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![Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Analysis in human cerebral cortex and liver tissues. Corresponding NSF RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunohistochemistry-Paraffin-NBP1-87035-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Analysis in human cerebral cortex and liver tissues. Corresponding NSF RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: NSF Antibody [NBP1-87035] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NBP1-87035-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: NSF Antibody [NBP1-87035] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87035-img0007.jpg)
![Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human testis shows moderate to strong cytoplasmic postivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunohistochemistry-Paraffin-NBP1-87035-img0016.jpg)
![Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunohistochemistry-Paraffin-NBP1-87035-img0009.jpg)
![Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunohistochemistry-Paraffin-NBP1-87035-img0014.jpg)
![Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunohistochemistry-Paraffin-NBP1-87035-img0015.jpg)
![Western Blot: NSF Antibody [NBP3-35086] - Western blot analysis of various lysates using NSF Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-35086_rabbit-nsf-pab-1012202411531080.jpg "Western Blot: NSF Antibody [NBP3-35086] - Western blot analysis of various lysates using NSF Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.")
![Immunocytochemistry/ Immunofluorescence: NSF Antibody [NBP3-35086] - Immunofluorescence analysis of BALB-3T3 cells using NSF Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35086_rabbit-nsf-pab-10122024121057.jpg)
![Immunoprecipitation: NSF Antibody [NBP3-35086] - Immunoprecipitation analysis of 600 ug extracts of Mouse brain cells using 3 ug NSF antibody. Western blot was performed from the immunoprecipitate using NSF antibody at a dilution of 1:3000.](http://images.novusbio.com/fullsize/nbp3-35086_rabbit-nsf-pab-1012202411552823.jpg)
![Immunocytochemistry/ Immunofluorescence: NSF Antibody [NBP3-35086] - Immunofluorescence analysis of U-2 OS cells using NSF Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35086_rabbit-nsf-pab-1012202411552872.jpg)
![Western Blot: NSF Antibody (9G7-3) [NBP1-19333] - Western blot analysis: Lane 1: MW marker, Lane 2: HeLa (Heat Shocked), Lane 3: Vero, Lane 4: CHO-K1, Lane 5: ESK-4.](http://images.novusbio.com/fullsize/NSF-Antibody-9G7-3-Western-Blot-NBP1-19333-img0002.jpg "Western Blot: NSF Antibody (9G7-3) [NBP1-19333] - Western blot analysis: Lane 1: MW marker, Lane 2: HeLa (Heat Shocked), Lane 3: Vero, Lane 4: CHO-K1, Lane 5: ESK-4.")
![Immunohistochemistry: NSF Antibody (9G7-3) [NBP1-19333] - Immunohistochemistry analysis of human testis tissue stained with NSF, mAb (9G7-3) at 20ug/ml.](http://images.novusbio.com/fullsize/NSF-Antibody-9G7-3-Immunohistochemistry-NBP1-19333-img0001.jpg)
![Western Blot: NSF Antibody [NBP2-19592] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody [N3C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NBP2-19592-img0003.jpg "Western Blot: NSF Antibody [NBP2-19592] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody [N3C3] diluted at 1:1000.")
![Western Blot: NSF Antibody [NBP2-19592] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NBP2-19592-img0001.jpg)
![Western Blot: NSF Antibody [NBP2-19592] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NSF antibody [N3C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NBP2-19592-img0002.jpg)
![Western Blot: NSF Antibody [NBP1-57536] - Sample Type: 1. NT-2 cells 2. mouse brain extracts Primary Antibody Dilution: 2ug/ml Secondary Antibody Dilution: 1: 20,000 Secondary Antibody: IRDye 800CW goat anti-rabbit Lane 1: 60ug/lane Lane 2: 80ug/lane](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NBP1-57536-img0004.jpg "Western Blot: NSF Antibody [NBP1-57536] - Sample Type: 1. NT-2 cells 2. mouse brain extracts Primary Antibody Dilution: 2ug/ml Secondary Antibody Dilution: 1: 20,000 Secondary Antibody: IRDye 800CW goat anti-rabbit Lane 1: 60ug/lane Lane 2: 80ug/lane")
![Western Blot: NSF Antibody [NBP1-57536] - Mouse Brain lysate, Antibody Titration: 0.2-1 ug/ml](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NBP1-57536-img0002.jpg)
![Western Blot: NSF Antibody [NB300-733]](http://images.novusbio.com/fullsize/NSF-Antibody-Western-Blot-NB300-733-img0001.jpg "Western Blot: NSF Antibody [NB300-733]")
![Immunocytochemistry/Immunofluorescence: NSF Antibody [NBP2-58843] - Staining of human cell line MCF7 shows localization to cytosol.](http://images.novusbio.com/fullsize/NSF-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58843-img0001.jpg "Immunocytochemistry/Immunofluorescence: NSF Antibody [NBP2-58843] - Staining of human cell line MCF7 shows localization to cytosol.")
![Western Blot: NSF Overexpression Lysate (Adult Normal) [NBL1-13808] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NSF.](http://images.novusbio.com/fullsize/NSF-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13808-img0002.jpg "Western Blot: NSF Overexpression Lysate (Adult Normal) [NBL1-13808] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NSF.")
![ELISA: Human NSF ELISA Kit (Colorimetric) [NBP3-39116] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39116_human-nsf-elisa-kit-colorimetric-2110202415362380.png "ELISA: Human NSF ELISA Kit (Colorimetric) [NBP3-39116] - Standard Curve Reference")
![ELISA: Human NSF - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39117] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39117_human-nsf-ready-to-use-elisa-kit-colorimetric-2110202415273897.png "ELISA: Human NSF - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39117] - Standard Curve Reference")