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![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-47477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47477-img0004.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0012.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0006.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0010.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0011.jpg)
![Western Blot: Dynamin 2 Antibody [NB300-617] - Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti-Dynamin II Rabbit polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) )Secondary Antibody, HRP conjugate. A band ~98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NB300-617-img0006.jpg "Western Blot: Dynamin 2 Antibody [NB300-617] - Western blot analysis of Dynamin II was performed using whole cell lysates and tissue lysate of SH-SY5Y (Lane 1), PC-12 (Lane 2), Neuro-2a (Lane 3), SK-N-AS (Lane 4), U- 87 MG (Lane 5) and Rat Brain (Lane 6). The blot was probed with Anti-Dynamin II Rabbit polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) )Secondary Antibody, HRP conjugate. A band ~98 kDa corresponding to Dynamin II was observed across cell lines and tissue lysate tested. A non specific band of ~48 KDa was observed across cell lines and tissue lysate tested except for Neuro-2a cell line.")
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin 2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Trito(TM) X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Dynamin 2 Polyclonal Antibody in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Recombinant Secondary Antibody, Alexa Fluor(R) 488 conjugate, for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong(TM) Diamond Antifade Mountant with DAPI F-actin (Panel c: Red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing Cytoskeletal and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NB300-617-img0004.jpg)
![Dynamin 2 Antibody [NB300-617] - Immunofluorescence analysis of Dynamin II was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton(TM) X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Dynamin II Rabbit Polyclonal Antibody at 2 ug/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal(TM) Secondary Antibody, Alexa Fluor(R) 488 conjugate for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade(R) Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor(R) 555 Rhodamine Phalloidin . Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-NB300-617-img0005.jpg)
![Western Blot: Dynamin 2 Antibody [NBP2-16244] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-16244-img0006.jpg "Western Blot: Dynamin 2 Antibody [NBP2-16244] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-16244] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16244-img0002.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-16244] - Human breast carcinoma.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-16244-img0003.jpg)
![Western Blot: Dynamin 2 Antibody [NBP2-16244] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-16244-img0004.jpg)
![Western Blot: Dynamin 2 Antibody [NBP2-16244] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-16244-img0005.jpg)
![Western Blot: Dynamin 2 Antibody [NBP2-16244] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dynamin 2 antibody [N1N3] (NBP2-16244) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16244_rabbit-polyclonal-dynamin-2-antibody-91020242011462.jpg)
![Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Western-Blot-NBP2-61757-img0004.jpg "Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis of Hela cells using mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Flow-Cytometry-NBP2-61757-img0002.jpg)
![Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mAb against human Dynamin-2 (AA: 520-744) recombinant protein. (Expected MW is 28 kDa)](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Western-Blot-NBP2-61757-img0003.jpg)
![Western Blot: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Analysis using Dynamin-2 mouse mAb against Hela (1), U251 (2), K562 (3), Jurkat (4), and Ramos (5) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-Western-Blot-NBP2-61757-img0005.jpg)
![ELISA: Dynamin 2 Antibody (3F5F3) [NBP2-61757] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5F3-ELISA-NBP2-61757-img0001.jpg)
![Western Blot: Dynamin 2 Antibody (1D8) [NBP3-26615] - Positive Western Blot detected in: HL60 whole cell lysate, Rat brain tissue. All lanes: Dynamin 2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 99, 98 kDa Observed band size: 99 kDa](http://images.novusbio.com/fullsize/nbp3-26615_rabbit-dynamin-2-mab-1d8-282202411485828.jpg "Western Blot: Dynamin 2 Antibody (1D8) [NBP3-26615] - Positive Western Blot detected in: HL60 whole cell lysate, Rat brain tissue. All lanes: Dynamin 2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 99, 98 kDa Observed band size: 99 kDa")
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody (1D8) [NBP3-26615] - Staining of Hela Cells with Dynamin 2 Antibody (1D8) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26615_rabbit-dynamin-2-mab-1d8-28220241312223.jpg)
![Flow Cytometry: Dynamin 2 Antibody (1D8) [NBP3-26615] - Overlay histogram showing Hela cells stained with Dynamin 2 Antibody (1D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26615_rabbit-dynamin-2-mab-1d8-282202414183688.jpg)
![Western Blot: Dynamin 2 Antibody (3F5C7) [NBP2-61758] - Analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5C7-Western-Blot-NBP2-61758-img0003.jpg "Western Blot: Dynamin 2 Antibody (3F5C7) [NBP2-61758] - Analysis using Dynamin-2 mAb against HEK293 (1) and Dynamin-2 (AA: 520-744)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: Dynamin 2 Antibody (3F5C7) [NBP2-61758] - Analysis using Dynamin-2 mAb against human Dynamin-2 (AA: 520-744) recombinant protein. (Expected MW is 28 kDa)](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5C7-Western-Blot-NBP2-61758-img0002.jpg)
![Western Blot: Dynamin 2 Antibody (3F5C7) [NBP2-61758] - Analysis using Dynamin-2 mouse mAb against U251 (1), Hela (2), and K562 (3) cell lysate.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5C7-Western-Blot-NBP2-61758-img0004.jpg)
![ELISA: Dynamin 2 Antibody (3F5C7) [NBP2-61758] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-3F5C7-ELISA-NBP2-61758-img0001.jpg)
![Western Blot: Dynamin 2 Antibody [NBP3-38480] - Western blot analysis of various lysates using Dynamin 2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-38480_rabbit-dynamin-2-pab-101220241224149.jpg "Western Blot: Dynamin 2 Antibody [NBP3-38480] - Western blot analysis of various lysates using Dynamin 2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![Immunocytochemistry/ Immunofluorescence: Dynamin 2 Antibody [NBP3-38480] - Immunofluorescence analysis of NIH-3T3 cells using Dynamin 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38480_rabbit-dynamin-2-pab-1012202411592830.jpg)
![Western Blot: Dynamin 2 Antibody (6C9) [H00001785-M01] - DNM2 monoclonal antibody (M01), clone 6C9 Analysis of DNM2 expression in HeLa.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-6C9-Western-Blot-H00001785-M01-img0004.jpg "Western Blot: Dynamin 2 Antibody (6C9) [H00001785-M01] - DNM2 monoclonal antibody (M01), clone 6C9 Analysis of DNM2 expression in HeLa.")
![Sandwich ELISA: Dynamin 2 Antibody (6C9) [H00001785-M01] - Detection limit for recombinant GST tagged DNM2 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-6C9-Sandwich-ELISA-H00001785-M01-img0003.jpg)
![SDS-Page: Recombinant Human Dynamin 2 Protein [H00001785-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Dynamin-2-Protein-SDS-Page-H00001785-P01-img0002.jpg "SDS-Page: Recombinant Human Dynamin 2 Protein [H00001785-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry;Dynamin 2 Antibody[NBP3-29491]Detection of human Dynamin 2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Dynamin 2 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-29492_rabbit-dynamin-2-pab-78202410343510.jpg "Immunohistochemistry;Dynamin 2 Antibody[NBP3-29491]Detection of human Dynamin 2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Dynamin 2 used at a dilution of 1:1,000 . Detection: DAB")
![Western Blot: Dynamin 2 Overexpression Lysate (Adult Normal) [NBP2-11313] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dynamin 2.](http://images.novusbio.com/fullsize/Dynamin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11313-img0001.jpg "Western Blot: Dynamin 2 Overexpression Lysate (Adult Normal) [NBP2-11313] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dynamin 2.")