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![Western Blot: OLFM4 Antibody [NBP2-94723] - Analysis of extracts of various cell lines, using OLFM4 antibody (NBP2-94723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/OLFM4 Antibody-Western Blot-NBP2-94723-img0002.jpg "Western Blot: OLFM4 Antibody [NBP2-94723] - Analysis of extracts of various cell lines, using OLFM4 antibody (NBP2-94723) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: OLFM4 Antibody [NBP2-94723] - Immunofluorescence analysis of human colon carcinoma using OLFM4 Rabbit pAb (NBP2-94723) at dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/OLFM4-Antibody-Immunohistochemistry-NBP2-94723-img0001.jpg)
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-62720] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP2-62720-img0002.jpg "Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-62720] - Staining of human small intestine shows high expression.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-62720] - Analysis in human small intestine and liver tissues using Anti-OLFM4 antibody. Corresponding OLFM4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP2-62720-img0001.jpg)
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP2-62720] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP2-62720-img0003.jpg)
![Western Blot: OLFM4 Antibody (PSH01-36) [NBP3-32677] - Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (NBP3-32677) at 1/1,000 dilution. Lane 1: OLFM4 recombinant protein Lane 2: OLFM4 recombinant protein with immunising peptide Lysates/proteins at 50 ng/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32677) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32677_rabbit-olfm4-mab-psh01-36-278202416331217.jpg "Western Blot: OLFM4 Antibody (PSH01-36) [NBP3-32677] - Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (NBP3-32677) at 1/1,000 dilution. Lane 1: OLFM4 recombinant protein Lane 2: OLFM4 recombinant protein with immunising peptide Lysates/proteins at 50 ng/Lane. Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32677) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: OLFM4 Antibody (PSH01-36) [NBP3-32677] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-OLFM4 antibody (NBP3-32677) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32677) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32677_rabbit-olfm4-mab-psh01-36-298202413585582.jpg)
![Western Blot: OLFM4 Antibody (PSH01-37) [NBP3-32678] - Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (NBP3-32678) at 1/1,000 dilution. Lane 1: OLFM4 recombinant protein Lane 2: OLFM4 recombinant protein with immunising peptide Lysates/proteins at 50 ng/Lane. Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32678) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32678_rabbit-olfm4-mab-psh01-37-278202416285260.jpg "Western Blot: OLFM4 Antibody (PSH01-37) [NBP3-32678] - Western blot analysis of OLFM4 on different lysates with Rabbit anti-OLFM4 antibody (NBP3-32678) at 1/1,000 dilution. Lane 1: OLFM4 recombinant protein Lane 2: OLFM4 recombinant protein with immunising peptide Lysates/proteins at 50 ng/Lane. Exposure time: 3 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32678) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: OLFM4 Antibody (PSH01-37) [NBP3-32678] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-OLFM4 antibody (NBP3-32678) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32678) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32678_rabbit-olfm4-mab-psh01-37-29820241492675.jpg)
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP3-14432] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/OLFM4-Antibody-Immunohistochemistry-Paraffin-NBP3-14432-img0001.jpg "Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP3-14432] - Immunohistochemistry of formalin-fixed, paraffin-embedded human kidney after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: OLFM4 Antibody [NBP3-14432] - Immunohistochemistry of formalin-fixed, paraffin-embedded human heart after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/OLFM4 Antibody-Immunohistochemistry-Paraffin-NBP3-14432-img0002.jpg)
![Western Blot: OLFM4 Antibody (SR1959) [NBP3-21911] - Western blot analysis using NBP3-21911 in LnCaP cell lysate.](http://images.novusbio.com/fullsize/nbp3-21911_rabbit-olfm4-mab-sr1959-57202316582157.jpg "Western Blot: OLFM4 Antibody (SR1959) [NBP3-21911] - Western blot analysis using NBP3-21911 in LnCaP cell lysate.")
![ELISA: Human OLFM4 ELISA Kit (Colorimetric) [NBP3-18034] - Samples were spiked with high concentrations of Human OLFM4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18034_human-olfm4-elisa-kit-colorimetric-132202416232352.jpg "ELISA: Human OLFM4 ELISA Kit (Colorimetric) [NBP3-18034] - Samples were spiked with high concentrations of Human OLFM4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human OLFM4 ELISA Kit (Colorimetric) [NBP3-18034] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18034_human-olfm4-elisa-kit-colorimetric-432024983585.jpg)
![Flow Cytometry: OLFM4 Antibody (12) [NBP3-06415] - Analysis of Human OLFM4 expression in DU145 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ and stained with Purified Mouse anti-OLFM4 then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/OLFM4-Antibody-12-Flow-Cytometry-NBP3-06415-img0002.jpg "Flow Cytometry: OLFM4 Antibody (12) [NBP3-06415] - Analysis of Human OLFM4 expression in DU145 cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ and stained with Purified Mouse anti-OLFM4 then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")