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![Western Blot: Flightless I Antibody [NBP1-87925] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP1-87925-img0006.jpg "Western Blot: Flightless I Antibody [NBP1-87925] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue")
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87925] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87925-img0007.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0011.jpg)
![Western Blot: Flightless I Antibody [NBP1-87925] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP1-87925-img0004.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0001.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human cervix, uterine shows strong cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0008.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0009.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87925] - Staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87925-img0010.jpg)
![Western Blot: Flightless I Antibody [NBP1-87926] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP1-87926-img0007.jpg "Western Blot: Flightless I Antibody [NBP1-87926] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87926] - Staining of human cell line U-2 OS shows localization to nucleoplasm, cytosol & microtubule organizing center.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87926-img0010.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0014.jpg)
![Western Blot: Flightless I Antibody [NBP1-87926] - Analysis in human cell line A-549.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP1-87926-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Flightless I Antibody [NBP1-87926] - Staining of human cell line U-2 OS shows positivity in cytoplasm, microtubule organizing center and nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87926-img0009.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0001.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0011.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human gastrointestinal shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0012.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP1-87926] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP1-87926-img0013.jpg)
![Western Blot: Flightless I Antibody [NBP3-03712] - Analysis of extracts of various cell lines, using Flightless I antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP3-03712-img0004.jpg "Western Blot: Flightless I Antibody [NBP3-03712] - Analysis of extracts of various cell lines, using Flightless I antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded mouse heart using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0003.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded rat heart using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0001.jpg)
![Immunohistochemistry-Paraffin: Flightless I Antibody [NBP3-03712] - Paraffin-embedded human colon carcinoma using Flightless I antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Immunohistochemistry-Paraffin-NBP3-03712-img0002.jpg)
![Western Blot: Flightless I Antibody [NBP3-32341] - Western blot analysis of Flightless I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32341, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/nbp3-32341_rabbit-flightless-i-pab-2782024163164.jpg "Western Blot: Flightless I Antibody [NBP3-32341] - Western blot analysis of Flightless I on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32341, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse lung tissue lysate Lane 2: MCF-7 cell lysate")
![Immunohistochemistry: Flightless I Antibody [NBP3-32341] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Flightless I antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32341, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32341_rabbit-flightless-i-pab-298202414115421.jpg)
![Western Blot: Flightless I Antibody [NBP2-87448] - Host: Rabbit. Target Name: FLII. Sample Tissue: Human K562 Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/Flightless-I-Antibody-Western-Blot-NBP2-87448-img0001.jpg "Western Blot: Flightless I Antibody [NBP2-87448] - Host: Rabbit. Target Name: FLII. Sample Tissue: Human K562 Whole Cell lysates. Antibody Dilution: 1ug/ml")
![Western Blot: Flightless I Overexpression Lysate (Adult Normal) [NBP2-10791] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Flightless I.](http://images.novusbio.com/fullsize/Flightless-I-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10791-img0001.jpg "Western Blot: Flightless I Overexpression Lysate (Adult Normal) [NBP2-10791] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Flightless I.")
![ELISA: Human Flightless I - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31674] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31674_human-flightless-i-ready-to-use-elisa-kit-colorimetric-20620241531480.png "ELISA: Human Flightless I - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31674] - Standard Curve Reference")
![ELISA: Human Flightless I ELISA Kit (Colorimetric) [NBP3-31673] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31673_human-flightless-i-elisa-kit-colorimetric-206202415273796.png "ELISA: Human Flightless I ELISA Kit (Colorimetric) [NBP3-31673] - Standard Curve Reference")