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![Knockdown Validated: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-SS-53-Western-Blot-NB500-212-img0002.jpg "<b>Genetic Strategies Validation. </b>Knockdown Validated: CHAF1B Antibody (SS 53) [NB500-212] - A. RKO cells with CAF-1 p150, CAF-1 p60 and PCNA antibodies. B. p150 RNAi knockdown. Reference Pubmed 14519857.")
![Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using NB500-211.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-SS-24-1-68-Western-Blot-NB500-211-img0001.jpg "Western Blot: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Analysis of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates using NB500-211.")
![Immunocytochemistry/Immunofluorescence: CHAF1B Antibody (SS 24 1-68) [NB500-211] - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).](http://images.novusbio.com/fullsize/CHAF1B-Antibody-SS-24-1-68-Immunocytochemistry-Immunofluorescence-NB500-211-img0004.jpg)
![Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Western-Blot-NBP2-46274-img0005.jpg "Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B.")
![Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunohistochemistry-NBP2-46274-img0004.jpg)
![Western Blot: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Western-Blot-NBP2-46274-img0006.jpg)
![Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunohistochemistry-NBP2-46274-img0002.jpg)
![Immunohistochemistry: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunohistochemistry-NBP2-46274-img0003.jpg)
![Immunofluorescence: CHAF1B Antibody (OTI4F7) [NBP2-46274] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-OTI4F7-Immunofluorescence-NBP2-46274-img0001.jpg)
![Western Blot: CHAF1B Antibody [NBP1-88235] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Western-Blot-NBP1-88235-img0006.jpg "Western Blot: CHAF1B Antibody [NBP1-88235] - Analysis in human cell line MOLT-4.")
![Immunocytochemistry/Immunofluorescence: CHAF1B Antibody [NBP1-88235] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88235-img0007.jpg)
![Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-Paraffin-NBP1-88235-img0010.jpg)
![Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-Paraffin-NBP1-88235-img0008.jpg)
![Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235] - Staining of human lymph nodes shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-Paraffin-NBP1-88235-img0009.jpg)
![Immunohistochemistry-Paraffin: CHAF1B Antibody [NBP1-88235] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-Paraffin-NBP1-88235-img0011.jpg)
![Western Blot: CHAF1B Antibody [NB100-57523] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-57523 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CAF-1 p60 was also immunoprecipitated by rabbit anti-CAF-1 p60 antibody NB100-57522, which recognizes an upstream epitope. Test](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Western-Blot-NB100-57523-img0002.jpg "<b>Independent Antibodies Validation, Biological Strategies Validation, Orthogonal Strategies Validation and Expression of Tagged Proteins Validation.</b>Western Blot: CHAF1B Antibody [NB100-57523] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. NB100-57523 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. CAF-1 p60 was also immunoprecipitated by rabbit anti-CAF-1 p60 antibody NB100-57522, which recognizes an upstream epitope. Test")
![<b>Independent Antibodies Validation, Biological Strategies Validation, Orthogonal Strategies Validation and Expression of Tagged Proteins Validation.</b>Immunohistochemistry: CHAF1B Antibody [NB100-57523] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-NB100-57523-img0004.jpg)
![Immunohistochemistry-Paraffin: CHAF1B Antibody [NB100-57523] - FFPE section of human seminoma. Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-Immunohistochemistry-Paraffin-NB100-57523-img0001.jpg)
![Western Blot: CHAF1B Antibody (7N0I2) [NBP3-16545] - Western blot analysis of extracts of various cell lines, using CHAF1B Rabbit mAb (NBP3-16545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-7N0I2-Western-Blot-NBP3-16545-img0002.jpg "Western Blot: CHAF1B Antibody (7N0I2) [NBP3-16545] - Western blot analysis of extracts of various cell lines, using CHAF1B Rabbit mAb (NBP3-16545) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: CHAF1B Antibody (7N0I2) [NBP3-16545] - Immunohistochemistry of paraffin-embedded human colon carcinoma using CHAF1B Rabbit mAb (NBP3-16545) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CHAF1B-Antibody-7N0I2-Immunohistochemistry-Paraffin-NBP3-16545-img0001.jpg)
![SDS-Page: Recombinant Human CHAF1B Protein [H00008208-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00008208-P01-1.jpg "SDS-Page: Recombinant Human CHAF1B Protein [H00008208-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: CHAF1B Overexpression Lysate (Adult Normal) [NBL1-09140] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHAF1B.](http://images.novusbio.com/fullsize/CHAF1B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09140-img0002.jpg "Western Blot: CHAF1B Overexpression Lysate (Adult Normal) [NBL1-09140] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHAF1B.")