| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2024AF405 | |||
| Alexa Fluor 488 | NB21-2024AF488 | |||
| Alexa Fluor 647 | NB21-2024AF647 | |||
| Alexa Fluor 700 | NB21-2024AF700 | |||
| Biotin | NB21-2024B | |||
| DyLight 405 | NB21-2024V | |||
| DyLight 488 | NB21-2024G | |||
| DyLight 550 | NB21-2024R | |||
| DyLight 650 | NB21-2024C | |||
| FITC | NB21-2024F | |||
| HRP | NB21-2024H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2024SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2077AF405 | |||
| Alexa Fluor 488 | NB21-2077AF488 | |||
| Alexa Fluor 647 | NB21-2077AF647 | |||
| Alexa Fluor 700 | NB21-2077AF700 | |||
| Biotin | NB21-2077B | |||
| DyLight 405 | NB21-2077V | |||
| DyLight 488 | NB21-2077G | |||
| DyLight 550 | NB21-2077R | |||
| DyLight 650 | NB21-2077C | |||
| FITC | NB21-2077F | |||
| HRP | NB21-2077H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2077SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-2089AF405 | |||
| Alexa Fluor 488 | NB21-2089AF488 | |||
| Alexa Fluor 647 | NB21-2089AF647 | |||
| Alexa Fluor 700 | NB21-2089AF700 | |||
| Biotin | NB21-2089B | |||
| DyLight 405 | NB21-2089V | |||
| DyLight 488 | NB21-2089G | |||
| DyLight 550 | NB21-2089R | |||
| DyLight 650 | NB21-2089C | |||
| FITC | NB21-2089F | |||
| HRP | NB21-2089H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2090SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-2044V | |||
| FITC | NB21-2044F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-2044SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80771 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5215-SP | |||
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0010.jpg "Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in HeLa histone prep.](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0009.jpg)
![Histone H4 [ac Lys5] Antibody [NB21-2024]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-NB21-2024-img0011.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0008.jpg "Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Western Blot using NB21-2077 of porcine whole ovarian homogenate. Image submitted via verified customer review.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody [NB21-2077] - Histone H4 K16ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac--Lys16]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2077-img0005.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - WB analysis of H4K16ac in 1) HeLa histone prep lysate and 2) NIH 3T3 histone prep lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Western-Blot-NB21-2077-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys16] Antibody [NB21-2077] - Dot blot analysis of H4K16ac in picomoles of protein.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-Dot-Blot-NB21-2077-img0004.jpg)
![Histone H4 [ac Lys16] Antibody [NB21-2077]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys16]-Antibody-NB21-2077-img0006.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0006.jpg "Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of Histone H4K20me2 in C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Histone H4 K20me2 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2089-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-2089 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NB21-2089-img0009.jpg)
![Western Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Detection of H4K20me2 in HeLa histone preps.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Western-Blot-NB21-2089-img0003.jpg)
![Dot Blot: Histone H4 [Dimethyl Lys20] Antibody [NB21-2089] - Analysis of H4K20me2 using peptides stated above in 4, 3, 2 and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-Dot-Blot-NB21-2089-img0001.jpg)
![Histone H4 [Dimethyl Lys20] Antibody [NB21-2089]](http://images.novusbio.com/fullsize/Histone-H4-[Dimethyl-Lys20]-Antibody-NB21-2089-img0010.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NB21-2090-img0002.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.")
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NB21-2090-img0001.jpg)
![Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Simple-Western-NB21-2090-img0003.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NB21-2044-img0006.jpg "Western Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - WB analysis of H4 K8ac in 1) HeLa histone prep 2) 3T3 histone prep and 3) C. elegans embryo lysate.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NB21-2044] - H4 K8ac antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2044-img0002.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NB21-2044] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-2044 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NB21-2044-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NB21-2044] - Dot Blot analysis of H4 K8ac using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NB21-2044-img0004.jpg)
![Histone H4 [ac Lys8] Antibody [NB21-2044]](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-NB21-2044-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Chromatin-Immunoprecipitation-NBP1-30091-img0001.jpg "Chromatin Immunoprecipitation: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - HeLa extracts.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with Histone H4 [Monomethyl Lys20] Antibody [5E10-D8] (NBP1-30091) at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0009.jpg)
![Western Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 meIK20 in HeLa histone lysates.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Western-Blot-NBP1-30091-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Histone H4 [methyl Lys20] staining in Hela cells detected with a DyLight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Immunocytochemistry-Immunofluorescence-NBP1-30091-img0006.jpg)
![Dot Blot: Histone H4 [Monomethyl Lys20] Antibody (5E10-D8) [NBP1-30091] - Analysis of Histone H4 me1K20 with 50 pmol or 10 pmol of peptides corresponding to amino acids 12-31 of Histone H4 bearing unmodified, monomethylated, dimethylated or trimethylated K20.](http://images.novusbio.com/fullsize/Histone-H4-Monomethyl-Lys20-Antibody-5E10-D8-Dot-Blot-NBP1-30091-img0002.jpg)
![Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Western-Blot-NBP2-42863-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42863-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0006.jpg)
![<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Chromatin-Immunoprecipitation-NBP2-42863-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunohistochemistry-Paraffin-NBP2-42863-img0005.jpg)
![Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Dot-Blot-NBP2-42863-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs) B. Control with 2.5 ug of pre-immune rabbit IgG C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys8]-Antibody-Immunoprecipitation-NBP2-42863-img0007.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-42862-img0007.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42862-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0006.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0003.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunohistochemistry-Paraffin-NBP2-42862-img0005.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0006.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - (Top): Human osteosarcoma (U2OS) cells were stained with and DAPI. Cells were fixed with ice cold methanol for 10' and blocked with PBS/TX-100 containing 5% normal goat serum. Figure 6A: cells were immunofluorescently labeled with (left) diluted 1:300 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. (Bottom): Staining of the cells with the after incubation of the antibody with blocking peptide, concentration: 5 ng/ul.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59259-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the antibody against H4K20me3 and optimized PCR primer sets for qPCR. Sheared chromatin from 10,000 cells was used per ChIP experiment. The antibody was tested at two different dilutions of 1:500 and 1:100. The pre-immune serum (PI) was used as a negative control. Quantitative PCR was performed using primer sets for the satellite repeat Sat2 as a positive control and for the promoter of the house keeping gene c-fos, as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H4K20me3 is preferably present at heterochromatin.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59259-img0004.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone (acid) extracts of NB4 cells were analysed by Western blot using the Diagenode antibody against H4K20me3, diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0002.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Dot Blot was used to check the specificity of the antibody against H4K20me3 with peptides containing other modifications of histone H3 and H4. Other histone modifications include monoand dimethylation of the same lysine and mono-, di- and trimethylation of lysines 9, 27 and 36 of H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0001.jpg)
![ELISA: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H4K20me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:700.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-59259-img0003.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - A Dot Blot analysis was performed to test the cross reactivity of Histone H4 [Trimethyl Lys20] Antibody with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0007.jpg)
![Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Western-Blot-NBP2-42858-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Un-treated (-) and treated (+, 0.4uM Trichostatin A treatment for 18hr) HeLa whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4 (acetyl Lys16) antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys16) protein stained by Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunocytochemistry-Immunofluorescence-NBP2-42858-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat kidney. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse brain. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0001.jpg)
![Immunohistochemistry-Paraffin: Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Analysis of mouse kidney. Histone H4 (acetyl Lys16) antibody [1271] diluted at 1:500.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-Immunohistochemistry-Paraffin-NBP2-42858-img0002.jpg)
![Histone H4 [ac Lys16] Antibody (1271) [NBP2-42858] - Rat brain. Histone H4K16ac (Acetyl Lys16) stained by Histone H4K16ac (Acetyl Lys16) antibody [1271] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H4-ac-Lys16-Antibody-1271-NBP2-42858-img0006.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NBP2-59254] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Western-Blot-NBP2-59254-img0001.jpg "Western Blot: Histone H4 [ac Lys5] Antibody [NBP2-59254] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H4K5ac. The antibody was diluted 1:500 in TBS-Tween containing 5% skimmed milk. The marker (kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NBP2-59254] - HeLa cells were stained with the antibody against H4K5ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H4K5ac antibody (left) diluted 1:500 in blocking solution followed by an antirabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Immunofluorescence-NBP2-59254-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NBP2-59254] - ChIP assays were performed using human HeLa cells, the antibody against H4K5ac and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP-seq kit on sheared chromatin from 1 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Chromatin-Immunoprecipitation-NBP2-59254-img0003.jpg)
![ELISA: Histone H4 [ac Lys5] Antibody [NBP2-59254] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H4K5ac in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:8,900.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-ELISA-NBP2-59254-img0002.jpg)
![Dot Blot: Histone H4 [ac Lys5] Antibody [NBP2-59254] - One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[ac-Lys5]-Antibody-Dot-Blot-NBP2-59254-img0005.jpg)