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![Western Blot: tropomyosin-2 Antibody [NBP2-95197] - Analysis of extracts of various cell lines, using tropomyosin-2 at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Western-Blot-NBP2-95197-img0005.jpg "Western Blot: tropomyosin-2 Antibody [NBP2-95197] - Analysis of extracts of various cell lines, using tropomyosin-2 at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-95197] - Rat heart using TPM2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-95197-img0004.jpg)
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-95197] - Human stomach using TPM2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-95197-img0002.jpg)
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-95197] - Mouse heart using TPM2 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-95197-img0003.jpg)
![Immunoprecipitation: tropomyosin-2 Antibody [NBP2-95197] - Analysis of 300ug extracts of Mouse skeletal muscle cells using 3ug TPM2 antibody. Western blot was performed from the immunoprecipitate using TPM2 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunoprecipitation-NBP2-95197-img0006.jpg)
![Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunofluorescence analysis of paraffin-embedded human striated muscle tissue labeling tropomyosin-2 with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33059, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33059_rabbit-tropomyosin-2-mab-psh02-63-298202414262682.jpg "Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunofluorescence analysis of paraffin-embedded human striated muscle tissue labeling tropomyosin-2 with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33059, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33059) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33059_rabbit-tropomyosin-2-mab-psh02-63-29820241435247.jpg)
![Western Blot: tropomyosin-2 Antibody (PSH02-63) [NBP3-33059] - Western blot analysis of tropomyosin-2 on different lysates with Rabbit anti-tropomyosin-2 antibody (NBP3-33059) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate Lane 2: Saos-2 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: C2C12 cell lysate Lane 5: Mouse skeletal muscle tissue lysate Lane 6: Rat skeletal muscle tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 37 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33059_rabbit-tropomyosin-2-mab-psh02-63-392024848500.jpg)
![Western Blot: tropomyosin-2 Antibody [NBP1-32770] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Western-Blot-NBP1-32770-img0004.jpg "Western Blot: tropomyosin-2 Antibody [NBP1-32770] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP1-32770] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-32770-img0003.jpg)
![Western Blot: tropomyosin-2 Antibody [NBP1-57612] - HepG2 cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Western-Blot-NBP1-57612-img0004.jpg "Western Blot: tropomyosin-2 Antibody [NBP1-57612] - HepG2 cell lysate, concentration 2.5 ug/ml.")
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP1-57612] - Human Muscle Tissue, Skeletal muscle cells (Indicated with Arrows) 4-8ug/ml.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP1-57612-img0003.jpg)
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-99519] - Immunochemical staining tropomyosin-2 in rat skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99519-img0003.jpg "Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-99519] - Immunochemical staining tropomyosin-2 in rat skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-99519] - Immunochemical staining tropomyosin-2 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99519-img0001.jpg)
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody [NBP2-99519] - Immunochemical staining tropomyosin-2 in mouse skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-99519-img0002.jpg)
![Immunohistochemistry-Paraffin: tropomyosin-2 Antibody (3C8) [H00007169-M07] - Analysis of monoclonal antibody to TPM2 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-3C8-Immunohistochemistry-Paraffin-H00007169-M07-img0002.jpg "Immunohistochemistry-Paraffin: tropomyosin-2 Antibody (3C8) [H00007169-M07] - Analysis of monoclonal antibody to TPM2 on formalin-fixed paraffin-embedded human smooth muscle. Antibody concentration 3 ug/ml")
![ELISA: tropomyosin-2 Antibody (3C8) [H00007169-M07] - Detection limit for recombinant GST tagged TPM2 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/tropomyosin-2-Antibody-3C8-ELISA-H00007169-M07-img0001.jpg)
![Western Blot: tropomyosin-2 Overexpression Lysate (Adult Normal) [NBP2-10870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for tropomyosin-2.](http://images.novusbio.com/fullsize/tropomyosin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10870-img0001.jpg "Western Blot: tropomyosin-2 Overexpression Lysate (Adult Normal) [NBP2-10870] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for tropomyosin-2.")
![Western Blot: tropomyosin-2 Overexpression Lysate (Adult Normal) [NBP2-04458] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for tropomyosin-2.](http://images.novusbio.com/fullsize/tropomyosin-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04458-img0001.jpg "Western Blot: tropomyosin-2 Overexpression Lysate (Adult Normal) [NBP2-04458] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for tropomyosin-2.")
![SDS-Page: tropomyosin-2 Protein [NBP1-50957]](http://images.novusbio.com/fullsize/tropomyosin-2-Protein-SDS-Page-NBP1-50957-img0001.jpg "SDS-Page: tropomyosin-2 Protein [NBP1-50957]")