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![Western Blot: Neurogranin Antibody [NBP2-29349] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Western-Blot-NBP2-29349-img0001.jpg "Western Blot: Neurogranin Antibody [NBP2-29349] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-29349] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-29349-img0003.jpg)
![Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-32655] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-32655-img0013.jpg "Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-32655] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-32655] - Analysis in human cerebral cortex and pancreas tissues. Corresponding Neurogranin RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-32655-img0009.jpg)
![Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-32655] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-32655-img0010.jpg)
![Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-32655] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-32655-img0011.jpg)
![Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-32655] - Staining of human lymphoid tissues shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-32655-img0012.jpg)
![Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-278202415242663.jpg "Western Blot: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Western blot analysis of Neurogranin on different lysates with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse hippocampus tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Rat hippocampus tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 8 kDa Observed band size: 15 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32645) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32645) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-29820241420998.jpg)
![Immunohistochemistry: Neurogranin Antibody (PSH02-96) [NBP3-32645] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Neurogranin with Rabbit anti-Neurogranin antibody (NBP3-32645) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32645, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32645_rabbit-neurogranin-mab-psh02-96-298202414161816.jpg)
![SDS-Page: Recombinant Human Neurogranin Protein [H00004900-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00004900-P01-1.jpg "SDS-Page: Recombinant Human Neurogranin Protein [H00004900-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Neurogranin Antibody [NBP2-94659] - Analysis of extracts of various cell lines, using Neurogranin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Western-Blot-NBP2-94659-img0001.jpg "Western Blot: Neurogranin Antibody [NBP2-94659] - Analysis of extracts of various cell lines, using Neurogranin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: NRGN Overexpression Lysate (Adult Normal) [NBP2-09422] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRGN.](http://images.novusbio.com/fullsize/NRGN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09422-img0001.jpg "Western Blot: NRGN Overexpression Lysate (Adult Normal) [NBP2-09422] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRGN.")
![Western Blot: NRGN Overexpression Lysate (Adult Normal) [NBP2-07656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRGN.](http://images.novusbio.com/fullsize/NRGN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07656-img0001.jpg "Western Blot: NRGN Overexpression Lysate (Adult Normal) [NBP2-07656] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NRGN.")
![SDS-Page: Recombinant Human Neurogranin Protein [NBP2-51746] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Neurogranin-Recombinant-Protein-SDS-Page-NBP2-51746-img0001.jpg "SDS-Page: Recombinant Human Neurogranin Protein [NBP2-51746] - 15% SDS Page (3 ug)")
![Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-97430] - Immunochemical staining of human Neurogranin in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/Neurogranin-Antibody-Immunohistochemistry-Paraffin-NBP2-97430-img0001.jpg "Immunohistochemistry-Paraffin: Neurogranin Antibody [NBP2-97430] - Immunochemical staining of human Neurogranin in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![ELISA: Human Neurogranin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39135] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39135_human-neurogranin-ready-to-use-elisa-kit-colorimetric-2110202415302935.png "ELISA: Human Neurogranin - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39135] - Standard Curve Reference")