| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81030-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81029-0.2mg | |||
| Azide and BSA Free | NBP2-81029-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3244-SP | |||
![Immunohistochemistry: F4/80 Antibody (CI-A3-1) [NB600-404] - Oral treatment with L plantarum leads to recruitment of myeloid cells in kidney.Immunostaining of kidney sections from groups of treated mice, in the presence or absence of Leptospira infection using various leukocyte markers. We determined the number of F4/80+ T cells per millimeter squared of kidney. Data are mean SEM of 4-5 mice per group. Scale bar represents 400x. Statistics by two-tailed paired t-test *p < 0.05, *** p < 0.001. Data is representative of one of two experiments. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pntd.0005870) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunohistochemistry-NB600-404-img0052.jpg "Immunohistochemistry: F4/80 Antibody (CI-A3-1) [NB600-404] - Oral treatment with L plantarum leads to recruitment of myeloid cells in kidney.Immunostaining of kidney sections from groups of treated mice, in the presence or absence of Leptospira infection using various leukocyte markers. We determined the number of F4/80+ T cells per millimeter squared of kidney. Data are mean SEM of 4-5 mice per group. Scale bar represents 400x. Statistics by two-tailed paired t-test *p < 0.05, *** p < 0.001. Data is representative of one of two experiments. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pntd.0005870) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Lymph node stained for macrophages, red and B cells CD79b, green; nuclei are stained with DAPI, blue.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunocytochemistry-Immunofluorescence-NB600-404-img0045.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - A surface stain was performed on RAW 246.7 cells with F4/80 antibody (CI-A3-1) NB600-404AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 10 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0048.jpg)
![Immunohistochemistry-Paraffin: F4/80 Antibody (CI-A3-1) [NB600-404] - pNAPE-EcN reduces diet-induced hepatic inflammation. Representative images of liver sections immunostained for F4/80. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-37373-1), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunohistochemistry-Paraffin-NB600-404-img0050.jpg)
![Immunocytochemistry/Immunofluorescence: F4/80 Antibody (CI-A3-1) [NB600-404] - Analysis of monocytes cultured in M-CSF using F4/80 antibody. Primary antibody dilution: 1:200. Overnight incubation in RT. Image from verified customer review.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunocytochemistry-Immunofluorescence-NB600-404-img0053.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) - BSA Free [NB600-404] - Surface staining of F4/80 in CT26 colorectal carcinoma tumor model. Using Allophycocyanin conjugated version of the antibody (NB600-404APC). Image from verified customer review](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-BSA-Free-Flow-Cytometry-NB600-404-img0054.jpg)
![Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Frozen mouse spleen with F4/80 anti-mouse antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunohistochemistry-Frozen-NB600-404-img0046.jpg)
![Immunohistochemistry-Frozen: F4/80 Antibody (CI-A3-1) [NB600-404] - Mouse spleen stained with A: Rat anti Mouse. B: CI-A3-1 clone preincubated with 2 molar excess of human anti Idiotypic.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Immunohistochemistry-Frozen-NB600-404-img0047.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of J774 cells with F4/80 antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0037.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404] - Staining of mouse peritoneal macrophages with clone CI-A3-1 F4/80 antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0043.jpg)
![Flow Cytometry: F4/80 Antibody (CI-A3-1) [NB600-404]](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NB600-404-img0049.jpg)
![Immunohistochemistry: F4/80 Antibody (Cl:A3-1) [NB600-404] - Lymphocyte interaction inside the DC harboring scaffoldsDC scaffolds (beDC) or scaffolds only, placed in tumor bearing mice or DC-scaffold placed in normal mice were recovered two weeks post-implantation & processed for immunohistochemistry to demonstrate various cell types. (A) Relative size of the biomatrices harvested from various mice groups as depicted in the figure (far left panel). n = 3–5. The dotted line in ‘gross specimen’ differentiates the biomatrix from the reactive host-tissue. Representative images of biomatrix sections stained by the respective antibodies as indicated in the figure. The bar in the ‘gross structure’ is equivalent to 1 mm, whereas for other panels is equal to 100 μm. (B) Snapshots from Supplementary Movie S2 showing lymphocyte (green) movement & interaction with DCs (red) inside the biomatrix. The start point of the observation was set as the zero time point. (C) Lymphocyte division (white arrows) inside the biomatrix in the vicinity of DC (red). Representative snapshot from Supplementary Movie S3. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27223090), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-404_rat-monoclonal-f4-80-antibody-ci-a3-1-310202416171463.jpg)
![Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Mouse (Mus musculus) splenocytes were labelled ex vivo with a commercially available APC-labelled anti-F4/80 antibody and APE labelled anti-CD11b antibody and subject to flow-cytometry analysis (A), in which a small subpopulation of F4/80-CD11b positive cells may be observed. Subsets of commerical anti-F4/80 antibody-labelled splenocytes were also subsequently incubated with unlabelled versions of either the rat (Rattus norvegicus) IgG2b chimeric version (B) or mouse IgG2A chimeric (C) version of CI:A3-1. Loss of the F4/80-CD11b positive subpopulation may be observed, demonstrating displacement of the commercial antibody and the specificity of CI:A3-1](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NBP2-61613-img0004.jpg "Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Mouse (Mus musculus) splenocytes were labelled ex vivo with a commercially available APC-labelled anti-F4/80 antibody and APE labelled anti-CD11b antibody and subject to flow-cytometry analysis (A), in which a small subpopulation of F4/80-CD11b positive cells may be observed. Subsets of commerical anti-F4/80 antibody-labelled splenocytes were also subsequently incubated with unlabelled versions of either the rat (Rattus norvegicus) IgG2b chimeric version (B) or mouse IgG2A chimeric (C) version of CI:A3-1. Loss of the F4/80-CD11b positive subpopulation may be observed, demonstrating displacement of the commercial antibody and the specificity of CI:A3-1")
![Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Figure 1 murine bone marrow-derived macrophages (BMDMs) were pre-blocked with rat anti-mouse CD16 & CD32 (clone FCR-4G8) and stained with non-recombinant anti-F4/80 [Cl:A3-1] conjugated to Alexa Fluor 647 (AF647), all commercially available from competitors.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NBP2-61613-img0001.jpg)
![Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Figure 2 BMDMs were stained with recombinant anti-F4/80 [Cl:A3-1] or isotype control (anti-fluorescein [4-4-20 (enhanced)] IgG2b. Whilst in Figure 2 the highest fluorescence signal is seen with the recombinant anti-F4/80 IgG2 (the isotype of the original hybridoma-derived antibody), the isotype control IgG2b shows considerable signal overlap, indicative of binding of the antibody to Fc-receptors. This illustrates the importance of isotype controls in such experiments when using conventional antibody formats particularly when Fc-blocking reagents are incompatible with the system used due to reactivity with the secondary antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NBP2-61613-img0002.jpg)
![Flow Cytometry: F4/80 Antibody (CI:A3-1) [NBP2-61613] - Figure 3 BMDMs were stained with Fc Silent recombinant anti-F4/80 [Cl:A3-1] or isotype control (Fc Silent anti-fluorescein [4-4-20 (enhanced)] IgG2b. These were fluorescently labelled using the secondary antibody, goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor 647(AF647) commercially available from a competitor. The Fc silent format however overcomes this issue as seen in Figure 3, where the Fc silent recombinant anti-F4/80 yields a strong and distinct signal, whilst the isotype control shows no discernible difference to the background staining from the secondary antibody alone. Therefore, with Fc Silent reagents, no Fc-blocking products are required. [Data courtesy of Lewis Taylor.]](http://images.novusbio.com/fullsize/F4-80-Antibody-CI-A3-1-Flow-Cytometry-NBP2-61613-img0003.jpg)
![Western Blot: F4/80 Antibody (PSH0-85) [NBP3-32323] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 ug/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 ug/Lane) Lane 3: Rat spleen tissue lysate (70 heat) (40 ug/Lane) Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32323_rabbit-f4-80-mab-psh0-85-27820241631634.jpg "Western Blot: F4/80 Antibody (PSH0-85) [NBP3-32323] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate (no heat) (20 ug/Lane) Lane 2: L929 cell lysate (no heat) (negative) (20 ug/Lane) Lane 3: Rat spleen tissue lysate (70 heat) (40 ug/Lane) Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32323) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: F4/80 Antibody (PSH0-85) [NBP3-32323] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32323) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32323_rabbit-f4-80-mab-psh0-85-298202414262680.jpg)
![Immunohistochemistry: F4/80 Antibody (PSH0-85) [NBP3-32323] - Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling F4/80 with Rabbit anti-F4/80 antibody (NBP3-32323) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32323, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32323_rabbit-f4-80-mab-psh0-85-298202414161820.jpg)
![Western Blot: F4/80 Antibody (PSH01-87) [NBP3-32324] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/1,000 dilution. Lane 1: RAW264.7(no heat) cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32324_rabbit-f4-80-mab-psh01-87-278202415295528.jpg "Western Blot: F4/80 Antibody (PSH01-87) [NBP3-32324] - Western blot analysis of F4/80 on different lysates with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/1,000 dilution. Lane 1: RAW264.7(no heat) cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 102 kDa Observed band size: 160 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32324) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324] - Immunofluorescence analysis of paraffin-embedded mouse liver tissue labeling F4/80 with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32324, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32324_rabbit-f4-80-mab-psh01-87-29820241435214.jpg)
![Immunohistochemistry: F4/80 Antibody (PSH01-87) [NBP3-32324] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-F4/80 antibody (NBP3-32324) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32324) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32324_rabbit-f4-80-mab-psh01-87-298202414161825.jpg)
![Flow Cytometry: F4/80 Antibody (Cl:A3-1 (recombinant version)) [NBP2-81030] - Mouse (Mus musculus) splenocytes were labelled ex vivo with a commercially available APC-labelled anti-F4/80 antibody and APE labelled anti-CD11b antibody and subject to flow-cytometry analysis (A), in which a small subpopulation of F4/80-CD11b positive cells may be observed. Subsets of commerical anti-F4/80 antibody-labelled splenocytes were also subsequently incubated with unlabelled versions of either the rat ( Rattus norvegicus ) IgG2b chimeric version (NBP2-81030, B) or mouse IgG2A chimeric (C) version of CI:A3-1. Loss of the F4/80-CD11b positive subpopulation may be observed, demonstrating displacement of the commercial antibody and the specificity of CI:A3-1.](http://images.novusbio.com/fullsize/F4-80-Antibody-Cl-A3-1-recombinant-version-Flow-Cytometry-NBP2-81030-img0002.jpg "Flow Cytometry: F4/80 Antibody (Cl:A3-1 (recombinant version)) [NBP2-81030] - Mouse (Mus musculus) splenocytes were labelled ex vivo with a commercially available APC-labelled anti-F4/80 antibody and APE labelled anti-CD11b antibody and subject to flow-cytometry analysis (A), in which a small subpopulation of F4/80-CD11b positive cells may be observed. Subsets of commerical anti-F4/80 antibody-labelled splenocytes were also subsequently incubated with unlabelled versions of either the rat ( Rattus norvegicus ) IgG2b chimeric version (NBP2-81030, B) or mouse IgG2A chimeric (C) version of CI:A3-1. Loss of the F4/80-CD11b positive subpopulation may be observed, demonstrating displacement of the commercial antibody and the specificity of CI:A3-1.")
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0006.jpg "Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Splenocytes isolated from a IL1R-/- mouse (IL1b receptor KO) were stained with and CD45-e450 (1:100 in FACS buffer) for 30 minutes. Cells were then washed and analyzed by flow cytometry. Left panel shows the isotype control. This image was submitted via a customer review. Image from the PE version of this antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Frozen: F4/80 Antibody (BM8) [NBP1-60140] - Red: F4/80, Green: CD31/PECAM-1, Blue: DAPI+ nuclei. Frozen mouse lung tissues of WT B6 and IL1R--/- (knockout) mice were blocked with 1% BSA in PBS and were double stained with PE-conjugated F4/80 and Alex647-conjugated CD31/PECAM-1 antibody (MEC 7.46) for 2 hours at the room temperature. This image was submitted via a customer review. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-Frozen-NBP1-60140-img0007.jpg)
![Immunohistochemistry: F4/80 Antibody (BM8) [NBP1-60140] - Expression on macrophages in mouse liver. Staining of frozen tissue section with antibody BM8.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Immunohistochemistry-NBP1-60140-img0003.jpg)
![Flow Cytometry: F4/80 Antibody (BM8) [NBP1-60140] - Analysis using the FITC conjugate of NBP1-60140. Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 FITC.](http://images.novusbio.com/fullsize/F4-80-Antibody-BM8-Flow-Cytometry-NBP1-60140-img0005.jpg)
