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![Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Western-Blot-NB120-2788-img0019.jpg "Western Blot: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSP70 monoclonal antibody at a dilution of 1:1000 overnight at 4C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgM-HRP secondary antibody at a dilution of 1:20,000 for at least 1 hour.")
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using Monoclonal antibody (13D3) shows staining in NIH-3T3 cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0013.jpg)
![Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Prostate carcinoma tissue.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunohistochemistry-Paraffin-NB120-2788-img0022.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP70 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in HeLa cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 using HSC70 Monoclonal antibody (13D3) shows staining in MCF-7 cells. HSC70 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing HSC70 at a dilution of 1:20-1:200 C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunocytochemistry-Immunofluorescence-NB120-2788-img0012.jpg)
![Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Tonsil tissue.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunohistochemistry-Paraffin-NB120-2788-img0020.jpg)
![Immunohistochemistry-Paraffin: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Both normal and cancer biopsies of deparaffinized human Testis tissue.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunohistochemistry-Paraffin-NB120-2788-img0021.jpg)
![Immunoprecipitation: Hsp70 / Hsc70 Antibody (13D3) [NB120-2788] - Analysis of HSC70 was performed on HeLa cells. Antigen-antibody complexes were formed by incubating 500ug whole cell lysate with 2ul of HSC70 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50ul Protein A/G Plus Agarose, washed extensively, and eluted with Lane Marker Reducing Sample Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a HSC70 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with goat anti-mouse IgM-HRP secondary antibody for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.](http://images.novusbio.com/fullsize/Hsp70-Hsc70-Antibody-13D3-Immunoprecipitation-NB120-2788-img0023.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Western-Blot-NBP1-97868-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1B5-Immunohistochemistry-Paraffin-NBP1-97868-img0001.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NB100-41377-img0006.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41377-img0005.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NB100-41377-img0008.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - A. 30 ug 293T whole cell lysate/extractB. 30 ug A431whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % SDS-PAGE HSC70 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NBP2-16882-img0013.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - A. 30 ug 293T whole cell lysate/extractB. 30 ug A431whole cell lysate/extractC. 30 ug HeLa whole cell lysate/extractD. 30 ug HepG2 whole cell lysate/extract7.5 % SDS-PAGE HSC70 antibody dilution: 1:1000")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSC70 protein stained by HSC70 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16882-img0012.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse prostate by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0010.jpg)
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: Neuro2A B: GL261 C: C8D30 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0001.jpg)
![Western Blot: Hsc70 Antibody [NBP2-16882] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16882-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsc70 Antibody [NBP2-16882] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0003.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16882] - HSC70 antibody detects HSC70 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. HSC70 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16882-img0005.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Western-Blot-NBP2-12880-img0010.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Western Blot analysis of Human Cell lysates showing detection of HSPA8/HSC71/Hsc70 protein using Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody, Clone 1F2-H5 (NBP2-12880). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HSPA8/HSC71/Hsc70 Monoclonal Antibody (NBP2-12880) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. 1: mix of 10 different human cell lines, 2: Hsp72 recombinant protein, and 3: Hsc70(Hsp73) recombinant protein.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 Monoclonal Antibody, Clone 1F2-H5. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degrees C. Primary Antibody: Mouse Anti-Hsc70 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Bright cytoplasmic staining, duller nuclear staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Mouse (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (1F2-H5) [NBP2-12880] - Analysis using Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody, Clone 1F2-H5. Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsc70 (Hsp73) Monoclonal Antibody at 1:100 for 12 hours at 4 degrees C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Melanosome. Localizes to nucleus upon heat shock. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsc70 (Hsp73) Antibody. (C) Composite.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-1F2-H5-Immunocytochemistry-Immunofluorescence-NBP2-12880-img0007.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Western-Blot-NBP2-45746-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 .](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Immunocytochemistry-Immunofluorescence-NBP2-45746-img0004.jpg)
![Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Immunohistochemistry-NBP2-45746-img0001.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) [NBP2-45746] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Western-Blot-NBP2-45746-img0003.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16881-img0003.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Immunohistochemistry-Paraffin-NBP2-16881-img0004.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (20 ug of whole cell lysate) A: Raw264. 7 NBP2-16881 diluted at 1:2000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16881-img0001.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NBP2-16881] - Sample (30 ug of whole cell lysate) A: A431 B: H1299 C: Hela D: Hep G2 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Hsc70-Antibody-Western-Blot-NBP2-16881-img0002.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Western-Blot-NBP2-70962-img0002.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 .](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70962-img0003.jpg)
![Immunohistochemistry: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70962-img0004.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody (OTI1H3) - Azide and BSA Free [NBP2-70962] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-OTI1H3-Azide-and-BSA-Free-Western-Blot-NBP2-70962-img0001.jpg)
![SDS-Page: Recombinant Human HSPA8/HSC71/Hsc70 Protein [NBP1-30278] - Analysis of Human protein.](http://images.novusbio.com/fullsize/Recombinant-Human-HSPA8-HSC71-Hsc70-Protein-SDS-Page-NBP1-30278-img0001.jpg "SDS-Page: Recombinant Human HSPA8/HSC71/Hsc70 Protein [NBP1-30278] - Analysis of Human protein.")
![Western Blot: HSC70 Antibody (plant) [NBP1-97498] - Analysis of HSC70 (plant, ER, BiP): Lane 1: MW marker, Lane 2: Apple, Lane 3: Carrot, Lane 4: Spinach.](http://images.novusbio.com/fullsize/HSC70-Antibody-plant-Western-Blot-NBP1-97498-img0001.jpg "Western Blot: HSC70 Antibody (plant) [NBP1-97498] - Analysis of HSC70 (plant, ER, BiP): Lane 1: MW marker, Lane 2: Apple, Lane 3: Carrot, Lane 4: Spinach.")