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![Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NBP1-31386-img0017.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Dopamine beta hydroxylase antibody and a competitor's antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - RT2 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0013.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - U87 xenograft. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0011.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta hydroxylase antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP1-31386-img0012.jpg)
![Dopamine beta-Hydroxylase Antibody [NBP1-31386] - Mouse brain. Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-NBP1-31386-img0018.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human adrenal gland, kidney, lower gastrointestinal and testis using Anti-Dopamine beta-Hydroxylase antibody NBP2-57836 (A) shows similar protein distribution across tissues to independent antibody NBP2-55677 (B).](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57836-img0007.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0018.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0013.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human colon shows moderate cytoplasmic positivity in peripheral ganglion.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0015.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Staining of human kidney shows very weak positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-57836-img0017.jpg)
![Western Blot: Dopamine beta-Hydroxylase Antibody [NBP2-57836] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/antibody/nbp2-57836_rabbit-polyclonal-dopamine-beta-hydroxylase-antibody-western-blot-2120249955..jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-55677-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Analysis in human adrenal gland and testis tissues. Corresponding Dopamine beta-Hydroxylase RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Staining of human adrenal gland, kidney, lower gastrointestinal and testis using Anti-Dopamine beta-Hydroxylase antibody NBP2-55677 (A) shows similar protein distribution across tissues to independent antibody NBP2-57836 (B).](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-55677-img0015.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-55677-img0017.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-55677-img0012.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Staining of human colon shows strong cytoplasmic positivity in peripheral ganglion.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-55677-img0014.jpg)
![Immunohistochemistry-Paraffin: Dopamine beta-Hydroxylase Antibody [NBP2-55677] - Staining of human kidney shows no weak positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Immunohistochemistry-Paraffin-NBP2-55677-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25442_rabbit-dopamine-beta-hydroxylase-mab-hl1163-azide-bsa-free-2120249425432.jpg "Immunocytochemistry/Immunofluorescence: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Dopamine beta Hydroxylase antibody [HL1163] detects Dopamine beta Hydroxylase protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine beta Hydroxylase stained by Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25442_rabbit-dopamine-beta-hydroxylase-mab-hl1163-azide-bsa-free-2120249393558.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Dopamine beta-Hydroxylase Antibody (HL1163) - Azide and BSA Free [NBP3-25442] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with Dopamine beta Hydroxylase antibody [HL1163] (NBP3-25442) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25442_rabbit-dopamine-beta-hydroxylase-mab-hl1163-azide-bsa-free-2120249371522.jpg)
![Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Western blot of human adrenal medulla. The antibody is specific for the ~75 kDa DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-112-img0002.jpg "Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Western blot of human adrenal medulla. The antibody is specific for the ~75 kDa DBH protein.")
![Western Blot: Dopamine beta Hydroxylase Antibody [NB300-112] - Human adrenal medulla lysate showing specific immunolabeling of the ~75k DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-112-img0001.jpg)
![Western Blot: Dopamine beta-Hydroxylase Antibody [NB300-111] - Western blot of human adrenal medulla. The antibody is specific for the ~75kDa DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-111-img0006.jpg "Western Blot: Dopamine beta-Hydroxylase Antibody [NB300-111] - Western blot of human adrenal medulla. The antibody is specific for the ~75kDa DBH protein.")
![Western Blot: Dopamine beta-Hydroxylase Antibody [NB300-111] - Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75k DBH protein.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-NB300-111-img0005.jpg)
![ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Chemiluminescence) [NBP2-67944] - Samples were spiked with high concentrations of Human Dopamine beta-Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67944_human-dopamine-beta-hydroxylase-elisa-kit-chem-132202416201917.jpg "ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Chemiluminescence) [NBP2-67944] - Samples were spiked with high concentrations of Human Dopamine beta-Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Chemiluminescence) [NBP2-67944] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67944_human-dopamine-beta-hydroxylase-elisa-kit-chem-4320249151610.jpg)
![ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) [NBP3-18755] - Samples were spiked with high concentrations of Human Dopamine beta-Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18755_human-dopamine-beta-hydroxylase-elisa-kit-colorimetric-132202416133619.jpg "ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) [NBP3-18755] - Samples were spiked with high concentrations of Human Dopamine beta-Hydroxylase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Dopamine beta-Hydroxylase ELISA Kit (Colorimetric) [NBP3-18755] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18755_human-dopamine-beta-hydroxylase-elisa-kit-colorimetric-432024983584.jpg)
![Western Blot: Dopamine beta Hydroxylase Overexpression Lysate (Adult Normal) [NBL1-09727] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dopamine beta Hydroxylase.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09727-img0002.jpg "Western Blot: Dopamine beta Hydroxylase Overexpression Lysate (Adult Normal) [NBL1-09727] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Dopamine beta Hydroxylase.")
![Western Blot: Dopamine beta-Hydroxylase Antibody [H00001621-B01P] - Analysis of DBH expression in transfected 293T cell line by DBH polyclonal antibody. Lane 1: DBH transfected lysate(66.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Dopamine-beta-Hydroxylase-Antibody-Western-Blot-H00001621-B01P-img0003.jpg "Western Blot: Dopamine beta-Hydroxylase Antibody [H00001621-B01P] - Analysis of DBH expression in transfected 293T cell line by DBH polyclonal antibody. Lane 1: DBH transfected lysate(66.33 KDa). Lane 2: Non-transfected lysate.")