Novus Biologicals products are now on bio-techne.com

Sort By:

Products (10)

page of 1

htra1 Products By Gene Name

10 Results for "htra1"

List

Grid

MAB2916

HTRA1/PRSS11 Antibody (275615)

(13 Publications)

100 μg

MAB2916

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB2916) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HTRA1/PRSS11 at approximately 51 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

$HtrA1 Protein Expression in hBC cell lines.Cells (as indicated) were extracted into nuclear (N) and cytoplasmic (C) fractions as described (Materials & Methods), and proteins were analyzed by Immunoblot analyses using the polyclonal antibody against human HtrA1. beta -actin was used as a loading control, and DEK was used to assess the nuclear/cytoplasmic fractionation (DEK is exclusively nuclear). The larger Mr bands seen in the MCF12A and MCF10A/Flp cell lines are consistent with dimers, trimers, etc., although this was not confirmed. MCF10A/Flp is the parental Flp-in cell line which was used to produce the various MCF10A/siRNA or Htra1 cell lines. Results are from a representative experiment. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22761798), licensed under a CC-BY license. Not internally tested by R&D Systems.$

$Characterization of MCF10A cell lines carrying HtrA1 siRNA and overexpression vectors.A random antisense oligonucleotide library was used to identify optimally accessible sites in HtrA1 mRNA. SiRNAs (short hairpin RNAs) were designed to target these sites, and stably transfected cell lines were developed from MCF10A cells (4 independent cell lines, designated MCF10A/siRNA1-4). A control cell line expressing an irrelevant siRNA (designated MCF10A/HPVsh) was also developed. In parallel, we also developed a cell line over-expressing HtrA1 (designated MCF10A/HtrA1). (A). Target sites empirically identified in HtrA1 mRNA. Identified domains within HtrA1 include: SS, signal sequence; IGFBP, IGF binding site; KI, Kazal Type I protease inhibitor domain; Trypsin-like protease domain; PDZ, PDZ binding domain. (B) Immunoblot analysis for HtrA1 protein. Cytoplasmic and nuclear protein fractions were prepared from the developed cell lines (as indicated), and were probed with a polyclonal antibody preparation directed against a region in the trypsin-like protease domain. As is evident, the reductions in HtrA1 protein levels were >90% compared with the various MCF10A and MCF10A/HPVsh cells. Right panel shows HtrA1 in concentrated culture medium. (C) Cells were plated and growth was measured over a 6 day period. Both of the MCF10A cell lines tested (MCF10A/siRNA1 and siRNA4) grew significantly faster than the control cells (p<0.01 at days 4 and 6). Over-expression of HtrA1 in the MCF10A/HtrA1 cells had no effect on cell growth rate. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22761798), licensed under a CC-BY license. Not internally tested by R&D Systems.$

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2916-SP

compare

(select up to 3 products from this page to compare)

NBP2-23869

HTRA1/PRSS11 Antibody

(2 Publications)

0.1 ml

NBP2-23869

Reactivity:

Hu, Mu, Rt, Bv, Ca, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Ca, Pm

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6518A

Alt Catalog# IMG-6518A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

2916-SE

Recombinant Human HTRA1 Protein, CF

(5 Publications)

20 μg

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP2-23869PEP

HTRA1/PRSS11 Antibody Blocking Peptide

0.1 mg

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP1-81654

HTRA1/PRSS11 Antibody

(1 Publication)

0.1 ml

NBP1-81654

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP3-27848

HTRA1/PRSS11 Antibody (SR4329)

100 ul

NBP3-27848

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBL1-11785

HTRA1/PRSS11 Overexpression Lysate

0.1 mg

NBL1-11785

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

NBP2-76585

Human HTRA1/PRSS11 ELISA Kit (Colorimetric)

1 Kit

NBP2-76585

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.313 - 20ng/mL

Sensitivity:

0.188ng/mL

Sample Type:

Serum, plasma and other biological fluids

compare

(select up to 3 products from this page to compare)

NBP3-28037

HTRA1/PRSS11 Antibody (FHTR2163)

100 μg

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP1-81654PEP

HTRA1/PRSS11 Recombinant Protein Antigen

0.1 ml

Reactivity:

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

page of 1

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

Visit our other sites

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Host

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Has Sample Size

Promotional Item

Species Glossary

htra1 Products By Gene Name

HTRA1/PRSS11 Antibody (275615)

HTRA1/PRSS11 Antibody

Recombinant Human HTRA1 Protein, CF

HTRA1/PRSS11 Antibody Blocking Peptide

HTRA1/PRSS11 Antibody

HTRA1/PRSS11 Antibody (SR4329)

HTRA1/PRSS11 Overexpression Lysate

Human HTRA1/PRSS11 ELISA Kit (Colorimetric)

HTRA1/PRSS11 Antibody (FHTR2163)

HTRA1/PRSS11 Recombinant Protein Antigen