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![Western Blot: MYST1 Antibody [NB100-55251] - Detection of human MOF/MYST1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody NB100-55251 (lot NB100-55251-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). MOF/MYST1 was also immunoprecipitated by a previous lot (lot NB100-55251-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/MYST1-Antibody-Western-Blot-NB100-55251-img0005.jpg "Western Blot: MYST1 Antibody [NB100-55251] - Detection of human MOF/MYST1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody NB100-55251 (lot NB100-55251-2) used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). MOF/MYST1 was also immunoprecipitated by a previous lot (lot NB100-55251-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![Immunohistochemistry-Paraffin: MYST1 Antibody [NB100-55251] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiMOF/MYST1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MYST1-Antibody-Immunohistochemistry-Paraffin-NB100-55251-img0003.jpg)
![Western Blot: MYST1 Antibody [NB100-55251] - Whole cell lysate (50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-MOF/MYST1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MYST1-Antibody-Western-Blot-NB100-55251-img0004.jpg)
![Western Blot: MYST1 Antibody (6U5Z6) [NBP3-16178] - Western blot analysis of extracts of various cell lines, using MYST1 Rabbit mAb (NBP3-16178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/MYST1-Antibody-6U5Z6-Western-Blot-NBP3-16178-img0003.jpg "Western Blot: MYST1 Antibody (6U5Z6) [NBP3-16178] - Western blot analysis of extracts of various cell lines, using MYST1 Rabbit mAb (NBP3-16178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: MYST1 Antibody (6U5Z6) [NBP3-16178] - Immunofluorescence analysis of U-2 OS cells using MYST1 Rabbit mAb (NBP3-16178) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MYST1-Antibody-6U5Z6-Immunocytochemistry-Immunofluorescence-NBP3-16178-img0002.jpg)
![Immunohistochemistry-Paraffin: MYST1 Antibody (6U5Z6) [NBP3-16178] - Immunohistochemistry of paraffin-embedded human appendix using MYST1 Rabbit mAb (NBP3-16178) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/MYST1-Antibody-6U5Z6-Immunohistochemistry-Paraffin-NBP3-16178-img0001.jpg)
![Immunohistochemistry: MYST1 Antibody (7E11) [NBP3-26184] - Image of MYST1 Antibody (7E11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26184_rabbit-myst1-mab-7e11-2722024847195.jpg "Immunohistochemistry: MYST1 Antibody (7E11) [NBP3-26184] - Image of MYST1 Antibody (7E11) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Immunocytochemistry/Immunofluorescence: MYST1 Antibody (7E11) [NBP3-26184] - Staining of HepG2 cell with MYST1 Antibody (7E11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 587-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26184_rabbit-myst1-mab-7e11-282202412475123.jpg)
![Immunoprecipitation: MYST1 Antibody [NB100-55252] - Detection of Human MOF/MYST1 on HeLa whole cell lysate using NB100-55252. MOF/MYST1 was also immunoprecipitated by rabbit anti-MOF/MYST1 antibodies NB100-55251 and NB100-55253.](http://images.novusbio.com/fullsize/MYST1-Antibody-Immunoprecipitation-NB100-55252-img0002.jpg "Immunoprecipitation: MYST1 Antibody [NB100-55252] - Detection of Human MOF/MYST1 on HeLa whole cell lysate using NB100-55252. MOF/MYST1 was also immunoprecipitated by rabbit anti-MOF/MYST1 antibodies NB100-55251 and NB100-55253.")
![Immunocytochemistry/Immunofluorescence: MYST1 Antibody [NBP2-56864] - Staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/MYST1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56864-img0001.jpg "Immunocytochemistry/Immunofluorescence: MYST1 Antibody [NBP2-56864] - Staining of human cell line MCF7 shows localization to nucleoplasm.")
![Western Blot: MYST1 Antibody [NBP3-12699] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:52 kDa](http://images.novusbio.com/fullsize/MYST1-Antibody-Western-Blot-NBP3-12699-img0001.jpg "Western Blot: MYST1 Antibody [NBP3-12699] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:52 kDaObserved band size:52 kDa")