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![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining in human liver and testis tissues . Corresponding MAOB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining in human liver and testis tissues . Corresponding MAOB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Monoamine Oxidase B Antibody [NBP1-87493] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-87493-img0006.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-87493] - Analysis in human liver tissue.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-87493-img0005.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0008.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human liver shows moderate to strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0009.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-87493] - Staining of human testis shows moderate to strong granular cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-87493-img0010.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-32426-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32426-img0011.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0010.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-32426-img0007.jpg)
![ELISA: Monoamine Oxidase B Antibody [NBP1-32426] - ELISA detection of MAOB using Monoamine Oxidase B antibody [N2C3] for capture at a concentration of 2.5 ug/mL and monoamine oxidase B for detection at a concentration of 0.5 ug/mL.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-ELISA-NBP1-32426-img0006.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] (NBP1-32426) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32426_rabbit-polyclonal-monoamine-oxidase-b-antibody-910202420134011.jpg)
![Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Western-Blot-NBP3-15411-img0004.jpg "Western Blot: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Analysis of extracts of various cell lines, using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Mouse testis using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Immunohistochemistry-Paraffin-NBP3-15411-img0003.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Rat lung using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Immunohistochemistry-Paraffin-NBP3-15411-img0001.jpg)
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Human colon using Monoamine Oxidase B (MAOB) Rabbit mAb (NBP3-15411) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-5M8A5-Immunohistochemistry-Paraffin-NBP3-15411-img0002.jpg)
![Simple Western: Monoamine Oxidase B Antibody (5M8A5) [NBP3-15411] - Monoamine Oxidase B Antibodies (NBP3-15411), ProteinSimple Western Blot on Jess Instrument. 1 microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp3-15411_rabbit-monoamine-oxidase-b-mab-5m8a5-simple-western-2042023112630.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Mice were pretreated with 5ECdsAP1 aptamer before amphetamine application; tissue samples collected (n = 4 per group) from the VTA at 90 (60 + 30 min) and 180 (60 + 30 + 90 min) minutes after amphetamine. Protein was obtained for Western blot quantitation of MAO-A level using Actin (lower molecular fragments) as a reference shown in lanes 2-4 (90 min samples) or lane 5-6 (180 min). Total protein (10 ug) was used for all lanes except lanes 7 & 9. The blot was stripped and used for MAO-B. Lane 1: molecular size marker of 10 KD ladder; lane 2: 5ECdsAP1 (4 nmol/kg, icv, 1/2 dose); lanes 3 & 5: saline (2 ul, icv); lanes 4 & 6: 5ECdsAP1 (8 nmol/kg, icv); lanes 7, 8, 9 are controls of naive mice with no aptamer or amphetamine (5, 10 and 20 ug protein). Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/23/1/21), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-59569-img0007.jpg "Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Mice were pretreated with 5ECdsAP1 aptamer before amphetamine application; tissue samples collected (n = 4 per group) from the VTA at 90 (60 + 30 min) and 180 (60 + 30 + 90 min) minutes after amphetamine. Protein was obtained for Western blot quantitation of MAO-A level using Actin (lower molecular fragments) as a reference shown in lanes 2-4 (90 min samples) or lane 5-6 (180 min). Total protein (10 ug) was used for all lanes except lanes 7 & 9. The blot was stripped and used for MAO-B. Lane 1: molecular size marker of 10 KD ladder; lane 2: 5ECdsAP1 (4 nmol/kg, icv, 1/2 dose); lanes 3 & 5: saline (2 ul, icv); lanes 4 & 6: 5ECdsAP1 (8 nmol/kg, icv); lanes 7, 8, 9 are controls of naive mice with no aptamer or amphetamine (5, 10 and 20 ug protein). Image collected and cropped by CiteAb from the following publication (//www.jbiomedsci.com/content/23/1/21), licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-59569] - Human Liver tissue.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunohistochemistry-Paraffin-NBP1-59569-img0005.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Human heart.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NBP1-59569-img0006.jpg)
![Simple Western: Monoamine Oxidase B Antibody [NBP1-59569] - Monoamine Oxidase B Antibodies (NBP1-59569), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp1-59569_rabbit-polyclonal-monoamine-oxidase-b-antibody-simple-western-2042023113029.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NB100-2826-img0002.jpg "Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NB100-2826] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2826-img0003.jpg)
![Flow Cytometry: Monoamine Oxidase B Antibody [NB100-2826] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Flow-Cytometry-NB100-2826-img0005.jpg)
![Western Blot: Monoamine Oxidase B Antibody [NB100-2826] - Staining of Human Liver (A) with antibody at 0.1 ug/mL and Testes (B) and Breast Cancer (C) lysate with antibody at 0.3ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Antibody-Western-Blot-NB100-2826-img0001.jpg)
![Immunohistochemistry: Monoamine Oxidase B Antibody (9B6) [NBP3-26155] - Image of Monoamine Oxidase B Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26155_rabbit-monoamine-oxidase-b-mab-9b6-262202414471525.jpg "Immunohistochemistry: Monoamine Oxidase B Antibody (9B6) [NBP3-26155] - Image of Monoamine Oxidase B Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: Monoamine Oxidase B Antibody (9B6) [NBP3-26155] - Image of Monoamine Oxidase B Antibody (9B6) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26155_rabbit-monoamine-oxidase-b-mab-9b6-26220241522417.jpg)
![Western Blot: Monoamine Oxidase B Antibody (9B6) [NBP3-26155] - Positive Western Blot detected in: L02 whole cell lysate, HepG2 whole cell lysate. All lanes: Monoamine Oxidase B Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59, 47 kDa Observed band size: 59 kDa](http://images.novusbio.com/fullsize/nbp3-26155_rabbit-monoamine-oxidase-b-mab-9b6-282202411485834.jpg)
![ELISA: Human Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81160] - Samples were spiked with high concentrations of Human Monoamine Oxidase B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81160_human-monoamine-oxidase-b-elisa-kit-colorimetric-13220241681435.jpg "ELISA: Human Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81160] - Samples were spiked with high concentrations of Human Monoamine Oxidase B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81160] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81160_human-monoamine-oxidase-b-elisa-kit-colorimetric-4320248582580.jpg)
![ELISA: Mouse Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81161] - Samples were spiked with high concentrations of Mouse Monoamine Oxidase B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81161_mouse-monoamine-oxidase-b-elisa-kit-colorimetric-132202416143074.jpg "ELISA: Mouse Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81161] - Samples were spiked with high concentrations of Mouse Monoamine Oxidase B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81161] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81161_mouse-monoamine-oxidase-b-elisa-kit-colorimetric-432024963984.jpg)
![ELISA: Rat Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81162] - Samples were spiked with high concentrations of Rat Monoamine Oxidase B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-81162_rat-monoamine-oxidase-b-elisa-kit-colorimetric-132202416175625.jpg "ELISA: Rat Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81162] - Samples were spiked with high concentrations of Rat Monoamine Oxidase B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Monoamine Oxidase B ELISA Kit (Colorimetric) [NBP2-81162] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-81162_rat-monoamine-oxidase-b-elisa-kit-colorimetric-4320249133251.jpg)
![Western Blot: Monoamine Oxidase B Overexpression Lysate (Adult Normal) [NBL1-12842] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monoamine Oxidase B.](http://images.novusbio.com/fullsize/Monoamine-Oxidase-B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12842-img0002.jpg "Western Blot: Monoamine Oxidase B Overexpression Lysate (Adult Normal) [NBL1-12842] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Monoamine Oxidase B.")