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![Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-231-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: DIAPH1 Antibody [NB600-231] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.66 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: DIAPH1 Antibody [NB600-231] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Diaphanous used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-NB600-231-img0010.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DIAPH1 Antibody [NB600-231] - Whole cell lysate (50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 ug/ml.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-231-img0008.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NB600-231] - FFPE section of human ovarian carcinoma. Affinity purified rabbit anti-Diaphanous1 used at a dilution of 1:100.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NB600-231-img0006.jpg)
![Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-230-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: DIAPH1 Antibody [NB600-230] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Diaphanous 1 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Diaphanous 1 was also immunoprecipitated by a prior lot of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 1 second (B).")
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Western Blot: DIAPH1 Antibody [NB600-230] - Whole cell lysate (10 or 50 ug/lane) from untreated or siRNA treated 293T cells. Antibody used at 0.1 and 0.04 ug/ml.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NB600-230-img0002.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0008.jpg "Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0005.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human lung shows strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0006.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0007.jpg)
![Immunohistochemistry-Paraffin: DIAPH1 Antibody [NBP1-85246] - Staining of human spleen shows moderate cytoplasmic positivity in sinusoids.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Immunohistochemistry-Paraffin-NBP1-85246-img0009.jpg)
![Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of 293T cells, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-6N10D0-Western-Blot-NBP3-15410-img0001.jpg "Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of 293T cells, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: DIAPH1 Antibody (6N10D0) [NBP3-15410] - Western blot analysis of extracts of various cell lines, using DIAPH1 Rabbit mAb (NBP3-15410) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-6N10D0-Western-Blot-NBP3-15410-img0002.jpg)
![Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-Western-Blot-NBP1-47202-img0002.jpg "Western Blot: DIAPH1 Antibody [NBP1-47202] - Analysis of NIH3T3 lysate (35ug protein in RIPA buffer) using NBP1-47202 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: DIAPH1 Overexpression Lysate (Adult Normal) [NBP2-08476] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DIAPH1.](http://images.novusbio.com/fullsize/DIAPH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08476-img0001.jpg "Western Blot: DIAPH1 Overexpression Lysate (Adult Normal) [NBP2-08476] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DIAPH1.")
![Western Blot: DIAPH1 Overexpression Lysate (Adult Normal) [NBP2-09814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DIAPH1.](http://images.novusbio.com/fullsize/DIAPH1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09814-img0001.jpg "Western Blot: DIAPH1 Overexpression Lysate (Adult Normal) [NBP2-09814] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DIAPH1.")
![Western Blot:DIAPH1 Antibody - Azide and BSA Free-NBP3-03284-Analysis of lysates from wild type (WT) and DIAPH1 knockdown (KD) 293T cells, using [KD Validated] DIAPH1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/antibody/nbp3-03284_rabbit-polyclonal-diaph1-antibody-western-blot-15102024132324..jpg "Western Blot:DIAPH1 Antibody - Azide and BSA Free-NBP3-03284-Analysis of lysates from wild type (WT) and DIAPH1 knockdown (KD) 293T cells, using [KD Validated] DIAPH1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: DIAPH1 Antibody (1A8) [H00001729-M02] - Detection against Immunogen (37.18 KDa) .](http://images.novusbio.com/fullsize/DIAPH1-Antibody-1A8-Western-Blot-H00001729-M02-img0001.jpg "Western Blot: DIAPH1 Antibody (1A8) [H00001729-M02] - Detection against Immunogen (37.18 KDa) .")
![Sandwich ELISA: DIAPH1 Antibody (5A8) [H00001729-M01] - Detection limit for recombinant GST tagged DIAPH1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DIAPH1-Antibody-5A8-Sandwich-ELISA-H00001729-M01-img0002.jpg "Sandwich ELISA: DIAPH1 Antibody (5A8) [H00001729-M01] - Detection limit for recombinant GST tagged DIAPH1 is approximately 0.1ng/ml as a capture antibody.")
![ELISA: Human DIAPH1 ELISA Kit (Colorimetric) [NBP3-31406] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31406_human-diaph1-elisa-kit-colorimetric-206202415252437.png "ELISA: Human DIAPH1 ELISA Kit (Colorimetric) [NBP3-31406] - Standard Curve Reference")
![ELISA: Human DIAPH1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31407] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31407_human-diaph1-ready-to-use-elisa-kit-colorimetric-206202415351055.png "ELISA: Human DIAPH1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31407] - Standard Curve Reference")