| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3735-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1190-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5196-SP | |||
| Carrier Free | FAB5196V-100UG | |||
| Carrier Free | FAB5196U-100UG | |||
| Carrier Free | FAB5196T-100UG | |||
| Carrier Free | FAB5196S-100UG | |||
| Carrier Free | FAB5196R-100UG | |||
| Carrier Free | FAB5196N-100UG | |||
| Carrier Free | FAB5196G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-77072 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1190-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3735-SP | |||
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![Western Blot: Notch-2 Antibody [NBP2-27347] - Analysis of NOTCH2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27347 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP2-27347-img0002.jpg "Western Blot: Notch-2 Antibody [NBP2-27347] - Analysis of NOTCH2 in human brain lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-27347 at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NBP1-19125-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Notch2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NBP1-19125-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Notch-2 Antibody [NBP2-48913] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48913-img0004.jpg "Immunocytochemistry/Immunofluorescence: Notch-2 Antibody [NBP2-48913] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & plasma membrane.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP2-48913] - Staining of human testis shows strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NBP2-48913-img0005.jpg)