) & HeLa cells (1 × 105) were plated in the wells of a 12 well plate & the digitonin concentration required for optimal extraction of the cytosol was determined as in Figure1. (B) The remainder of the protocol from Figure 2 was then applied using 100 μg/ml digitonin as the optimal cytosolic extract in each case. An aliquot of each extract was then analyzed by 4-12% SDS PAGE followed by Western blotting & probing with antibodies to GAPDH, BiP & Histone H3 (Used as an alternative nuclear marker due to weakness of the Lamin A signal in HeLa cells). (C) Samples were analyzed by 4-12% SDS PAGE followed by staining with Coomassie blue & then silver staining showing the difference in protein composition of each sample. The bracket alongside the gels marks the position of Histones in the RIPA nuclear extract & the asterisk next to the silver stained gels denotes protein bands unique to the E-RIPA extracts. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/20003239), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-3102024162187.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Role of the TRIM11-USP14 axis in cell growth & apoptosis. a Levels of endogenous TRIM11 in HCT116 cells grown at 43 °C for 0 or 90 min, or at 43 °C for 90 min, & then at 37 °C for 60 or 120 min. b, c Levels of endogenous TRIM11 (b), or Flag-TRIM11, the indicated proteasome subunits, & USP14 (c) in the SN & P fractions of HCT116 cells with & without heat shock at 43 °C for 90 min. d, g TRIM11 overexpression (d) or knockdown (g) HCT116 cells & the corresponding control cells grown under unstressed, heat shock, & heat shock plus recovery conditions were analyzed for K48 polyUb conjugates & caspase-3 activation. e, f, h Apoptosis in TRIM11 overexpression (e, f) or knockdown (h) HCT116 cells, & the corresponding control HCT116 cells, treated with & without heat shock. i, j Caspase-3 activation (i) & apoptosis (j) in HCT116 cells expressing the indicated proteins and grown under normal or heat stress conditions. k–m K48 polyUb conjugates, caspase-3 activation (k), amyloid-like fibrils (l), & apoptosis (m) in HCT116 cells expressing the indicated proteins and grown under normal & heat stress conditions. In e, f, h, j, l, & m, data represent mean ± SEM (n = 3 unless otherwise indicated). *P < 0.05; **P < 0.01; ***P < 0.001. Uncropped blots are presented in Supplementary Fig. 14 Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-018-03499-z), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-221_mouse-monoclonal-gapdh-antibody-1d4-imgenex-img-5019a-310202416212324.jpg)
![Western Blot: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of FGFR1OP expression in transfected 293T cell line by FGFR1OP monoclonal antibody (M01), clone 2B1. Lane 1: FGFR1OP transfected lysate(43.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-2B1-Western-Blot-H00011116-M01-img0006.jpg "Western Blot: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of FGFR1OP expression in transfected 293T cell line by FGFR1OP monoclonal antibody (M01), clone 2B1. Lane 1: FGFR1OP transfected lysate(43.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: FGFR1OP Antibody (2B1) [H00011116-M01] - Analysis of monoclonal antibody to FGFR1OP on HeLa cell. Antibody concentration 10 ug/mL.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-2B1-Immunocytochemistry-Immunofluorescence-H00011116-M01-img0004.jpg)
![Immunohistochemistry-Frozen: FGFR1OP Antibody (2B1) [H00011116-M01] - Rabbit frozen 4% paraformalehyde fixed brain sections. Double labeling with FGFR1OP (Basal bodies in green) and GT335 (cilia in red) antibodies of ependymal whole mount from brain of rabbit kit. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-2B1-Immunohistochemistry-Frozen-H00011116-M01-img0009.jpg)
![ELISA: FGFR1OP Antibody (2B1) [H00011116-M01] - Detection limit for recombinant GST tagged FGFR1OP is approximately 0.03 ng/mL as a capture antibody.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-2B1-ELISA-H00011116-M01-img0007.jpg)
![Western Blot: FGFR1OP2 Antibody [NBP2-16479] - Sample (50 ug of whole cell lysate) A: mouse stomach, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Western-Blot-NBP2-16479-img0002.jpg "Western Blot: FGFR1OP2 Antibody [NBP2-16479] - Sample (50 ug of whole cell lysate) A: mouse stomach, 12% SDS PAGE gel, diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: FGFR1OP2 Antibody [NBP2-16479] - Immunofluorescence analysis of methanol-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16479-img0004.jpg)
![Immunohistochemistry-Paraffin: FGFR1OP2 Antibody [NBP2-16479] - Immunohistochemical analysis of paraffin-embedded H441 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Immunohistochemistry-Paraffin-NBP2-16479-img0003.jpg)
![Western Blot: FGFR1OP2 Antibody [NBP2-16479] - Sample (30 ug of whole cell lysate) A: MCF-7 12% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Western-Blot-NBP2-16479-img0001.jpg)
![Western Blot: FGFR1OP2 Antibody [NBP1-84148] - Analysis in control (vector only transfected HEK293T lysate) and FGFR1OP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Western-Blot-NBP1-84148-img0009.jpg "Western Blot: FGFR1OP2 Antibody [NBP1-84148] - Analysis in control (vector only transfected HEK293T lysate) and FGFR1OP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: FGFR1OP2 Antibody [NBP1-84148] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84148-img0008.jpg)
![Immunohistochemistry-Paraffin: FGFR1OP2 Antibody [NBP1-84148] - Staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84148-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: FGFR1OP Antibody (2T2F9) [NBP3-33266] - Immunofluorescence analysis of HeLa cells using FGFR1OP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33266_rabbit-fgfr1op-mab-2t2f9-912202415152844.jpg "Immunocytochemistry/ Immunofluorescence: FGFR1OP Antibody (2T2F9) [NBP3-33266] - Immunofluorescence analysis of HeLa cells using FGFR1OP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: FGFR1OP Antibody (2T2F9) [NBP3-33266] - Immunofluorescence analysis of NIH/3T3 cells using FGFR1OP Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33266_rabbit-fgfr1op-mab-2t2f9-91220241418196.jpg)
![Western Blot: FGFR1OP Antibody (2T2F9) [NBP3-33266] - Western blot analysis of lysates from HeLa cells using FGFR1OP Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-33266_rabbit-fgfr1op-mab-2t2f9-91220241519552.jpg)
![Western Blot: FGFR1OP Antibody (JE64-24) [NBP3-32339] - Western blot analysis of FGFR1OP on different lysates with Rabbit anti-FGFR1OP antibody at 1/500 dilution. Lane 1: HepG2 cell lysate, 10 ug/Lane Lane 2: Mouse kidney cell lysate, 20 ug/Lane Predicted band size: 43 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody CEP43 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32339_rabbit-fgfr1op-mab-je64-24-278202416331298.jpg "Western Blot: FGFR1OP Antibody (JE64-24) [NBP3-32339] - Western blot analysis of FGFR1OP on different lysates with Rabbit anti-FGFR1OP antibody at 1/500 dilution. Lane 1: HepG2 cell lysate, 10 ug/Lane Lane 2: Mouse kidney cell lysate, 20 ug/Lane Predicted band size: 43 kDa Observed band size: 53 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody CEP43 at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: FGFR1OP Antibody (JE64-24) [NBP3-32339] - Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-FGFR1OP antibody (NBP3-32339) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody CEP43 at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32339_rabbit-fgfr1op-mab-je64-24-298202414262612.jpg)
![Western Blot: FGFR1OP2 Antibody [NBP1-84149] - Analysis in control (vector only transfected HEK293T lysate) and FGFR1OP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Western-Blot-NBP1-84149-img0006.jpg "Western Blot: FGFR1OP2 Antibody [NBP1-84149] - Analysis in control (vector only transfected HEK293T lysate) and FGFR1OP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: FGFR1OP2 Antibody [NBP1-84149] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-Immunohistochemistry-Paraffin-NBP1-84149-img0005.jpg)
![Western Blot: FGFR1OP Antibody [NBP2-16478] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-Western-Blot-NBP2-16478-img0001.jpg "Western Blot: FGFR1OP Antibody [NBP2-16478] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: FGFR1OP Antibody [NBP2-16478] - Paraffin-embedded human lung cancer. FGFR1OP antibody [N1C2] diluted at 1:500.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-Immunohistochemistry-Paraffin-NBP2-16478-img0002.jpg)
![Western Blot: FGFR1OP Antibody (SR1871) [NBP3-22568] - Western blot analysis using NBP3-22568 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22568_rabbit-fgfr1op-mab-sr1871-5720231531240.jpg "Western Blot: FGFR1OP Antibody (SR1871) [NBP3-22568] - Western blot analysis using NBP3-22568 in HeLa cell lysate.")
![Western Blot: FGFR1OP Antibody (1E8) [H00011116-M02] - Analysis of FGFR1OP expression in transfected 293T cell line by FGFR1OP monoclonal antibody (M02), clone 1E8.Lane 1: FGFR1OP transfected lysate(43.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-1E8-Western-Blot-H00011116-M02-img0002.jpg "Western Blot: FGFR1OP Antibody (1E8) [H00011116-M02] - Analysis of FGFR1OP expression in transfected 293T cell line by FGFR1OP monoclonal antibody (M02), clone 1E8.Lane 1: FGFR1OP transfected lysate(43.1 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: FGFR1OP Antibody (1E8) [H00011116-M02] - Detection limit for recombinant GST tagged FGFR1OP is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FGFR1OP-Antibody-1E8-Sandwich-ELISA-H00011116-M02-img0001.jpg)
![Western Blot: FGFR1OP2 Antibody (2G4) [H00026127-M01] - Analysis of FGFR1OP2 expression in transfected 293T cell line by FGFR1OP2 monoclonal antibody (M01), clone 2G4.Lane 1: FGFR1OP2 transfected lysate(20.175 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-2G4-Western-Blot-H00026127-M01-img0004.jpg "Western Blot: FGFR1OP2 Antibody (2G4) [H00026127-M01] - Analysis of FGFR1OP2 expression in transfected 293T cell line by FGFR1OP2 monoclonal antibody (M01), clone 2G4.Lane 1: FGFR1OP2 transfected lysate(20.175 KDa).Lane 2: Non-transfected lysate.")
![Sandwich ELISA: FGFR1OP2 Antibody (2G4) [H00026127-M01] - Detection limit for recombinant GST tagged FGFR1OP2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/FGFR1OP2-Antibody-2G4-Sandwich-ELISA-H00026127-M01-img0003.jpg)
